BBa_K1824004 1 BBa_K1824004 pBAD + RNA Thermometer FourU 2015-09-04T11:00:00Z 2015-09-05T11:44:53Z FourU was previously submitted by TuDelft 2008. This is a composite regulatory part that consisting of pBAD BBa_I13453 promoter and RNA Thermometer FourU K1824560 for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted. false false _2250_ 25962 25962 9 false The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. It is important to avoid truncation or alteration of the RNA sequence false Wenbo Xu component2444203 1 BBa_I13453 component2444204 1 BBa_K1824560 annotation2444204 1 BBa_K1824560 range2444204 1 131 195 annotation2444203 1 BBa_I13453 range2444203 1 1 130 BBa_I13453 1 BBa_I13453 Pbad promoter 2005-05-24T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 PBad promoter from I0500 without AraC. false false _11_ 0 253 6 In stock false true jkm BBa_K1824560 1 BBa_K1824560 RNA Thermometer FourU (Specially designed for pBAD) 2015-09-04T11:00:00Z 2015-09-05T11:32:25Z FourU was previously submitted by TuDelft 2008. This is a specially designed A1 RNA thermometer that with a unique spacer at the front, which would make it compatible with pBAD BBa_I13453. A1 RNA thermometer have the hairpin structure that harbors the Shine-Dalgarno sequence (SD sequence) and, in this way, make it inaccessible to the 30S unit of the bacterial ribosome, resulting in translational inactivation (Figure 2). The melting temperature of this RNA thermometer is 37 Celsius degree. Once reaching the melting temperature, hairpin structure would vanish and as a result, exposing the SD sequence to trigger the translation process. Different promoters have their own transcription start sites and, in most cases, + 1 sites are embedded in promoter sequence. Hence, it is normal that transcribed RNA usually carry part of promoter sequence. However, for regulatory parts like RNA thermometer, truncation or alteration of the RNA sequence could be destructive. Hence, special designed RNA spacer between transcribed part of promoters and RNA thermometers are important for maintaining the secondary structure of RNA thermometer. For pBAD BBa_I13453, transcription starts at TCTCCATA (transcription start site indicated in bold). Based on this, BBa_K1824557 was specially designed with a spacer that had less probability to interact with the functional structure of RNA thermometer. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). For testing results of pBAD-A1, See BBa_K1824003. false false _2250_ 25962 25962 9 false To maintain the secondary structure of RNA thermometers, researchers need to be careful with of the use of any parts that couple with it. For instance, in some cases, part of operator region can be transcribed into RNAs and form tight secondary structure and thus interfere RNA thermometers. false Wenbo Xu BBa_I13453_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_K1824560_sequence 1 tactagagggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggtacta BBa_K1824004_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggtacta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z