BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_K1824896
1
BBa_K1824896
J23100 + RBS
2015-09-10T11:00:00Z
2015-09-11T09:57:14Z
Parts J23100 through J23119 are a family of constitutive promoter parts isolated from a small combinatorial library. B0030 is a strong RBS based on Ron Weiss thesis
Constitutive promoter J23100 plus RBS.
false
false
_2250_
26000
26000
9
false
no special concern
false
Xinhao Wang
component2452341
1
BBa_K1824889
component2452343
1
BBa_B0030
component2452340
1
BBa_J23100
component2452345
1
BBa_K1824890
annotation2452340
1
BBa_J23100
range2452340
1
1
35
annotation2452341
1
BBa_K1824889
range2452341
1
44
51
annotation2452343
1
BBa_B0030
range2452343
1
60
74
annotation2452345
1
BBa_K1824890
range2452345
1
83
88
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K1824890
1
BBa_K1824890
The Sequence of the SpeI/XbaI scar in BioBricks standard assembly.
2015-09-06T11:00:00Z
2015-09-07T10:05:29Z
It is the scar sequence of 3A assembly.
In most cases, designers usually tend to embedded an appropriate spacer sequence between one individual part and the other. The 3A assembly, a commonly used manipulation method, creating scar between biobricks and, this unique scar usually served as default transcription spacer sequence.
XJTLU-CHINA 2015 used Gibson assembly instead of conventional 3A assembly to create complex parts. If the reader have any interest to read the assembly diagram that the team promoted, they would find out that Gibson assembly was the ideal choices for the project.
Though XJTLU-CHINA used Gibson assembly, which means parts could directly attach to one another without scars, the team still persevered the usually used scar sequence as the spacer. If readers looked through the assembly diagram that the team provided, they would find out that, for the constructs like a promoter part + a RBS part, the team not only embedded the spacer between the promoter and the RBS but also attach a spacer at the end of the RBS part. If the readers know the mechanism of Gibson assembly, they would know that it is for the one of the greatest feature of Gibson assembly, scarless manipulation.
However, this design became problematical when the team registry the parts. Take the mentioned a promoter part + a RBS part as a instance, a promoter + a RBS Is usually registered as a composite part. What designers usually do is to enter part number they used in order and generate the new composite part with scars. Nevertheless, for the parts of XJTLU-CHINA, this operation is not applicable because if a promoter part + a RBS part was outputted as a new composite part with scars, there would be no scar remained at the end of RBS part.
To fix this problem, we have to registry several spacer parts and put them into our composite parts in the manner of generating blunt end.
false
false
_2250_
25962
25962
9
false
It part is unnecessary for the teams that use 3A assembly.
false
Wenbo Xu
BBa_K1824889
1
BBa_K1824889
A Transcriptional Spacer Sequence
2015-09-05T11:00:00Z
2015-09-07T10:21:43Z
E. coli promoter for tetracycline efflux protein gene
Bacterial operator O2 for the tetR and tetA genes
false
false
_2250_
25962
25962
9
Not in stock
false
This is the second operator of pTet
false
Wenbo Xu
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_K1824890_sequence
1
tactag
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1824889_sequence
1
tactagag
BBa_K1824896_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagtactagagtactagagattaaagaggagaaatactagagtactag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z