BBa_K1823003 1 BBa_K1823003 EDE1C8 + promoter 2015-09-17T11:00:00Z 2015-09-18T02:25:37Z gene fragment synthesis EDE1C8 is one of broadly neutralize antibody for envelope glycoprotein of Dengue virus. This antibody this came from patients who infected by unknown serotype of Dengue virus,in silico experiment prove that the antibody can bind to all dengue virus envelope glycoprotein. It has promoter and RBS on the upstream site, also has OsmY as a signal peptide that will bring the antibody to periplasm, so, it can be isolate by osmotic shock. You can collect the antibody by purification using Nickel column, it has Histidine (6X). Note: This part does not have TEV sequence to cleave the targeted antibody and separate the antibody from another prtoein. This scFv antibody has Myc as the linker between the light chain and heavy chain. false false _2249_ 25928 25928 9 false osmY, GSGG, Histidine (6X), vH, Myc, vL false Isyatul Azizah annotation2469933 1 osmY range2469933 1 24 627 annotation2469956 1 vL range2469956 1 1045 1369 annotation2469953 1 Myc range2469953 1 1014 1044 annotation2469941 1 GSGG range2469941 1 628 630 annotation2469949 1 vH range2469949 1 650 1013 annotation2469943 1 Histidine range2469943 1 631 649 BBa_K1823003_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacaattaaagaggagaaaatgacgatgacgcggctcaagatctcgaaaaccttacttgcagtaatgctgacaagcgcggttgcgacgggttcggcctatgcagaaaataatgcccaaacaaccaatgaatccgctggtcagaaagtagacagttcgatgaacaaagtcgggaactttatggacgattcggctattaccgcgaaggtgaaggcagcgcttgtggaccatgataatatcaaatcgacggacattagtgtcaagaccgatcaaaaagttgtcacgttatccggtttcgttgaatcgcaggcacaggcggaggaagcagttaaagttgccaaaggcgtcgaaggcgttaccagcgtgtctgataaactgcacgtgcgtgatgccaaagagggcagcgtaaaagggtatgccggtgacacggctactacctcagaaatcaaggctaagctgctggcggatgatattgttccttctcgccatgttaaggttgagacaaccgatggcgttgtacagctgtctggcaccgtcgactcccaagcgcagtcagatcgcgctgaatctatcgcgaaagcagttgatggcgttaaaagcgtgaaaaacgatttgaaaacaaaaggtagcgggggccatcaccatcaccaccatgaggtgcaactggttgaatcgggcggcgggctggtacagccgggtggtagtttgcgtctgagttgtagcgcgtctggtttcacctttagcacatactctatgcactgggtccgccaagcgcccggcaaaggtttggagtacgtgtcagctattacgggggagggtgactcggccttttacgcggatagcgttaagggccgcttcaccatttctcgcgataatagtaagaacacgctttatttcgagatgaactcactccggcccgaagataccgctgtgtattactgcgtaggcggatacagcaacttttactactattacacgatggatgtgtggggccaggggaccaccgtgactgtggagcagaaactgatttcggaagaggatttggaaatcgttctgacccaatcgcccgctactttgtcgctgagccctggcgagcgggcgacgctgagctgccgtgctagccagagcatctcgaccttcctggcatggtaccaacacaaaccaggccaggctccacgccttctcatctacgatgccagtacccgtgccacaggggtgccggcacgtttttctggtagtcgcagtggcacagatttcacgcttaccatcagtactctggagcctgaagatttcgcagtatattactgtcaacaacgctacaactggcccccttataccttcgggcaaggcactaaagtagaaattaaataatcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z