BBa_K1823002
1
BBa_K1823002
EDE1C8 scFv
2015-09-17T11:00:00Z
2015-09-18T01:56:04Z
gene fragment synthesis
EDE1C8 is one of broadly neutralize antibody for envelope glycoprotein of Dengue virus. This antibody this came from patients who infected by unknown serotype of Dengue virus,in silico experiment prove that the antibody can bind to all dengue virus envelope glycoprotein.
To express this antibody in your choice host cell, such as E. coli, add the promoter and RBS on the upstream site. This part also has OsmY as a signal peptide that will bring the antibody to periplasm, so, it can be isolate by osmotic shock.
This scFv antibody has Myc as the linker between the light chain and heavy chain.
false
false
_2249_
25928
25928
9
false
osmY, GSGG, Histidine (6X), vH, Myc,vL, terminator
false
Isyatul Azizah
annotation2469888
1
Myc
range2469888
1
1006
1036
annotation2469885
1
Histidine
range2469885
1
624
642
annotation2469882
1
osmY
range2469882
1
7
610
annotation2469883
1
GSGG
range2469883
1
611
623
annotation2469887
1
vH
range2469887
1
642
1005
annotation2469889
1
vL
range2469889
1
1037
1361
BBa_K1823002_sequence
1
aggagaaaatgacgatgacgcggctcaagatctcgaaaaccttacttgcagtaatgctgacaagcgcggttgcgacgggttcggcctatgcagaaaataatgcccaaacaaccaatgaatccgctggtcagaaagtagacagttcgatgaacaaagtcgggaactttatggacgattcggctattaccgcgaaggtgaaggcagcgcttgtggaccatgataatatcaaatcgacggacattagtgtcaagaccgatcaaaaagttgtcacgttatccggtttcgttgaatcgcaggcacaggcggaggaagcagttaaagttgccaaaggcgtcgaaggcgttaccagcgtgtctgataaactgcacgtgcgtgatgccaaagagggcagcgtaaaagggtatgccggtgacacggctactacctcagaaatcaaggctaagctgctggcggatgatattgttccttctcgccatgttaaggttgagacaaccgatggcgttgtacagctgtctggcaccgtcgactcccaagcgcagtcagatcgcgctgaatctatcgcgaaagcagttgatggcgttaaaagcgtgaaaaacgatttgaaaacaaaaggtagcgggggccatcaccatcaccaccatgaggtgcaactggttgaatcgggcggcgggctggtacagccgggtggtagtttgcgtctgagttgtagcgcgtctggtttcacctttagcacatactctatgcactgggtccgccaagcgcccggcaaaggtttggagtacgtgtcagctattacgggggagggtgactcggccttttacgcggatagcgttaagggccgcttcaccatttctcgcgataatagtaagaacacgctttatttcgagatgaactcactccggcccgaagataccgctgtgtattactgcgtaggcggatacagcaacttttactactattacacgatggatgtgtggggccaggggaccaccgtgactgtggagcagaaactgatttcggaagaggatttggaaatcgttctgacccaatcgcccgctactttgtcgctgagccctggcgagcgggcgacgctgagctgccgtgctagccagagcatctcgaccttcctggcatggtaccaacacaaaccaggccaggctccacgccttctcatctacgatgccagtacccgtgccacaggggtgccggcacgtttttctggtagtcgcagtggcacagatttcacgcttaccatcagtactctggagcctgaagatttcgcagtatattactgtcaacaacgctacaactggcccccttataccttcgggcaaggcactaaagtagaaattaaataatcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z