##gff-version 3
##sequence-region BBa_K1830000 1 3529
BBa_K1830000	.	tag	100	117	.	+	0	ID=annotation2475197;Name=6X His tag
BBa_K1830000	.	tag	127	144	.	+	0	ID=annotation2475198;Name=Thrombin
BBa_K1830000	.	stem_loop	241	293	.	+	0	ID=annotation2475204;Name=T7
BBa_K1830000	.	engineered_region	151	156	.	+	0	ID=annotation2475200;Name=NgoMIV
BBa_K1830000	.	engineered_region	213	218	.	+	0	ID=annotation2475202;Name=SpeI
BBa_K1830000	.	promoter	1	20	.	+	0	ID=annotation2475192;Name=T7
BBa_K1830000	.	start_codon	88	90	.	+	0	ID=annotation2475195;Name=ATG
BBa_K1830000	.	CDS	2327	3529	.	+	0	ID=annotation2475206;Name=LacI gene
BBa_K1830000	.	CDS	1435	2092	.	+	0	ID=annotation2475208;Name=CAT gene
BBa_K1830000	.	sequence_feature	162	167	.	+	0	ID=annotation2475211;Name=BamHI
BBa_K1830000	.	sequence_feature	145	150	.	+	0	ID=annotation2475210;Name=NdeI
BBa_K1830000	.	sequence_feature	291	2033	.	+	0	ID=annotation2475207;Name=pSB1C3 backbone
BBa_K1830000	.	non_covalent_binding_site	20	42	.	+	0	ID=annotation2475194;Name=LacO
BBa_K1830000	.	stop_codon	241	243	.	+	0	ID=annotation2475209;Name=TAG
>BBa_K1830000
taatacgactcactataggggaattgtgagcggataacaattcccctctagagataattttgttcaacttcaagaagga
gatataccatgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatggccggcct
cgaggatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaaactagtctgcactccgctgagcaat
acctagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgcagtccggcaaaaaagggcaaggtgt
caccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcg
ctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataac
gcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccaca
ggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagata
ccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgccttt
ctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagc
tgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggt
aagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagag
ttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttacct
tcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagca
gattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaac
tcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagtttta
aatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcaccaataaaaaacgcccggcg
gcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctc
gatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagcca
tcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtga
aaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagac
gaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatg
tgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgt
aacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccggatgagcattcatcag
gcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatcc
agctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggata
tatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaa
tacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatcaactcgagtgccacctga
cgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaa
tccttagctttcgctaaggatgatttctggaattgggtgcctaatgagtgagctaacttacattaattgcgttgcgctc
actgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttg
cgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctga
gagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatat
aacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggc
gcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatg
gtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatt
tatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaa
tgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagag
acatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaa
tgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccat
cgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggcc
agactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattca
gctccgccatcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggt
ctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctct
tccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtcgaattcctcgacgctctcccttatgc
gactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaagg
agatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtg
gcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgataccggcc
acgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaat