BBa_K1823001
1
BBa_K1823001
EDE1C8 TEV scFv
2015-09-17T11:00:00Z
2015-09-18T01:11:54Z
gene fragment synthesis
EDE1C8 is one of broadly neutralize antibody for envelope glycoprotein of Dengue virus. This antibody this came from patients who infected by unknown serotype of Dengue virus,in silico experiment prove that the antibody can bind to all dengue virus envelope glycoprotein.
To express this antibody in your choice host cell, such as E. coli, add the promoter and RBS on the upstream site. This part also has OsmY as a signal peptide that will bring the antibody to periplasm, so, it can be isolate by osmotic shock.
You can collect the antibody by purification using Nickel column, it has Histidine (6X), and cut the target protein from another peptide using TEV protease.
This scFv antibody has Myc as the linker between the light chain and heavy chain.
false
false
_2249_
25928
25928
9
false
OsmY, GSGG, Histidine (6X), TEV, vL, Myc, vH, terminator
false
Isyatul Azizah
annotation2469659
1
Histidine
range2469659
1
297
315
annotation2469658
1
GSGG
range2469658
1
284
296
annotation2469817
1
Myc
range2469817
1
707
737
annotation2469662
1
vH
range2469662
1
335
706
annotation2469657
1
osmY
range2469657
1
82
283
annotation2469656
1
TEV
range2469656
1
316
334
annotation2469848
1
vL
range2469848
1
738
1444
BBa_K1823001_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacaattaaagaggagaaaatgacgatgacccgcctgaaaatctctaagacccttttagcagtcatgttgaccagcgctgtagctacgggcagcgcctatgccgaaaacaatgcgcagaccaccaatgaaagcgcgggtcagaaagtcgactcctccatgaataaggtgggtaattttatggacgacagtgccatcaccgcgaaagtgaaggcagccctggtggatcacgataacattaaatccactgatattagcgtgaaaacagatcaaaaagtagttacgctgagcggctttgtggaatcacaagcacaggcagaggaggctgtaaaggtagcaaaaggggtagaaggcgtgacgtcagtctcggataaattgcatgttcgggacgccaaagaaggatcagtcaagggttatgcgggcgatacagcaacaacttctgaaattaaagctaaactgttagccgatgacatcgtgccgagccggcacgtgaaagtggaaacgaccgatggtgtagtacagctgagcgggacagtggactcccaagctcagtctgatcgcgccgagtctattgcgaaagctgtggatggggtaaaaagcgttaaaaatgatctgaaaaccaaaggcagcggtggacaccatcaccaccatcatgaaaacctgtattttcagtcggaagtgcagcttgtcgagtctggcggggggttagtgcaaccgggtggtagcctgcgtttatcgtgttccgcctcagggttcacgttctcgacctacagtatgcactgggtgcggcaggcaccgggtaagggtttggaatatgtctcagcgattacaggtgaaggtgacagcgccttttacgcagactcggtcaaaggacgcttcaccatttctcgtgacaattctaagaacaccctgtactttgaaatgaactctctgcgcccggaggatacggcggtgtattattgtgtcggcggctacagcaacttttactactactatacaatggatgtttggggtcaaggtactactgtaaccgtcgaacagaaacttattagcgaagaggacctggagatcgtactgacccagagtccggcgacgttaagtctcagccccggcgagcgtgccactctgagctgtcgcgcgagccaaagcattagcacgtttttggcgtggtaccagcataaaccgggacaagcgccgcgcctgcttatctacgatgcctcgacccgtgccactggagtaccggcgcgcttttctggctcccgcagcggtaccgattttactttaaccatctcgaccctcgagcccgaagatttcgctgtttactattgtcaacagcgttataattggcctccgtataccttcggtcaaggcaccaaagttgaaatcaagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z