BBa_K1826005
1
RNAcj
RNA thermometer + cjBlue
2016-10-04T11:00:00Z
2016-10-05T06:55:11Z
The cjBlue , green chromoprotein is from the Cnidopus japonicus sea anemone. RNA thermometer is from Bradyrhizobium japonicum that initiates translation at 42??C.
This part is a composite of RNA thermometer and cjBlue, green chromoprotein. The RNA thermometer is an untranslated RNA sequence that will allow translation only above 42??C. Below this temperature, the RNA thermometer sequence will fold into a complex structure and prevent translation. The cjBlue , green chromoprotein is from the Cnidopus japonicus sea anemone. It naturally exhibits dark green color when expressed. The cjBlue protein is readily expressed in E.coli. The cjBlue acts as a reporter gene and the RNA thermometer, as a temperature controlled switch.
true
false
_2252_
27883
27883
9
true
Since the RNA thermometer sequence was too small, we had to keep it in its backbone and ligate cjBlue to it.
false
Saishreyas Gourishankar Iyer
component2486017
1
BBa_K592011
component2486015
1
BBa_K115001
annotation2486015
1
BBa_K115001
range2486015
1
1
96
annotation2486017
1
BBa_K592011
range2486017
1
103
804
BBa_K592011
1
cjBlue
cjBlue, green chromoprotein
2011-09-17T11:00:00Z
2015-05-08T01:12:49Z
Cnidopus japonicus
This chromoprotein, cjBlue, naturally exhibits strong green/blue color when expressed. Compared to amilCP(BBa_K592009) and amilGFP(BBa_K592010), the color development is slower. On agar plates and in liquid culture, the color is readily visible to naked eye after 24-48 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation.
false
false
_763_
0
7929
9
In stock
false
Codon-optimization for expression in E.coli necessary.
false
Antonio Ascue Avalos
annotation2131787
1
cjBlue
range2131787
1
1
699
BBa_K115001
1
BBa_K115001
RNA thermometer (ROSE)
2008-07-14T11:00:00Z
2015-05-08T01:09:27Z
This sequence is taken from the Bradirhizobium Japonicum (BA000040.) as the 5'UTR (ROSE) of a heat shock protein (prfA).
Released HQ 2013
This part is designed as a temperature inducible RBS. Only designed so far.
false
false
_223_
0
3007
9
In stock
true
The secondary structure is important to the function of these regions, but part of the wt secondary structure is destroyed by the scar. We've tried to alter the sequence so the predicted structure (through mfold and those kind of servers) is sort of conserved, but temperature sensitivity still has to be tested. If it doesn't work, possible solution might be the addition of a larger conserved part of the wt, which implies a small part of wt protein sequence as well.
true
O.M.J.A. Stassen
annotation1966927
1
Predicted Stem Loop
range1966927
1
16
36
annotation1966928
1
Predicted Stem Loop
range1966928
1
39
71
annotation1966929
1
Predicted Stem Loop extending to startcodon
range1966929
1
72
96
annotation1966926
1
Predicted Stem Loop
range1966926
1
1
15
annotation1966896
1
SD
range1966896
1
90
95
annotation1966898
1
Biobrick-Scar-adaptation
range1966898
1
74
79
BBa_K115001_sequence
1
gccgcgacaagcggtccgggcgccctaggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtcaagttcttgctccttggaggat
BBa_K592011_sequence
1
atggcttccaaaataagcgacaacgtacgtatcaaactgtatatggagggcacggttaataatcaccacttcatgtgtgaagcggagggtgagggcaagccatacgaaggaacgcagatggaaaacattaaagtgaccaaaggaggcccgctgccgttctcttttgatatcctgacgccgaactgccaatatggttctgtagccataaccaagtacacgtcggggattccggactattttaaacagtcattccctgaaggttttacctgggaaagaaccaccatttatgaagatggggcttatctgacaactcagcaggaaaccaaacttgatggaaattgcttagtctacaatattaaaatcctcggctgcaattttccccccaatggtcctgttatgcagaaaaaaacgcaaggctgggaaccatgttgcgagatgcgctatacacgtgatggtgtcttgtgcggtcagacattaatggcactgaaatgtgccgatgggaaccatctgacttgtcatctgcggactacttaccgatccaaaaaggcagcgaaggcgttgcaaatgccacctttccatttttcagaccatcgtccggaaattgtgaaggttagcgagaacggcacactgtttgagcagcacgaaagtagtgtggcacgctattgtcagacatgcccgagcaaacttggtcataattaataa
BBa_K1826005_sequence
1
gccgcgacaagcggtccgggcgccctaggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtcaagttcttgctccttggaggattactagatggcttccaaaataagcgacaacgtacgtatcaaactgtatatggagggcacggttaataatcaccacttcatgtgtgaagcggagggtgagggcaagccatacgaaggaacgcagatggaaaacattaaagtgaccaaaggaggcccgctgccgttctcttttgatatcctgacgccgaactgccaatatggttctgtagccataaccaagtacacgtcggggattccggactattttaaacagtcattccctgaaggttttacctgggaaagaaccaccatttatgaagatggggcttatctgacaactcagcaggaaaccaaacttgatggaaattgcttagtctacaatattaaaatcctcggctgcaattttccccccaatggtcctgttatgcagaaaaaaacgcaaggctgggaaccatgttgcgagatgcgctatacacgtgatggtgtcttgtgcggtcagacattaatggcactgaaatgtgccgatgggaaccatctgacttgtcatctgcggactacttaccgatccaaaaaggcagcgaaggcgttgcaaatgccacctttccatttttcagaccatcgtccggaaattgtgaaggttagcgagaacggcacactgtttgagcagcacgaaagtagtgtggcacgctattgtcagacatgcccgagcaaacttggtcataattaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z