##gff-version 3
##sequence-region BBa_K182103 1 1761
BBa_K182103	.	promoter	1	94	.	+	0	ID=BBa_K182102;Name=BBa_K182102
BBa_K182103	.	ribosome_entry_site	83	94	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K182102
BBa_K182103	.	conserved_region	87	90	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K182103	.	promoter	1	74	.	+	0	ID=BBa_I751502;Name=BBa_I751502;Parent=BBa_K182102
BBa_K182103	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1955564;Name=luxR binding site;Parent=BBa_I751502
BBa_K182103	.	non_covalent_binding_site	27	41	.	+	0	ID=annotation1955562;Name=O1;Parent=BBa_I751502
BBa_K182103	.	sequence_feature	42	48	.	+	0	ID=annotation1955561;Name=-10;Parent=BBa_I751502
BBa_K182103	.	sequence_feature	20	26	.	+	0	ID=annotation1955560;Name=-35;Parent=BBa_I751502
BBa_K182103	.	promoter	20	74	.	+	0	ID=annotation1955559;Name=plac;Parent=BBa_I751502
BBa_K182103	.	non_covalent_binding_site	49	74	.	+	0	ID=annotation1955563;Name=O1;Parent=BBa_I751502
BBa_K182103	.	engineered_region	101	1761	.	+	0	ID=BBa_K182100;Name=BBa_K182100
BBa_K182103	.	CDS	101	875	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K182100
BBa_K182103	.	tag	812	844	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K182103	.	engineered_tag	851	875	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K182103	.	CDS	104	811	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K182103	.	engineered_region	884	1761	.	+	0	ID=BBa_E0840;Name=BBa_E0840;Parent=BBa_K182100
BBa_K182103	.	ribosome_entry_site	884	898	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K182103	.	engineered_region	884	898	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K182103	.	ribosome_entry_site	884	898	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K182103	.	ribosome_entry_site	891	895	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K182103	.	CDS	905	1624	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K182103	.	CDS	905	1624	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K182103	.	terminator	1633	1712	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K182103	.	stem_loop	1644	1687	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K182103	.	engineered_region	1633	1712	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K182103	.	terminator	1721	1761	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K182103	.	engineered_region	1721	1761	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K182103	.	stop_codon	1754	1754	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K182103	.	polyA_site	1748	1761	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K182103	.	stem_loop	1728	1747	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_K182103
acctgtaggatcgtacaggtttacttgtgagcggataacaatatagtgtgtggaattgtgagcggataacaatttacta
gagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgcctta
aagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtc
aggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagtt
agcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcactta
gaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaagg
tgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatg
accgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccag
gtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgttttt
acaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcag
tggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgta
gatcgctactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattct
tgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaa
cttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtg
ttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtaca
ggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgtt
aatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataact
cacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaaga
tggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccat
tacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacag
ctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata