BBa_K1823001 1 BBa_K1823001 EDE1C8 TEV scFv 2015-09-17T11:00:00Z 2015-09-18T01:11:54Z gene fragment synthesis EDE1C8 is one of broadly neutralize antibody for envelope glycoprotein of Dengue virus. This antibody this came from patients who infected by unknown serotype of Dengue virus,in silico experiment prove that the antibody can bind to all dengue virus envelope glycoprotein. To express this antibody in your choice host cell, such as E. coli, add the promoter and RBS on the upstream site. This part also has OsmY as a signal peptide that will bring the antibody to periplasm, so, it can be isolate by osmotic shock. You can collect the antibody by purification using Nickel column, it has Histidine (6X), and cut the target protein from another peptide using TEV protease. This scFv antibody has Myc as the linker between the light chain and heavy chain. false false _2249_ 25928 25928 9 false OsmY, GSGG, Histidine (6X), TEV, vL, Myc, vH, terminator false Isyatul Azizah annotation2469817 1 Myc range2469817 1 707 737 annotation2469657 1 osmY range2469657 1 82 283 annotation2469656 1 TEV range2469656 1 316 334 annotation2469662 1 vH range2469662 1 335 706 annotation2469659 1 Histidine range2469659 1 297 315 annotation2469658 1 GSGG range2469658 1 284 296 annotation2469848 1 vL range2469848 1 738 1444 BBa_K1823001_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacaattaaagaggagaaaatgacgatgacccgcctgaaaatctctaagacccttttagcagtcatgttgaccagcgctgtagctacgggcagcgcctatgccgaaaacaatgcgcagaccaccaatgaaagcgcgggtcagaaagtcgactcctccatgaataaggtgggtaattttatggacgacagtgccatcaccgcgaaagtgaaggcagccctggtggatcacgataacattaaatccactgatattagcgtgaaaacagatcaaaaagtagttacgctgagcggctttgtggaatcacaagcacaggcagaggaggctgtaaaggtagcaaaaggggtagaaggcgtgacgtcagtctcggataaattgcatgttcgggacgccaaagaaggatcagtcaagggttatgcgggcgatacagcaacaacttctgaaattaaagctaaactgttagccgatgacatcgtgccgagccggcacgtgaaagtggaaacgaccgatggtgtagtacagctgagcgggacagtggactcccaagctcagtctgatcgcgccgagtctattgcgaaagctgtggatggggtaaaaagcgttaaaaatgatctgaaaaccaaaggcagcggtggacaccatcaccaccatcatgaaaacctgtattttcagtcggaagtgcagcttgtcgagtctggcggggggttagtgcaaccgggtggtagcctgcgtttatcgtgttccgcctcagggttcacgttctcgacctacagtatgcactgggtgcggcaggcaccgggtaagggtttggaatatgtctcagcgattacaggtgaaggtgacagcgccttttacgcagactcggtcaaaggacgcttcaccatttctcgtgacaattctaagaacaccctgtactttgaaatgaactctctgcgcccggaggatacggcggtgtattattgtgtcggcggctacagcaacttttactactactatacaatggatgtttggggtcaaggtactactgtaaccgtcgaacagaaacttattagcgaagaggacctggagatcgtactgacccagagtccggcgacgttaagtctcagccccggcgagcgtgccactctgagctgtcgcgcgagccaaagcattagcacgtttttggcgtggtaccagcataaaccgggacaagcgccgcgcctgcttatctacgatgcctcgacccgtgccactggagtaccggcgcgcttttctggctcccgcagcggtaccgattttactttaaccatctcgaccctcgagcccgaagatttcgctgtttactattgtcaacagcgttataattggcctccgtataccttcggtcaaggcaccaaagttgaaatcaagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z