BBa_J23105 1 BBa_J23105 constitutive promoter family member 2006-08-13T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1824009 1 BBa_K1824009 Constitutive promoter J23105 + Tet O + RBS 2015-09-05T11:00:00Z 2015-09-07T10:57:19Z J23105 and RBS B0034 were from registry. This part is a combination of Constitutive promoter J23105 and RBS B0034 false false _2250_ 25962 25962 9 false The TACTAG scar between promoter J23119 and RBS may influence protein production. false Zixu Wang component2445653 1 BBa_K1824890 component2445652 1 BBa_B0034 component2445650 1 BBa_K1824889 component2445649 1 BBa_J64991 component2445648 1 BBa_J23105 annotation2445650 1 BBa_K1824889 range2445650 1 55 62 annotation2445652 1 BBa_B0034 range2445652 1 63 74 annotation2445653 1 BBa_K1824890 range2445653 1 75 80 annotation2445648 1 BBa_J23105 range2445648 1 1 35 annotation2445649 1 BBa_J64991 range2445649 1 36 54 BBa_J64991 1 BBa_J64991 TetR 2007-03-25T11:00:00Z 2015-05-08T01:08:18Z false true _98_ 0 1428 98 Not in stock false false Sai Duriseti BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1824890 1 BBa_K1824890 The Sequence of the SpeI/XbaI scar in BioBricks standard assembly. 2015-09-06T11:00:00Z 2015-09-07T10:05:29Z It is the scar sequence of 3A assembly. In most cases, designers usually tend to embedded an appropriate spacer sequence between one individual part and the other. The 3A assembly, a commonly used manipulation method, creating scar between biobricks and, this unique scar usually served as default transcription spacer sequence. XJTLU-CHINA 2015 used Gibson assembly instead of conventional 3A assembly to create complex parts. If the reader have any interest to read the assembly diagram that the team promoted, they would find out that Gibson assembly was the ideal choices for the project. Though XJTLU-CHINA used Gibson assembly, which means parts could directly attach to one another without scars, the team still persevered the usually used scar sequence as the spacer. If readers looked through the assembly diagram that the team provided, they would find out that, for the constructs like a promoter part + a RBS part, the team not only embedded the spacer between the promoter and the RBS but also attach a spacer at the end of the RBS part. If the readers know the mechanism of Gibson assembly, they would know that it is for the one of the greatest feature of Gibson assembly, scarless manipulation. However, this design became problematical when the team registry the parts. Take the mentioned a promoter part + a RBS part as a instance, a promoter + a RBS Is usually registered as a composite part. What designers usually do is to enter part number they used in order and generate the new composite part with scars. Nevertheless, for the parts of XJTLU-CHINA, this operation is not applicable because if a promoter part + a RBS part was outputted as a new composite part with scars, there would be no scar remained at the end of RBS part. To fix this problem, we have to registry several spacer parts and put them into our composite parts in the manner of generating blunt end. false false _2250_ 25962 25962 9 false It part is unnecessary for the teams that use 3A assembly. false Wenbo Xu BBa_K1824889 1 BBa_K1824889 A Transcriptional Spacer Sequence 2015-09-05T11:00:00Z 2015-09-07T10:21:43Z E. coli promoter for tetracycline efflux protein gene Bacterial operator O2 for the tetR and tetA genes false false _2250_ 25962 25962 9 Not in stock false This is the second operator of pTet false Wenbo Xu BBa_B0034_sequence 1 aaagaggagaaa BBa_J64991_sequence 1 tccctatcagtgatagaga BBa_J23105_sequence 1 tttacggctagctcagtcctaggtactatgctagc BBa_K1824890_sequence 1 tactag BBa_K1824009_sequence 1 tttacggctagctcagtcctaggtactatgctagctccctatcagtgatagagatactagagaaagaggagaaatactag BBa_K1824889_sequence 1 tactagag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z