BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_I751502
1
BBa_I751502
plux-lac hybrid promoter
2007-10-24T11:00:00Z
2015-08-31T04:08:06Z
sense TWO INPUTS, activation by AHL and repression by LacI.
false
false
_151_
0
2188
9
Not in stock
false
Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI.
Also, the output is clearly detected as shown in Fig. 3.
false
Kenichiro Iwasaki
annotation1955563
1
O1
range1955563
1
49
74
annotation1955559
1
plac
range1955559
1
20
74
annotation1955564
1
luxR binding site
range1955564
1
1
19
annotation1955561
1
-10
range1955561
1
42
48
annotation1955562
1
O1
range1955562
1
27
41
annotation1955560
1
-35
range1955560
1
20
26
BBa_K182102
1
BBa_K182102
pLux-Lac with RBS attached
2009-07-06T11:00:00Z
2015-05-08T01:11:06Z
BBa_I751502 and BBa_B0034)
pLux-Lac hybrid promoter (BBa_I751502) upstream with RBS (BBa_B0034) attached downstream.
false
false
_279_
0
4115
9
It's complicated
true
N/A atm
false
Calum de Burgh and Zuzana Hruskova
component2010165
1
BBa_B0034
component2010163
1
BBa_I751502
annotation2010165
1
BBa_B0034
range2010165
1
83
94
annotation2010163
1
BBa_I751502
range2010163
1
1
74
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K182102_sequence
1
acctgtaggatcgtacaggtttacttgtgagcggataacaatatagtgtgtggaattgtgagcggataacaatttactagagaaagaggagaaa
BBa_I751502_sequence
1
acctgtaggatcgtacaggtttacttgtgagcggataacaatatagtgtgtggaattgtgagcggataacaatt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z