##gff-version 3
##sequence-region BBa_K182101 1 960
BBa_K182101	.	terminator	920	960	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K182101	.	stem_loop	927	946	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K182101	.	stop_codon	953	953	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K182101	.	polyA_site	947	960	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K182101	.	engineered_region	920	960	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K182101	.	CDS	104	823	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K182101	.	CDS	104	823	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K182101	.	ribosome_entry_site	83	97	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K182101	.	ribosome_entry_site	90	94	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K182101	.	engineered_region	83	97	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K182101	.	ribosome_entry_site	83	97	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K182101	.	promoter	1	74	.	+	0	ID=BBa_I751502;Name=BBa_I751502
BBa_K182101	.	sequence_feature	42	48	.	+	0	ID=annotation1955561;Name=-10;Parent=BBa_I751502
BBa_K182101	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1955564;Name=luxR binding site;Parent=BBa_I751502
BBa_K182101	.	sequence_feature	20	26	.	+	0	ID=annotation1955560;Name=-35;Parent=BBa_I751502
BBa_K182101	.	promoter	20	74	.	+	0	ID=annotation1955559;Name=plac;Parent=BBa_I751502
BBa_K182101	.	non_covalent_binding_site	49	74	.	+	0	ID=annotation1955563;Name=O1;Parent=BBa_I751502
BBa_K182101	.	non_covalent_binding_site	27	41	.	+	0	ID=annotation1955562;Name=O1;Parent=BBa_I751502
BBa_K182101	.	terminator	832	911	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K182101	.	stem_loop	843	886	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K182101	.	engineered_region	832	911	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K182101
acctgtaggatcgtacaggtttacttgtgagcggataacaatatagtgtgtggaattgtgagcggataacaatttacta
gagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattag
atggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaa
atttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgcttt
gcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaacta
tatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgta
tacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttc
aactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccac
acaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggatt
acacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttata