BBa_K1830004 1 BBa_K1830004 coh1C3 2015-08-05T11:00:00Z 2015-08-06T10:17:31Z GeneBank CipA third type I cohesin domain Type I cohesin domain false false _2256_ 27439 27439 9 false GCA to GCT mmutation to remove PstI cut site false Mathieu Husser annotation2434513 1 coh1C3 range2434513 1 1 531 BBa_K1830002 1 SPusp45 SPusp45 2015-08-05T11:00:00Z 2015-08-08T08:43:24Z GeneBank Nisin inducible promoter and ribosome binding site of the Lactococcal NisA gene false false _2256_ 27439 27439 9 false RCF10 Compatibility false Mathieu Husser annotation2434514 1 ATG range2434514 1 1 3 annotation2434515 1 Signal Peptide range2434515 1 1 114 BBa_K1830008 1 LS L. lactis Shuttle vector 2015-08-05T11:00:00Z 2015-09-18T08:29:09Z AW Lactococcus Lactis shuttle vector part false false _2256_ 27439 27439 9 false false Mathieu Husser annotation2434556 1 Lactis Shuttle range2434556 1 1 4579 BBa_K1830007 1 BBa_K1830007 T1t2 2015-08-05T11:00:00Z 2015-09-06T01:55:27Z GeneBank Bidirectional terminator true false _2256_ 27439 27439 9 false false Mathieu Husser annotation2434523 1 t2 range2434523 1 103 147 annotation2434522 1 t1 range2434522 1 1 98 BBa_K1830003 1 CBD CBD 2015-08-05T11:00:00Z 2015-08-08T08:42:08Z GeneBank CipA Cellulose Binding Domain false false _2256_ 27439 27439 9 false CCG to CCA mutation to remove AgeI cut site GAA to GAG mutation to remove EcoRI cut site false Mathieu Husser annotation2434512 1 CBD range2434512 1 1 504 BBa_K1830016 1 BBa_K1830016 Dual docking extracellular scaffold 2015-09-17T11:00:00Z 2015-09-18T09:33:54Z iGEM Concordia 2015 This part is the main construct cloned by the iGEM Concordia 2015 team. It is the entire DNA sequence that allows for the Induction, expression, targeting and attachment of an extracellular scaffold onto the extracellular membrane of the lactic acid bacteria ''Lactococcus Lactis''. This scaffold contains two different dockerin domain that allow for the specific harboring of cohesin-fused proteins. true false _2256_ 27439 27439 9 false false Mathieu Husser component2475336 1 BBa_K1830002 component2475346 1 BBa_K1830006 component2475342 1 BBa_K1830005 component2475349 1 BBa_K1830007 component2475333 1 BBa_K1830001 component2475351 1 BBa_K1830008 component2475338 1 BBa_K1830003 component2475340 1 BBa_K1830004 annotation2475346 1 BBa_K1830006 range2475346 1 1853 2365 annotation2475333 1 BBa_K1830001 range2475333 1 1 173 annotation2475351 1 BBa_K1830008 range2475351 1 2538 7116 annotation2475349 1 BBa_K1830007 range2475349 1 2374 2529 annotation2475336 1 BBa_K1830002 range2475336 1 180 293 annotation2475340 1 BBa_K1830004 range2475340 1 814 1344 annotation2475342 1 BBa_K1830005 range2475342 1 1353 1844 annotation2475338 