BBa_K1824563 1 BBa_K1824563 RNA Thermometer U6 (Specially designed for pBAD) 2015-09-05T11:00:00Z 2015-09-06T05:08:17Z The DNA sequence of U6 was from registry. This is a specially designed U6 RNA thermometer that with a unique spacer at the front, which would make it compatible with promoter BBa_J23119. It is important to noted that this part is not compatible with RFC[10]. XJTLU-CHINA used Gibson method to assembly this part to others. U6 RNA thermometer have the hairpin structure that harbors the Shine-Dalgarno sequence (SD sequence) and, in this way, make it inaccessible to the 30S unit of the bacterial ribosome, resulting in translational inactivation (Figure 2). The melting temperature of this RNA thermometer is 37 Celsius degree. Once reaching the melting temperature, hairpin structure would vanish and as a result, exposing the SD sequence to trigger the translation process. Different promoters have their own transcription start sites and, in most cases, + 1 sites are embedded in promoter sequence. Hence, it is normal that transcribed RNA usually carry part of promoter sequence. However, for regulatory parts like RNA thermometer, truncation or alteration of the RNA sequence could be destructive. Hence, special designed RNA spacer between transcribed part of promoters and RNA thermometers are important for maintaining the secondary structure of RNA thermometer. For BBa_J23119, transcription starts at TAATGCTAGCA (transcription start site indicated in bold). Based on this, BBa_K1824562 was specially designed with a spacer that had less probability to interact with the functional structure of RNA thermometer. The possible secondary structure of U6 was simulated by RNAstructure (Fig.1). For testing results of J23119-U6, See BBa_K1824002. false false _2250_ 25962 25962 9 false To maintain the secondary structure of RNA thermometers, researchers need to be careful with of the use of any parts that couple with it. For instance, in some cases, part of operator region can be transcribed into RNAs and form tight secondary structure and thus interfere RNA thermometers. false Wenbo Xu BBa_I13453 1 BBa_I13453 Pbad promoter 2005-05-24T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 PBad promoter from I0500 without AraC. false false _11_ 0 253 6 In stock false true jkm BBa_K1824005 1 BBa_K1824005 pBAD + RNA Thermometer U6 2015-09-05T11:00:00Z 2015-09-06T05:22:36Z U6 was from registry. This is a composite regulatory part that consisting of pBAD BBa_I13453 promoter and RNA Thermometer U6 BBa_K1824563 for temperature induced gene expression at 37 degrees. The possible secondary structure of FourU was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted. false false _2250_ 25962 25962 9 false The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. It is important to avoid truncation or alteration of the RNA sequence false Wenbo Xu component2445044 1 BBa_I13453 component2445045 1 BBa_K1824563 annotation2445044 1 BBa_I13453 range2445044 1 1 130 annotation2445045 1 BBa_K1824563 range2445045 1 131 197 BBa_I13453_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_K1824005_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagggatcctctccttcactagtaaaaaaaaaaaaaaaaaaaaaaaaaaaggagatataccc BBa_K1824563_sequence 1 tactagagggatcctctccttcactagtaaaaaaaaaaaaaaaaaaaaaaaaaaaggagatataccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z