BBa_K1824006 1 BBa_K1824006 Consensus Promoter T7 + RNA Thermometer A1 2015-09-05T11:00:00Z 2015-09-17T07:15:49Z T7 promoter was from registry. This is a composite regulatory part that consisting of T7 promoter BBa_I13453 and RNA Thermometer A1 for temperature induced gene expression at 42 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted. false false _2250_ 25787 25962 9 false The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. It is important to avoid truncation or alteration of the RNA sequence false Wenbo Xu, Hao Wu component2445054 1 BBa_K1824558 component2445053 1 BBa_J64997 annotation2445053 1 BBa_J64997 range2445053 1 1 19 annotation2445054 1 BBa_K1824558 range2445054 1 20 71 BBa_K1824558 1 BBa_K1824558 RNA Thermometer A1 (Specially designed for T7) 2015-09-05T11:00:00Z 2015-09-06T06:08:32Z The DNA sequence of A1 was from BIT-CHINA. This is a specially designed A1 RNA thermometer that with a unique spacer at the front, which would make it compatible with T7. false false _2250_ 25962 25962 9 false To maintain the secondary structure of RNA thermometers, researchers need to be careful with of the use of any parts that couple with it. For instance, in some cases, part of operator region can be transcribed into RNAs and form tight secondary structure and thus interfere RNA thermometers. false Wenbo Xu BBa_J64997 1 BBa_J64997 T7 consensus -10 and rest 2007-03-25T11:00:00Z 2015-05-08T01:08:18Z false false _98_ 0 1428 98 Not in stock false false Sai Duriseti annotation1921746 1 T7-10 range1921746 1 1 19 BBa_J64997_sequence 1 taatacgactcactatagg BBa_K1824006_sequence 1 taatacgactcactataggacagagaacatactagagctctttaaaaaaaaaaagtactaaggagtactag BBa_K1824558_sequence 1 acagagaacatactagagctctttaaaaaaaaaaagtactaaggagtactag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z