BBa_K1824108
type: ComponentDefinition
persistent identity: BBa K1824108
display id: BBa_K1824108
version: 1
was generated by: iGEM to SBOL conversion
description: promoter+rbs+amajLime+ter
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T12:45:52Z
mutable provenance: NO
owned by: james
owned by: myers
mutable description: Composed of promoter+rbs+amajLime-rrnB terminator-T7 terminater, canbe used in further assembly.
discontinued: false
dominant: false
group u list: _2250_
m user id: 24766
owner id: 24766
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: NO
star: false
creator: Zhe Yang
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1824106
component: BBa_K1824893
sequence annotation: BBa_K1824893
sequence annotation: BBa_K1824106
sequence: https://synbiohub.org/public/igem/BBa_K1824108_sequence/1

BBa_K1824106
type: Component
persistent identity: component2456611
display id: component2456611
version: 1
top level: BBa_K1824108
definition: BBa_K1824106
access: public

BBa_K1824893
type: Component
persistent identity: component2456601
display id: component2456601
version: 1
top level: BBa_K1824108
definition: BBa_K1824893
access: public

BBa_K1824893
type: SequenceAnnotation
persistent identity: annotation2456601
display id: annotation2456601
version: 1
top level: BBa_K1824108
location: https://synbiohub.org/public/igem/BBa_K1824108/annotation2456601/range2456601/1
component: BBa_K1824893

https://synbiohub.org/public/igem/BBa_K1824108/annotation2456601/range2456601/1
type: Range
persistent identity: range2456601
display id: range2456601
version: 1
top level: BBa_K1824108
direction: direction/reverse
start: 1
end: 61
orientation: inline

BBa_K1824106
type: SequenceAnnotation
persistent identity: annotation2456611
display id: annotation2456611
version: 1
top level: BBa_K1824108
location: https://synbiohub.org/public/igem/BBa_K1824108/annotation2456611/range2456611/1
component: BBa_K1824106

https://synbiohub.org/public/igem/BBa_K1824108/annotation2456611/range2456611/1
type: Range
persistent identity: range2456611
display id: range2456611
version: 1
top level: BBa_K1824108
direction: direction/reverse
start: 68
end: 913
orientation: inline

BBa_K1824106
type: ComponentDefinition
persistent identity: BBa K1824106
display id: BBa_K1824106
version: 1
was generated by: iGEM to SBOL conversion
description: amajLime+terminator
created: 2015-09-14T11:00:00Z
modified: 2015-09-15T12:38:12Z
mutable provenance: NO
owned by: james
owned by: myers
mutable description: Composed of amajLime-rrnB terminator-T7 terminater, canbe used in further assembly.
discontinued: false
dominant: false
group u list: _2250_
m user id: 25818
owner id: 24766
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: NO
star: false
creator: Zhe Yang
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1824889
component: BBa_K1033916
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_K1033916
sequence annotation: BBa_K1824889
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K1824106_sequence/1

BBa_K1824889
type: Component
persistent identity: component2456426
display id: component2456426
version: 1
top level: BBa_K1824106
definition: BBa_K1824889
access: public

BBa_K1033916
type: Component
persistent identity: component2456425
display id: component2456425
version: 1
top level: BBa_K1824106
definition: BBa_K1033916
access: public

BBa_B0012
type: Component
persistent identity: component2456429
display id: component2456429
version: 1
top level: BBa_K1824106
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2456427
display id: component2456427
version: 1
top level: BBa_K1824106
definition: BBa_B0010
access: public

BBa_K1033916
type: SequenceAnnotation
persistent identity: annotation2456425
display id: annotation2456425
version: 1
top level: BBa_K1824106
location: https://synbiohub.org/public/igem/BBa_K1824106/annotation2456425/range2456425/1
component: BBa_K1033916

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type: Range
persistent identity: range2456425
display id: range2456425
version: 1
top level: BBa_K1824106
direction: direction/reverse
start: 1
end: 693
orientation: inline

