BBa_K1824557
1
BBa_K1824557
RNA Thermometer A1 (Specially designed for pBAD)
2015-09-04T11:00:00Z
2015-09-05T08:51:07Z
FourU was previously submitted by TuDelft 2008.
This is a specially designed A1 RNA thermometer that with a unique spacer at the front, which would make it compatible with pBAD <partinfo>BBa_I13453</partinfo>.
A1 RNA thermometer have the hairpin structure that harbors the Shine-Dalgarno sequence (SD sequence) and, in this way, make it inaccessible to the 30S unit of the bacterial ribosome, resulting in translational inactivation (Figure 2). The melting temperature of this RNA thermometer is 42 Celsius degree. Once reaching the melting temperature, hairpin structure would vanish and as a result, exposing the SD sequence to trigger the translation process.
Different promoters have their own transcription start sites and, in most cases, + 1 sites are embedded in promoter sequence. Hence, it is normal that transcripted RNA usually carry part of promoter sequence. However, for regulatory parts like RNA thermometer, truncation or alteration of the RNA sequence could be destructive. Hence, special designed RNA spacer between transcripted part of promoters and RNA thermometers are important for maintaining the secondary structure of RNA thermometer.
For pBAD <partinfo>BBa_I13453</partinfo>, transcription starts at TCTCCAT'''A''' (transcription start site indicated in bold). Based on this, BBa_K1824557 was specially designed with a spacer that had less probability to interact with the functional structure of RNA thermometer.
The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). For testing results of pBAD-A1, See <partinfo>BBa_K1824003</partinfo>.
false
false
_2250_
25962
25962
9
false
To maintain the secondary structure of RNA thermometers, researchers need to be careful with of the use of any parts that couple with it. For instance, in some cases, part of operator region can be transcribed into RNAs and form tight secondary structure and thus interfere RNA thermometers.
false
Wenbo Xu
BBa_K1824557_sequence
1
tactagagtactagagctctttaaaaaaaaaaagtactaaggagtactag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z