1 BBa_K1830003 range2475338 1 302 805 BBa_K1830006 1 BBa_K1830006 CWAm6 2015-08-05T11:00:00Z 2015-08-06T10:26:22Z GeneBank m6 protein, Streptococcus Pyogenes Gram+ bacterial cell wall anchor false false _2256_ 27439 27439 9 false TAA stop codon false Mathieu Husser annotation2434521 1 TAA range2434521 1 418 420 annotation2434520 1 CWA range2434520 1 1 513 annotation2434557 1 LPXTG range2434557 1 313 327 BBa_K1830001 1 PnisA PnisA 2015-08-05T11:00:00Z 2015-08-07T11:50:06Z L. Lactis NisA operon Promoter and ribosome binding site of the Lactococcal nisin inducible operon. false false _2256_ 27439 27439 9 Not in stock false Small part false Mathieu Husser annotation2434537 1 PnisA range2434537 1 1 173 annotation2434540 1 -10 range2434540 1 120 125 annotation2434539 1 -35 range2434539 1 97 102 annotation2434538 1 RBS range2434538 1 159 165 BBa_K1830005 1 BBa_K1830005 coh2S1 2015-08-05T11:00:00Z 2015-08-06T10:22:09Z GeneBank SdbA first type II cohesin domain, L. Lactis Type II cohesin domain false false _2256_ 27439 27439 9 false false Mathieu Husser annotation2434516 1 coh2S1 range2434516 1 1 492 BBa_K1830005_sequence 1 gataaagcctcgagcattgagcttaagtttgaccgcaataagggagaagttggagatatacttattggtaccgtaaggataaacaatatcaagaatttcgcaggatttcaggtaaacattgtatatgatccaaaagtcttaatggctgttgaccctgaaacggggaaagaatttacttcttcaacatttccgccaggacgcactgtactgaaaaacaatgcttacggcccaatacagattgcggacaatgatccggaaaaagggatactgaacttcgcgcttgcatattcatatattgcgggatacaaagaaacaggagtagcggaggaaagcggcataattgcgaaaattggatttaaaatactccagaaaaagagcactgccgtaaaattccaggatacattaagcatgcccggagctatttcgggaacacagctgtttgactgggacggagaagttattaccggatatgaggtaatacagccggataag BBa_K1830003_sequence 1 ccggcaaatacaccagtatcaggcaatttgaaggttgagttctacaacagcaatccttcagatactactaactcaatcaatcctcagttcaaggttactaataccggaagcagtgcaattgatttgtccaaactcacattgagatattattatacagtagacggacagaaagatcagaccttctggtgtgaccatgctgcaataatcggcagtaacggcagctacaacggaattacttcaaatgtaaaaggaacatttgtaaaaatgagttcctcaacaaataacgcagacacctaccttgaaataagctttacaggcggaactcttgaaccgggtgcacatgttcagatacaaggtagatttgcaaagaatgactggagtaactatacacagtcaaatgactactcattcaagtctgcttcacagtttgttgaatgggatcaggtaacagcatacttgaacggtgttcttgtatggggtaaagaacccggtggcagtgtagta BBa_K1830002_sequence 1 atgaaaaaaaagattatctcagctattttaatgtctacagtgatactttctgctgctgccccgttgtcaggtgtttacgctgacacaaactcagatattgctaaacaagatgcg BBa_K1830001_sequence 1 gaaacattaacaaatctaaaacagtcttaattctatcttgagaaagtattggtaataatattattgtcgataacgcgagcataataaacggctctgattaaattctgaagtttgttagatacaatgatttcgttcgaaggaactacaaaataaattataaggaggcactcaaa BBa_K1830008_sequence 1 agaatcgatacgattttgaagtggcaacagataaaaaaaagcagtttaaaattgttgctgaacttttaaaacaagcaaatacaatcattgtcgcaacagatagcgacagagaaggcgaaaacattgcctggtcgatcattcataaagcaaatgccttttctaaagataaaacgtataaaagactatggatcaatagtttagaaaaagatgtgatccgtagcggttttcaaaatttgcaaccaggaatgaattactatcccttttatcaagaagcgcaaaagaaaaacgaaatgatacaccaatcagtgcaaaaaaagatataatgggagataagacggttcgtgttcgtgctgacttgcaccatatcataaaaatcgaaacagcaaagaatggcggaaacgtaaaagaagttatggaaataagacttagaagcaaacttaagagtgtgttgatagtgcagtatcttaaaattttgtataataggaattgaagttaaattagatgctaaaaatttgtaattaagaaggagtgattacatgaacaaaaatataaaatattctcaaaactttttaacgagtgaaaaagtactcaaccaaataataaaacaattgaatttaaaagaaaccgataccgtttacgaaattggaacaggtaaagggcatttaacgacgaaactggctaaaataagtaaacaggtaacgtctattgaattagacagtcatctattcaacttatcgtcagaaaaattaaaactgaatactcgtgtcactttaattcaccaagatattctacagtttcaattccctaacaaacagaggtataaaattgttgggagtattccttaccatttaagcacacaaattattaaaaaagtggtttttgaaagccatgcgtctgacatctatctgattgttgaagaaggattctacaagcgtaccttggatattcaccgaacactagggttgctcttgcacactcaagtctcgattcagcaattgcttaagctgccagcggaatgctttcatcctaaaccaaaagtaaacagtgtcttaataaaacttacccgccataccacagatgttccagataaatattggaagctatatacgtactttgtttcaaaatgggtcaatcgagaatatcgtcaactgtttactaaaaatcagtttcatcaagcaatgaaacacgccaaagtaaacaatttaagtaccgttacttatgagcaagtattgtctatttttaatagttatctattatttaacgggaggaaataattctatgagtcgcttttgtaaatttggaaagttacacgttactaaagggaatgtagataaattattaggtatactactgacagcttccaaggagctaaagaggtccctagcgcttagaatcgctttaggaaacacgatccagtccaataatcgtcgataaaaacttttgaaaaaggttggtgaaattacctacttttggaataatcacaaatcacaagtgattaatcacaaatcacaagtgattaatcacttgtttattaagatattaaaagctataatttaaataaagcgtgaattttattacacaaaaagaggggggagaaacttggaactagcatttagagaaagcttaaaaaagatgagaggtaccaaatcaaaagaaaaattctcccaagaattagaaatgagtagatcaaattattcacgaatagaatcaggaaaatcagatccaaccataaaaacactagaacaaattgcaaagttaactaactcaacgctagtagtggatttaatcccaaatgagccaacagaaccagaaccagaaacagaatcagaacaagtaacattggatttagaaatggaagaagaaaaaagcaatgacttcgtgtgaataatgcacgaaatcgttgcttatttttttttaaaagcggtatactagatataacgaaacaacgaactgaatagaaacgaaaaaagagccatgacacatttataaaatgtttgacgacattttataaatgcatagcccgataagattgccaaaccaacgcttatcagttagtcagatgaactcttccctcgtaagaagttatttaattaactttgtttgaagacggtatataaccgtactatcattatatagggaaatcagagagttttcaagtatctaagctactgaatttaagaattgttaagcaatcaatcggaaatcgtttgattgctttttttgtattcatttatagaaggtggagtttgtatgaatcatgatgaatgtaaaacttatataaaaaatagtttattggagataagaaaattagcaaatatctatacactagaaacgtttaagaaagagttagaaaagagaaatatctacttagaaacaaaatcagataagtatttttcttcggagggggaagattatatatataagttaatagaaaataacaaaataatttattcgattagtggaaaaaaattgacttataaaggaaaaaaatctttttcaaaacatgcaatattgaaacagttgaatgaaaaagcaaaccaagttaattaaacaacctattttataggatttataggaaaggagaacagctgaatgaatatcccttttgttgtagaaactgtgcttcatgacggcttgttaaagtacaaatttaaaaatagtaaaattcgctcaatcactaccaagccaggtaaaagcaaaggggctatttttgcgtatcgctcaaaatcaagcatgattggcggtcgtggtgttgttctgacttccgaggaagcgattcaagaaaatcaagatacatttacacattggacacccaacgtttatcgttatggaacgtatgcagacgaaaaccgttcatacacgaaaggacattctgaaaacaatttaagacaaatcaataccttctttattgattttgatattcacacggcaaaagaaactatttcagcaagcgatattttaacaaccgctattgatttaggttttatgcctactatgattatcaaatctgataaaggttatcaagcatattttgttttagaaacgccagtctatgtgacttcaaaatcagaatttaaatctgtcaaagcagccaaaataatttcgcaaaatatccgagaatattttggaaagtctttgccagttgatctaacgtgtaatcattttggtattgctcgcataccaagaacggacaatgtagaattttttgatcctaattaccgttattctttcaaagaatggcaagattggtctttcaaacaaacagataataagggctttactcgttcaagtctaacggttttaagcggtacagaaggcaaaaaacaagtagatgaaccctggtttaatctcttattgcacgaaacgaaattttcaggagaaaagggtttaatagggcgtaataacgtcatgtttaccctctctttagcctactttagttcaggctattcaatcgaaacgtgcgaatataatatgtttgagtttaataatcgattagatcaacccttagaagaaaaagaagtaatcaaaattgttagaagtgcctattcagaaaactatcaaggggctaatagggaatacattaccattctttgcaaagcttgggtatcaagtgatttaaccagtaaagatttatttgtccgtcaagggtggtttaaattcaagaaaaaaagaagcgaacgtcaacgtgttcatttgtcagaatggaaagaagatttaatggcttatattagcgaaaaaagcgatgtatacaagccttatttagtgacgaccaaaaaagagattagagaagtgctaggcattcctgaacggacattagataaattgctgaaggtactgaaggcgaatcaggaaattttctttaagattaaaccaggaagaaatggtggcattcaacttgctagtgttaaatcattgttgctatcgatcattaaagtaaaaaaagaagaaaaagaaagctatataaaggcgctgacaaattcttttgacttagagcatacattcattcaagagactttaaacaagctagcagaacgccctaaaacggacacacaactcgatttgtttagctatgatacaggctgaaaataaaacccgcactatgccattacatttatatctatgatacgtgtttgttttttctttgctgtttagcgaatgattagcagaaatatacagagtaagattttaattaattattagggggagaaggagagagtagcccgaaaacttttagttggcttggactgaacgaagtgagggaaaggctactaaaacgtcgaggggcagtgagagcgaagcgaacacttgattttttaattttctatcttttataggtcattagagtatacttatttgtcctataaactatttagcagcataatagatttattgaataggtcatttaagttgagcatattagaggaggaaaatcttggagaaatatttgaagaacccgattacatggattggattagttcttgtggttacgtggtttttaactaaaagtagtgaatttttgatttttggtgtgtgtgtcttgttgttagtatttgctagtcaaagtgattaaatagacttc BBa_K1830006_sequence 1 gctttagaagaagcaaacagcaaattagctgctcttgaaaaacttaacaaagagcttgaagaaagcaagaaattaacagaaaaagaaaaagctgagctacaagcaaaacttgaagcagaagcaaaagcactcaaagaacaattagcgaaacaagctgaagaacttgcaaaactaagagctggaaaagcatcagactcacaaacccctgatgcaaaaccaggaaacaaagttgttccaggtaaaggtcaagcaccacaagcaggtacaaaacctaaccaaaacaaagcaccaatgaaggaaactaagagacagttaccatcaacaggtgaaacagctaacccattcttcacagcggcagcccttactgttatggcaacagctggagtagcagcagttgtaaaacgcaaagaagaaaactaagctatcactttgtaatactgagtgaacatcaagagagaaccagtcggttctctcttttatgtatagaagaatgagattaaggaggtcacaaac BBa_K1830016_sequence 1 gaaacattaacaaatctaaaacagtcttaattctatcttgagaaagtattggtaataatattattgtcgataacgcgagcataataaacggctctgattaaattctgaagtttgttagatacaatgatttcgttcgaaggaactacaaaataaattataaggaggcactcaaatactagatgaaaaaaaagattatctcagctattttaatgtctacagtgatactttctgctgctgccccgttgtcaggtgtttacgctgacacaaactcagatattgctaaacaagatgcgtactagagccggcaaatacaccagtatcaggcaatttgaaggttgagttctacaacagcaatccttcagatactactaactcaatcaatcctcagttcaaggttactaataccggaagcagtgcaattgatttgtccaaactcacattgagatattattatacagtagacggacagaaagatcagaccttctggtgtgaccatgctgcaataatcggcagtaacggcagctacaacggaattacttcaaatgtaaaaggaacatttgtaaaaatgagttcctcaacaaataacgcagacacctaccttgaaataagctttacaggcggaactcttgaaccgggtgcacatgttcagatacaaggtagatttgcaaagaatgactggagtaactatacacagtcaaatgactactcattcaagtctgcttcacagtttgttgaatgggatcaggtaacagcatacttgaacggtgttcttgtatggggtaaagaacccggtggcagtgtagtatactagagccatcaacacagcctgtaacaacaccacctgcaacaacaaaaccacctgcaacaacaaaaccacctgcaacaacaataccgccgtcagatgatccgaatgcaataaagattaaggtggacacagtaaatgcaaaaccgggagacacagtaaatatacctgtaagattcagtggtataccatccaagggaatagcaaactgtgactttgtatacagctatgacccgaatgtacttgagataatagagataaaaccgggagaattgatagttgacccgaatcctgacaagagctttgatactgctgtatatcctgacagaaagataatagtattcctgtttgcagaagacagcggaacaggagcgtatgcaataactaaagacggagtatttgctacgatagtagcgaaagtaaaatccggagcacctaacggactcagtgtaatcaaatttgtagaagtaggcggatttgcgaacaatgaccttgtagaacagaggacacagttctttgacggtggagtaaatgttggagattactagaggataaagcctcgagcattgagcttaagtttgaccgcaataagggagaagttggagatatacttattggtaccgtaaggataaacaatatcaagaatttcgcaggatttcaggtaaacattgtatatgatccaaaagtcttaatggctgttgaccctgaaacggggaaagaatttacttcttcaacatttccgccaggacgcactgtactgaaaaacaatgcttacggcccaatacagattgcggacaatgatccggaaaaagggatactgaacttcgcgcttgcatattcatatattgcgggatacaaagaaacaggagtagcggaggaaagcggcataattgcgaaaattggatttaaaatactccagaaaaagagcactgccgtaaaattccaggatacattaagcatgcccggagctatttcgggaacacagctgtttgactgggacggagaagttattaccggatatgaggtaatacagccggataagtactagaggctttagaagaagcaaacagcaaattagctgctcttgaaaaacttaacaaagagcttgaagaaagcaagaaattaacagaaaaagaaaaagctgagctacaagcaaaacttgaagcagaagcaaaagcactcaaagaacaattagcgaaacaagctgaagaacttgcaaaactaagagctggaaaagcatcagactcacaaacccctgatgcaaaaccaggaaacaaagttgttccaggtaaaggtcaagcaccacaagcaggtacaaaacctaaccaaaacaaagcaccaatgaaggaaactaagagacagttaccatcaacaggtgaaacagctaacccattcttcacagcggcagcccttactgttatggcaacagctggagtagcagcagttgtaaaacgcaaagaagaaaactaagctatcactttgtaatactgagtgaacatcaagagagaaccagtcggttctctcttttatgtatagaagaatgagattaaggaggtcacaaactactagagaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgctctagctaagcagaaggccatcctgacggatggcctttttgcgtttctacaaactctactagagagaatcgatacgattttgaagtggcaacagataaaaaaaagcagtttaaaattgttgctgaacttttaaaacaagcaaatacaatcattgtcgcaacagatagcgacagagaaggcgaaaacattgcctggtcgatcattcataaagcaaatgccttttctaaagataaaacgtataaaagactatggatcaatagtttagaaaaagatgtgatccgtagcggttttcaaaatttgcaaccaggaatgaattactatcccttttatcaagaagcgcaaaagaaaaacgaaatgatacaccaatcagtgcaaaaaaagatataatgggagataagacggttcgtgttcgtgctgacttgcaccatatcataaaaatcgaaacagcaaagaatggcggaaacgtaaaagaagttatggaaataagacttagaagcaaacttaagagtgtgttgatagtgcagtatcttaaaattttgtataataggaattgaagttaaattagatgctaaaaatttgtaattaagaaggagtgattacatgaacaaaaatataaaatattctcaaaactttttaacgagtgaaaaagtactcaaccaaataataaaacaattgaatttaaaagaaaccgataccgtttacgaaattggaacaggtaaagggcatttaacgacgaaactggctaaaataagtaaacaggtaacgtctattgaattagacagtcatctattcaacttatcgtcagaaaaattaaaactgaatactcgtgtcactttaattcaccaagatattctacagtttcaattccctaacaaacagaggtataaaattgttgggagtattccttaccatttaagcacacaaattattaaaaaagtggtttttgaaagccatgcgtctgacatctatctgattgttgaagaaggattctacaagcgtaccttggatattcaccgaacactagggttgctcttgcacactcaagtctcgattcagcaattgcttaagctgccagcggaatgctttcatcctaaaccaaaagtaaacagtgtcttaataaaacttacccgccataccacagatgttccagataaatattggaagctatatacgtactttgtttcaaaatgggtcaatcgagaatatcgtcaactgtttactaaaaatcagtttcatcaagcaatgaaacacgccaaagtaaacaatttaagtaccgttacttatgagcaagtattgtctatttttaatagttatctattatttaacgggaggaaataattctatgagtcgcttttgtaaatttggaaagttacacgttactaaagggaatgtagataaattattaggtatactactgacagcttccaaggagctaaagaggtccctagcgcttagaatcgctttaggaaacacgatccagtccaataatcgtcgataaaaacttttgaaaaaggttggtgaaattacctacttttggaataatcacaaatcacaagtgattaatcacaaatcacaagtgattaatcacttgtttattaagatattaaaagctataatttaaataaagcgtgaattttattacacaaaaagaggggggagaaacttggaactagcatttagagaaagcttaaaaaagatgagaggtaccaaatcaaaagaaaaattctcccaagaattagaaatgagtagatcaaattattcacgaatagaatcaggaaaatcagatccaaccataaaaacactagaacaaattgcaaagttaactaactcaacgctagtagtggatttaatcccaaatgagccaacagaaccagaaccagaaacagaatcagaacaagtaacattggatttagaaatggaagaagaaaaaagcaatgacttcgtgtgaataatgcacgaaatcgttgcttatttttttttaaaagcggtatactagatataacgaaacaacgaactgaatagaaacgaaaaaagagccatgacacatttataaaatgtttgacgacattttataaatgcatagcccgataagattgccaaaccaacgcttatcagttagtcagatgaactcttccctcgtaagaagttatttaattaactttgtttgaagacggtatataaccgtactatcattatatagggaaatcagagagttttcaagtatctaagctactgaatttaagaattgttaagcaatcaatcggaaatcgtttgattgctttttttgtattcatttatagaaggtggagtttgtatgaatcatgatgaatgtaaaacttatataaaaaatagtttattggagataagaaaattagcaaatatctatacactagaaacgtttaagaaagagttagaaaagagaaatatctacttagaaacaaaatcagataagtatttttcttcggagggggaagattatatatataagttaatagaaaataacaaaataatttattcgattagtgga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BBa_K1830004_sequence 1 ccatcaacacagcctgtaacaacaccacctgcaacaacaaaaccacctgcaacaacaaaaccacctgcaacaacaataccgccgtcagatgatccgaatgcaataaagattaaggtggacacagtaaatgcaaaaccgggagacacagtaaatatacctgtaagattcagtggtataccatccaagggaatagcaaactgtgactttgtatacagctatgacccgaatgtacttgagataatagagataaaaccgggagaattgatagttgacccgaatcctgacaagagctttgatactgctgtatatcctgacagaaagataatagtattcctgtttgcagaagacagcggaacaggagcgtatgcaataactaaagacggagtatttgctacgatagtagcgaaagtaaaatccggagcacctaacggactcagtgtaatcaaatttgtagaagtaggcggatttgcgaacaatgaccttgtagaacagaggacacagttctttgacggtggagtaaatgttggagat BBa_K1830007_sequence 1 aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgctctagctaagcagaaggccatcctgacggatggcctttttgcgtttctacaaactc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z