BBa_K1824889
type: SequenceAnnotation
persistent identity: annotation2456426
display id: annotation2456426
version: 1
top level: BBa_K1824106
location: https://synbiohub.org/public/igem/BBa_K1824106/annotation2456426/range2456426/1
component: BBa_K1824889

https://synbiohub.org/public/igem/BBa_K1824106/annotation2456426/range2456426/1
type: Range
persistent identity: range2456426
display id: range2456426
version: 1
top level: BBa_K1824106
direction: direction/reverse
start: 702
end: 709
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2456427
display id: annotation2456427
version: 1
top level: BBa_K1824106
location: https://synbiohub.org/public/igem/BBa_K1824106/annotation2456427/range2456427/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1824106/annotation2456427/range2456427/1
type: Range
persistent identity: range2456427
display id: range2456427
version: 1
top level: BBa_K1824106
direction: direction/forward
start: 718
end: 797
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2456429
display id: annotation2456429
version: 1
top level: BBa_K1824106
location: https://synbiohub.org/public/igem/BBa_K1824106/annotation2456429/range2456429/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1824106/annotation2456429/range2456429/1
type: Range
persistent identity: range2456429
display id: range2456429
version: 1
top level: BBa_K1824106
direction: direction/forward
start: 806
end: 846
orientation: inline

BBa_K1824893
type: ComponentDefinition
persistent identity: BBa K1824893
display id: BBa_K1824893
version: 1
was generated by: iGEM to SBOL conversion
description: a strong promoter plus a strong RBS
created: 2015-09-09T11:00:00Z
modified: 2015-09-11T08:37:07Z
mutable provenance: J23119 is from a constitutive promoter family and B0034 is based on Elowitz repressilator.
owned by: james
owned by: myers
mutable description: A regulatory part composed of a strong promoter and a strong RBS, which can be used in both transcription and translation. Just add any protein after it.
discontinued: false
dominant: false
group u list: _2250_
m user id: 26000
owner id: 26000
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No special concern
star: false
creator: Xinhao Wang
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1824890
component: BBa_B0034
component: BBa_K1824889
component: BBa_J23119
sequence annotation: BBa_J23119
sequence annotation: BBa_K1824889
sequence annotation: BBa_B0034
sequence annotation: BBa_K1824890
sequence: https://synbiohub.org/public/igem/BBa_K1824893_sequence/1

BBa_K1824890
type: Component
persistent identity: component2450243
display id: component2450243
version: 1
top level: BBa_K1824893
definition: BBa_K1824890
access: public

BBa_B0034
type: Component
persistent identity: component2450242
display id: component2450242
version: 1
top level: BBa_K1824893
definition: BBa_B0034
access: public

BBa_K1824889
type: Component
persistent identity: component2450240
display id: component2450240
version: 1
top level: BBa_K1824893
definition: BBa_K1824889
access: public

BBa_J23119
type: Component
persistent identity: component2450239
display id: component2450239
version: 1
top level: BBa_K1824893
definition: BBa_J23119
access: public

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2450239
display id: annotation2450239
version: 1
top level: BBa_K1824893
location: https://synbiohub.org/public/igem/BBa_K1824893/annotation2450239/range2450239/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K1824893/annotation2450239/range2450239/1
type: Range
persistent identity: range2450239
display id: range2450239
version: 1
top level: BBa_K1824893
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K1824889
type: SequenceAnnotation
persistent identity: annotation2450240
display id: annotation2450240
version: 1
top level: BBa_K1824893
location: https://synbiohub.org/public/igem/BBa_K1824893/annotation2450240/range2450240/1
component: BBa_K1824889

https://synbiohub.org/public/igem/BBa_K1824893/annotation2450240/range2450240/1
type: Range
persistent identity: range2450240
display id: range2450240
version: 1
top level: BBa_K1824893
direction: direction/reverse
start: 36
end: 43
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2450242
display id: annotation2450242
version: 1
top level: BBa_K1824893
location: https://synbiohub.org/public/igem/BBa_K1824893/annotation2450242/range2450242/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1824893/annotation2450242/range2450242/1
type: Range
persistent identity: range2450242
display id: range2450242
version: 1
top level: BBa_K1824893
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1824890
type: SequenceAnnotation
persistent identity: annotation2450243
display id: annotation2450243
version: 1
top level: BBa_K1824893
location: https://synbiohub.org/public/igem/BBa_K1824893/annotation2450243/range2450243/1
component: BBa_K1824890

https://synbiohub.org/public/igem/BBa_K1824893/annotation2450243/range2450243/1
type: Range
persistent identity: range2450243
display id: range2450243
version: 1
top level: BBa_K1824893
direction: direction/reverse
start: 56
end: 61
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_K1033916
type: ComponentDefinition
persistent identity: BBa K1033916
display id: BBa_K1033916
version: 1
was generated by: iGEM to SBOL conversion
description: amajLime, yellow-green chromoprotein
created: 2013-08-24T11:00:00Z
modified: 2015-05-08T01:08:49Z
mutable provenance: The anemone Anemonia majano.
owned by: james
owned by: myers
mutable description: This chromoprotein from the coral Anemonia majano, amajLime, naturally exhibits strong color when expressed.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Synthesized and codon optimized for E coli by GenScript.
star: false
creator: Sabri Jamal
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: amajLime
sequence: https://synbiohub.org/public/igem/BBa_K1033916_sequence/1

amajLime
type: SequenceAnnotation
persistent identity: annotation2332103
display id: annotation2332103
version: 1
top level: BBa_K1033916
location: https://synbiohub.org/public/igem/BBa_K1033916/annotation2332103/range2332103/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1033916/annotation2332103/range2332103/1
type: Range
persistent identity: range2332103
display id: range2332103
version: 1
top level: BBa_K1033916
direction: direction/forward
start: 1
end: 690
orientation: inline

BBa_K1824889
type: ComponentDefinition
persistent identity: BBa K1824889
display id: BBa_K1824889
version: 1
was generated by: iGEM to SBOL conversion
description: A Transcriptional Spacer Sequence
created: 2015-09-05T11:00:00Z
modified: 2015-09-07T10:21:43Z
mutable provenance: E. coli promoter for tetracycline efflux protein gene
owned by: james
owned by: myers
mutable description: Bacterial operator O2 for the tetR and tetA genes
discontinued: false
dominant: false
experience: experience/None
group u list: _2250_
m user id: 25962
owner id: 25962
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is the second operator of pTet
star: false
creator: Wenbo Xu
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K1824889_sequence/1

BBa_K1824890
type: ComponentDefinition
persistent identity: BBa K1824890
display id: BBa_K1824890
version: 1
was generated by: iGEM to SBOL conversion
description: The Sequence of the SpeI/XbaI scar in BioBricks standard assembly.
created: 2015-09-06T11:00:00Z
modified: 2015-09-07T10:05:29Z
mutable provenance: It is the scar sequence of 3A assembly.
owned by: james
owned by: myers
mutable description: In most cases, designers usually tend to embedded an appropriate spacer sequence between one individual part and the other. The 3A assembly, a commonly used manipulation method, creating scar between biobricks and, this unique scar usually served as default transcription spacer sequence. XJTLU-CHINA 2015 used Gibson assembly instead of conventional 3A assembly to create complex parts. If the reader have any interest to read the assembly diagram that the team promoted, they would find out that Gibson assembly was the ideal choices for the project. Though XJTLU-CHINA used Gibson assembly, which means parts could directly attach to one another without scars, the team still persevered the usually used scar sequence as the spacer. If readers looked through the assembly diagram that the team provided, they would find out that, for the constructs like a promoter part + a RBS part, the team not only embedded the spacer between the promoter and the RBS but also attach a spacer at the end of the RBS part. If the readers know the mechanism of Gibson assembly, they would know that it is for the one of the greatest feature of Gibson assembly, scarless manipulation. However, this design became problematical when the team registry the parts. Take the mentioned a promoter part + a RBS part as a instance, a promoter + a RBS Is usually registered as a composite part. What designers usually do is to enter part number they used in order and generate the new composite part with scars. Nevertheless, for the parts of XJTLU-CHINA, this operation is not applicable because if a promoter part + a RBS part was outputted as a new composite part with scars, there would be no scar remained at the end of RBS part. To fix this problem, we have to registry several spacer parts and put them into our composite parts in the manner of generating blunt end.
discontinued: false
dominant: false
experience: experience/None
group u list: _2250_
m user id: 25962
owner id: 25962
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: It part is unnecessary for the teams that use 3A assembly.
star: false
creator: Wenbo Xu
type: DnaRegion
role: partType/Scar
role: SO:0001953
sequence: https://synbiohub.org/public/igem/BBa_K1824890_sequence/1

https://synbiohub.org/public/igem/BBa_K1824108_sequence/1
type: Sequence
persistent identity: BBa K1824108 sequence
display id: BBa_K1824108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagtactagatggcactgagcaacaagtttatcggtgatgatatgaaaatgacgtaccacatggacggctgcgtgaacggccactactttacggtgaaaggtgaaggcaacggtaaaccgtatgaaggcacccagacgagcacctttaaagttacgatggcgaatggcggtccgctggcctttagtttcgatattctgtccaccgtctttaaatacggcaaccgttgcttcacggcgtatccgaccagcatgccggattactttaagcaagccttcccggacggcatgtcttatgaacgcacgtttacctacgaagatggcggtgtggcgaccgccagctgggaaatttctctgaaaggcaactgtttcgaacataagagtacctttcacggcgtgaatttcccggcagatggtccggttatggctaaaaagaccacgggttgggacccgtcatttgaaaaaatgacggtctgcgatggcatcctgaagggtgacgtgaccgcatttctgatgctgcaaggcggcggcaattatcgttgtcaattccatacgtcctacaaaaccaaaaagccggttacgatgccgccgaaccatgtggttgaacaccgtattgcgcgcaccgatctggacaaaggtggcaattcagttcagctgacggaacatgcagtcgctcacatcacctcggtcgtgccgttctaataatactagagtactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1824106_sequence/1
type: Sequence
persistent identity: BBa K1824106 sequence
display id: BBa_K1824106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcactgagcaacaagtttatcggtgatgatatgaaaatgacgtaccacatggacggctgcgtgaacggccactactttacggtgaaaggtgaaggcaacggtaaaccgtatgaaggcacccagacgagcacctttaaagttacgatggcgaatggcggtccgctggcctttagtttcgatattctgtccaccgtctttaaatacggcaaccgttgcttcacggcgtatccgaccagcatgccggattactttaagcaagccttcccggacggcatgtcttatgaacgcacgtttacctacgaagatggcggtgtggcgaccgccagctgggaaatttctctgaaaggcaactgtttcgaacataagagtacctttcacggcgtgaatttcccggcagatggtccggttatggctaaaaagaccacgggttgggacccgtcatttgaaaaaatgacggtctgcgatggcatcctgaagggtgacgtgaccgcatttctgatgctgcaaggcggcggcaattatcgttgtcaattccatacgtcctacaaaaccaaaaagccggttacgatgccgccgaaccatgtggttgaacaccgtattgcgcgcaccgatctggacaaaggtggcaattcagttcagctgacggaacatgcagtcgctcacatcacctcggtcgtgccgttctaataatactagagtactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1824893_sequence/1
type: Sequence
persistent identity: BBa K1824893 sequence
display id: BBa_K1824893_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1033916_sequence/1
type: Sequence
persistent identity: BBa K1033916 sequence
display id: BBa_K1033916_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcactgagcaacaagtttatcggtgatgatatgaaaatgacgtaccacatggacggctgcgtgaacggccactactttacggtgaaaggtgaaggcaacggtaaaccgtatgaaggcacccagacgagcacctttaaagttacgatggcgaatggcggtccgctggcctttagtttcgatattctgtccaccgtctttaaatacggcaaccgttgcttcacggcgtatccgaccagcatgccggattactttaagcaagccttcccggacggcatgtcttatgaacgcacgtttacctacgaagatggcggtgtggcgaccgccagctgggaaatttctctgaaaggcaactgtttcgaacataagagtacctttcacggcgtgaatttcccggcagatggtccggttatggctaaaaagaccacgggttgggacccgtcatttgaaaaaatgacggtctgcgatggcatcctgaagggtgacgtgaccgcatttctgatgctgcaaggcggcggcaattatcgttgtcaattccatacgtcctacaaaaccaaaaagccggttacgatgccgccgaaccatgtggttgaacaccgtattgcgcgcaccgatctggacaaaggtggcaattcagttcagctgacggaacatgcagtcgctcacatcacctcggtcgtgccgttctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1824889_sequence/1
type: Sequence
persistent identity: BBa K1824889 sequence
display id: BBa_K1824889_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1824890_sequence/1
type: Sequence
persistent identity: BBa K1824890 sequence
display id: BBa_K1824890_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z