LOCUS       BBa_K182103             1761 bp    DNA     linear       17-May-2024
DEFINITION  Composite part of K182100 and K182102
ACCESSION   BBa_K182103
VERSION     BBa_K182103.1  
FEATURES             Location/Qualifiers
     promoter        20..74
                     /label=plac
     misc_feature    20..26
                     /label=-35
     misc_feature    42..48
                     /label=-10
     misc_feature    27..41
                     /label=O1
     misc_feature    49..74
                     /label=O1
     misc_feature    1..19
                     /label=luxR binding site
     promoter        1..74
                     /label=BBa_I751502
     misc_feature    87..90
                     /label=conserved
     RBS             83..94
                     /label=BBa_B0034
     promoter        1..94
                     /label=BBa_K182102
     CDS             104..811
                     /label=cI lambda
     misc_feature    812..844
                     /label=LVA
     misc_feature    851..875
                     /label=Help:Barcodes
     CDS             101..875
                     /label=BBa_C0051
     RBS             884..898
                     /label=RBS-1\Strong
     RBS             891..895
                     /label=RBS
     misc_feature    884..898
                     /label=BBa_B0030
     RBS             884..898
                     /label=BBa_B0030
     CDS             905..1624
                     /label=GFP protein
     CDS             905..1624
                     /label=BBa_E0040
     stem_loop       1644..1687
                     /label=stem_loop
     misc_feature    1633..1712
                     /label=BBa_B0010
     terminator      1633..1712
                     /label=BBa_B0010
     stem_loop       1728..1747
                     /label=T7 TE
     misc_feature    1754..1754
                     /label=stop
     polyA_site      1748..1761
                     /label=polya
     misc_feature    1721..1761
                     /label=BBa_B0012
     terminator      1721..1761
                     /label=BBa_B0012
     misc_feature    884..1761
                     /label=BBa_E0840
     misc_feature    101..1761
                     /label=BBa_K182100
ORIGIN
        1 acctgtagga tcgtacaggt ttacttgtga gcggataaca atatagtgtg tggaattgtg
       61 agcggataac aatttactag agaaagagga gaaatactag atgagcacaa aaaagaaacc
      121 attaacacaa gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa
      181 aaatgaactt ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg
      241 cgttggtgct ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc
      301 aaaaattctc aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga
      361 gatgtatgaa gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt
      421 ttctcatgtt caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc
      481 ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga
      541 aggtaattcc atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat
      601 tctcgttgac cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg
      661 tgatgagttt accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact
      721 aaacccacag tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat
      781 cgctagtcag tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt
      841 agcttaataa cgctgatagt gctagtgtag atcgctacta gagattaaag aggagaaata
      901 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      961 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
     1021 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
     1081 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
     1141 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
     1201 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
     1261 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
     1321 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
     1381 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     1441 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     1501 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     1561 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
     1621 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1681 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     1741 gtgggccttt ctgcgtttat a
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