LOCUS BBa_K1830016 7116 bp DNA linear 23-Dec-2024 DEFINITION Dual docking extracellular scaffold ACCESSION BBa_K1830016 VERSION BBa_K1830016.1 FEATURES Location/Qualifiers promoter 1..173 /label=PnisA RBS 159..165 /label=RBS misc_feature 97..102 /label=-35 misc_feature 120..125 /label=-10 promoter 1..173 /label=BBa_K1830001 misc_feature 180..182 /label=ATG misc_feature 180..293 /label=Signal Peptide misc_feature 180..293 /label=BBa_K1830002 CDS 302..805 /label=CBD misc_feature 302..805 /label=BBa_K1830003 CDS 814..1344 /label=coh1C3 misc_feature 814..1344 /label=BBa_K1830004 CDS 1353..1844 /label=coh2S1 misc_feature 1353..1844 /label=BBa_K1830005 CDS 1853..2365 /label=CWA misc_feature 2270..2272 /label=TAA misc_feature 2165..2179 /label=LPXTG misc_feature 1853..2365 /label=BBa_K1830006 misc_feature 2374..2471 /label=t1 misc_feature 2476..2520 /label=t2 terminator 2374..2529 /label=BBa_K1830007 misc_feature 2538..7116 /label=Lactis Shuttle misc_feature 2538..7116 /label=BBa_K1830008 ORIGIN 1 gaaacattaa caaatctaaa acagtcttaa ttctatcttg agaaagtatt ggtaataata 61 ttattgtcga taacgcgagc ataataaacg gctctgatta aattctgaag tttgttagat 121 acaatgattt cgttcgaagg aactacaaaa taaattataa ggaggcactc aaatactaga 181 tgaaaaaaaa gattatctca gctattttaa tgtctacagt gatactttct gctgctgccc 241 cgttgtcagg tgtttacgct gacacaaact cagatattgc taaacaagat gcgtactaga 301 gccggcaaat acaccagtat caggcaattt gaaggttgag ttctacaaca gcaatccttc 361 agatactact aactcaatca atcctcagtt caaggttact aataccggaa gcagtgcaat 421 tgatttgtcc aaactcacat tgagatatta ttatacagta gacggacaga aagatcagac 481 cttctggtgt gaccatgctg caataatcgg cagtaacggc agctacaacg gaattacttc 541 aaatgtaaaa ggaacatttg taaaaatgag ttcctcaaca aataacgcag acacctacct 601 tgaaataagc tttacaggcg gaactcttga accgggtgca catgttcaga tacaaggtag 661 atttgcaaag aatgactgga gtaactatac acagtcaaat gactactcat tcaagtctgc 721 ttcacagttt gttgaatggg atcaggtaac agcatacttg aacggtgttc ttgtatgggg 781 taaagaaccc ggtggcagtg tagtatacta gagccatcaa cacagcctgt aacaacacca 841 cctgcaacaa caaaaccacc tgcaacaaca aaaccacctg caacaacaat accgccgtca 901 gatgatccga atgcaataaa gattaaggtg gacacagtaa atgcaaaacc gggagacaca 961 gtaaatatac ctgtaagatt cagtggtata ccatccaagg gaatagcaaa ctgtgacttt 1021 gtatacagct atgacccgaa tgtacttgag ataatagaga taaaaccggg agaattgata 1081 gttgacccga atcctgacaa gagctttgat actgctgtat atcctgacag aaagataata 1141 gtattcctgt ttgcagaaga cagcggaaca ggagcgtatg caataactaa agacggagta 1201 tttgctacga tagtagcgaa agtaaaatcc ggagcaccta acggactcag tgtaatcaaa 1261 tttgtagaag taggcggatt tgcgaacaat gaccttgtag aacagaggac acagttcttt 1321 gacggtggag taaatgttgg agattactag aggataaagc ctcgagcatt gagcttaagt 1381 ttgaccgcaa taagggagaa gttggagata tacttattgg taccgtaagg ataaacaata 1441 tcaagaattt cgcaggattt caggtaaaca ttgtatatga tccaaaagtc ttaatggctg 1501 ttgaccctga aacggggaaa gaatttactt cttcaacatt tccgccagga cgcactgtac 1561 tgaaaaacaa tgcttacggc ccaatacaga ttgcggacaa tgatccggaa aaagggatac 1621 tgaacttcgc gcttgcatat tcatatattg cgggatacaa agaaacagga gtagcggagg 1681 aaagcggcat aattgcgaaa attggattta aaatactcca gaaaaagagc actgccgtaa 1741 aattccagga tacattaagc atgcccggag ctatttcggg aacacagctg tttgactggg 1801 acggagaagt tattaccgga tatgaggtaa tacagccgga taagtactag aggctttaga 1861 agaagcaaac agcaaattag ctgctcttga aaaacttaac aaagagcttg aagaaagcaa 1921 gaaattaaca gaaaaagaaa aagctgagct acaagcaaaa cttgaagcag aagcaaaagc 1981 actcaaagaa caattagcga aacaagctga agaacttgca aaactaagag ctggaaaagc 2041 atcagactca caaacccctg atgcaaaacc aggaaacaaa gttgttccag gtaaaggtca 2101 agcaccacaa gcaggtacaa aacctaacca aaacaaagca ccaatgaagg aaactaagag 2161 acagttacca tcaacaggtg aaacagctaa cccattcttc acagcggcag cccttactgt 2221 tatggcaaca gctggagtag cagcagttgt aaaacgcaaa gaagaaaact aagctatcac 2281 tttgtaatac tgagtgaaca tcaagagaga accagtcggt tctctctttt atgtatagaa 2341 gaatgagatt aaggaggtca caaactacta gagaggcatc aaataaaacg aaaggctcag 2401 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg 2461 acaaatccgc cgctctagct aagcagaagg ccatcctgac ggatggcctt tttgcgtttc 2521 tacaaactct actagagaga atcgatacga ttttgaagtg gcaacagata aaaaaaagca 2581 gtttaaaatt gttgctgaac ttttaaaaca agcaaataca atcattgtcg caacagatag 2641 cgacagagaa ggcgaaaaca ttgcctggtc gatcattcat aaagcaaatg ccttttctaa 2701 agataaaacg tataaaagac tatggatcaa tagtttagaa aaagatgtga tccgtagcgg 2761 ttttcaaaat ttgcaaccag gaatgaatta ctatcccttt tatcaagaag cgcaaaagaa 2821 aaacgaaatg atacaccaat cagtgcaaaa aaagatataa tgggagataa gacggttcgt 2881 gttcgtgctg acttgcacca tatcataaaa atcgaaacag caaagaatgg cggaaacgta 2941 aaagaagtta tggaaataag acttagaagc aaacttaaga gtgtgttgat agtgcagtat 3001 cttaaaattt tgtataatag gaattgaagt taaattagat gctaaaaatt tgtaattaag 3061 aaggagtgat tacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa 3121 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat 3181 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc 3241 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg 3301 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa 3361 aattgttggg agtattcctt accatttaag cacacaaatt attaaaaaag tggtttttga 3421 aagccatgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga 3481 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa 3541 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac 3601 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa 3661 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa 3721 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa 3781 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa 3841 agttacacgt tactaaaggg aatgtagata aattattagg tatactactg acagcttcca 3901 aggagctaaa gaggtcccta gcgcttagaa tcgctttagg aaacacgatc cagtccaata 3961 atcgtcgata aaaacttttg aaaaaggttg gtgaaattac ctacttttgg aataatcaca 4021 aatcacaagt gattaatcac aaatcacaag tgattaatca cttgtttatt aagatattaa 4081 aagctataat ttaaataaag cgtgaatttt attacacaaa aagagggggg agaaacttgg 4141 aactagcatt tagagaaagc ttaaaaaaga tgagaggtac caaatcaaaa gaaaaattct 4201 cccaagaatt agaaatgagt agatcaaatt attcacgaat agaatcagga aaatcagatc 4261 caaccataaa aacactagaa caaattgcaa agttaactaa ctcaacgcta gtagtggatt 4321 taatcccaaa tgagccaaca gaaccagaac cagaaacaga atcagaacaa gtaacattgg 4381 atttagaaat ggaagaagaa aaaagcaatg acttcgtgtg aataatgcac gaaatcgttg 4441 cttatttttt tttaaaagcg gtatactaga tataacgaaa caacgaactg aatagaaacg 4501 aaaaaagagc catgacacat ttataaaatg tttgacgaca ttttataaat gcatagcccg 4561 ataagattgc caaaccaacg cttatcagtt agtcagatga actcttccct cgtaagaagt 4621 tatttaatta actttgtttg aagacggtat ataaccgtac tatcattata tagggaaatc 4681 agagagtttt caagtatcta agctactgaa tttaagaatt gttaagcaat caatcggaaa 4741 tcgtttgatt gctttttttg tattcattta tagaaggtgg agtttgtatg aatcatgatg 4801 aatgtaaaac ttatataaaa aatagtttat tggagataag aaaattagca aatatctata 4861 cactagaaac gtttaagaaa gagttagaaa agagaaatat ctacttagaa acaaaatcag 4921 ataagtattt ttcttcggag ggggaagatt atatatataa gttaatagaa aataacaaaa 4981 taatttattc gattagtgga aaaaaattga cttataaagg aaaaaaatct ttttcaaaac 5041 atgcaatatt gaaacagttg aatgaaaaag caaaccaagt taattaaaca acctatttta 5101 taggatttat aggaaaggag aacagctgaa tgaatatccc ttttgttgta gaaactgtgc 5161 ttcatgacgg cttgttaaag tacaaattta aaaatagtaa aattcgctca atcactacca 5221 agccaggtaa aagcaaaggg gctatttttg cgtatcgctc aaaatcaagc atgattggcg 5281 gtcgtggtgt tgttctgact tccgaggaag cgattcaaga aaatcaagat acatttacac 5341 attggacacc caacgtttat cgttatggaa cgtatgcaga cgaaaaccgt tcatacacga 5401 aaggacattc tgaaaacaat ttaagacaaa tcaatacctt ctttattgat tttgatattc 5461 acacggcaaa agaaactatt tcagcaagcg atattttaac aaccgctatt gatttaggtt 5521 ttatgcctac tatgattatc aaatctgata aaggttatca agcatatttt gttttagaaa 5581 cgccagtcta tgtgacttca aaatcagaat ttaaatctgt caaagcagcc aaaataattt 5641 cgcaaaatat ccgagaatat tttggaaagt ctttgccagt tgatctaacg tgtaatcatt 5701 ttggtattgc tcgcatacca agaacggaca atgtagaatt ttttgatcct aattaccgtt 5761 attctttcaa agaatggcaa gattggtctt tcaaacaaac agataataag ggctttactc 5821 gttcaagtct aacggtttta agcggtacag aaggcaaaaa acaagtagat gaaccctggt 5881 ttaatctctt attgcacgaa acgaaatttt caggagaaaa gggtttaata gggcgtaata 5941 acgtcatgtt taccctctct ttagcctact ttagttcagg ctattcaatc gaaacgtgcg 6001 aatataatat gtttgagttt aataatcgat tagatcaacc cttagaagaa aaagaagtaa 6061 tcaaaattgt tagaagtgcc tattcagaaa actatcaagg ggctaatagg gaatacatta 6121 ccattctttg caaagcttgg gtatcaagtg atttaaccag taaagattta tttgtccgtc 6181 aagggtggtt taaattcaag aaaaaaagaa gcgaacgtca acgtgttcat ttgtcagaat 6241 ggaaagaaga tttaatggct tatattagcg aaaaaagcga tgtatacaag ccttatttag 6301 tgacgaccaa aaaagagatt agagaagtgc taggcattcc tgaacggaca ttagataaat 6361 tgctgaaggt actgaaggcg aatcaggaaa ttttctttaa gattaaacca ggaagaaatg 6421 gtggcattca acttgctagt gttaaatcat tgttgctatc gatcattaaa gtaaaaaaag 6481 aagaaaaaga aagctatata aaggcgctga caaattcttt tgacttagag catacattca 6541 ttcaagagac tttaaacaag ctagcagaac gccctaaaac ggacacacaa ctcgatttgt 6601 ttagctatga tacaggctga aaataaaacc cgcactatgc cattacattt atatctatga 6661 tacgtgtttg ttttttcttt gctgtttagc gaatgattag cagaaatata cagagtaaga 6721 ttttaattaa ttattagggg gagaaggaga gagtagcccg aaaactttta gttggcttgg 6781 actgaacgaa gtgagggaaa ggctactaaa acgtcgaggg gcagtgagag cgaagcgaac 6841 acttgatttt ttaattttct atcttttata ggtcattaga gtatacttat ttgtcctata 6901 aactatttag cagcataata gatttattga ataggtcatt taagttgagc atattagagg 6961 aggaaaatct tggagaaata tttgaagaac ccgattacat ggattggatt agttcttgtg 7021 gttacgtggt ttttaactaa aagtagtgaa tttttgattt ttggtgtgtg tgtcttgttg 7081 ttagtatttg ctagtcaaag tgattaaata gacttc //