BBa_K1828991 1 BBa_K1828991 sgOri 2015-09-02T11:00:00Z 2015-09-03T10:28:34Z The target sequence for sgRNA comes from pMSP3535 plasmid sequence This part is the sgRNA designed for targeting origin of replication of pMSP3535. pMSP3535 is used for expression of genes in gram positive bacteria such as Streptococcus mutans using NICE expression system. pMSP3535 was kindly provided to Nazarbayev University by Dr.Gary Dunny' Lab, University of Minnesota false false _2254_ 20031 20031 9 false The design of sgRNA was done by using Nature protocols false Altynay Abdirakhmanova BBa_K1828991_sequence 1 aataggttgtttaattaactgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z