##gff-version 3
##sequence-region BBa_K182200 1 1651
BBa_K182200	.	engineered_region	44	707	.	+	0	ID=BBa_K081008;Name=BBa_K081008
BBa_K182200	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081008
BBa_K182200	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K182200	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K182200	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K182200	.	CDS	65	682	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K081008
BBa_K182200	.	tag	644	675	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K182200	.	engineered_tag	683	707	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K182200	.	engineered_region	65	682	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K182200	.	CDS	65	643	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K182200	.	engineered_region	716	1651	.	+	0	ID=BBa_I0462;Name=BBa_I0462
BBa_K182200	.	CDS	734	1489	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K182200	.	engineered_tag	1490	1514	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K182200	.	engineered_region	734	735	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K182200	.	CDS	734	1483	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K182200	.	point_mutation	907	907	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K182200	.	engineered_region	734	1489	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K182200	.	point_mutation	1225	1225	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K182200	.	ribosome_entry_site	716	727	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K182200	.	conserved_region	720	723	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K182200	.	terminator	1611	1651	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K182200	.	stem_loop	1618	1637	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K182200	.	engineered_region	1611	1651	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K182200	.	stop_codon	1644	1644	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K182200	.	polyA_site	1638	1651	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K182200	.	terminator	1523	1602	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K182200	.	engineered_region	1523	1602	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K182200	.	stem_loop	1534	1577	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K182200	.	promoter	1	35	.	+	0	ID=BBa_J23107;Name=BBa_J23107
>BBa_K182200
tttacggctagctcagccctaggtattatgctagctactagagattaaagaggagaaatactagatgactataatgata
aaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaa
gacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgc
ttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgttttt
cctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaata
gctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaagg
tattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtatt
ggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaag
cagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctact
agagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgt
agaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatca
tttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatga
cgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaa
aacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcc
ctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatt
tttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataat
aaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatattt
caaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccg
ctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgc
tagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata