LOCUS BBa_K1830000 3837 bp DNA linear 25-Nov-2024 DEFINITION pET15BB ACCESSION BBa_K1830000 VERSION BBa_K1830000.1 FEATURES Location/Qualifiers promoter 1..20 /label=T7 misc_feature 20..42 /label=LacO misc_feature 88..90 /label=ATG misc_feature 100..117 /label=6X His tag misc_feature 127..144 /label=Thrombin misc_feature 151..156 /label=NgoMIV misc_feature 213..218 /label=SpeI stem_loop 241..293 /label=T7 CDS 2327..3529 /label=LacI gene misc_feature 291..2033 /label=pSB1C3 backbone CDS 1435..2092 /label=CAT gene misc_feature 241..243 /label=TAG misc_feature 145..150 /label=NdeI misc_feature 162..167 /label=BamHI ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gagataattt 61 tgttcaactt caagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc 121 agcggcctgg tgccgcgcgg cagccatatg gccggcctcg aggatccggc tgctaacaaa 181 gcccgaaagg aagctgagtt ggctgctgcc aaactagtct gcactccgct gagcaatacc 241 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg cagtccggca 301 aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc 361 gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 481 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 541 gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 961 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 1021 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 1081 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 1141 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 1201 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 1261 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc 1321 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct 1381 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc 1441 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc 1501 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt 1561 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa 1621 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa 1681 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg 1741 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg 1801 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt 1861 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc 1921 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg 1981 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg 2041 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc 2101 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt 2161 atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag 2221 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca 2281 gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattgggtg cctaatgagt 2341 gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 2401 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 2461 ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct 2521 ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct 2581 gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca 2641 ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca 2701 gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt 2761 gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct 2821 gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag 2881 aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca 2941 cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag 3001 agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct 3061 ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca 3121 ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac 3181 ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca 3241 gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc 3301 ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag 3361 aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact 3421 ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg 3481 ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcg aattcctcga 3541 cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg 3601 agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc 3661 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc 3721 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg 3781 gtgataccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat //