This part is the main construct cloned by the iGEM Concordia 2015 team. It is the entire DNA sequence that allows for the Induction, expression, targeting and attachment of an extracellular scaffold onto the extracellular membrane of the lactic acid bacteria ''Lactococcus Lactis''. This scaffold contains two different dockerin domain that allow for the specific harboring of cohesin-fused proteins.
Promoter and ribosome binding site of the Lactococcal nisin inducible operon.
Nisin inducible promoter and ribosome binding site of the Lactococcal NisA gene
CCG to CCA mutation to remove AgeI cut site GAA to GAG mutation to remove EcoRI cut site
GeneBank CipA third type I cohesin domain
GCA to GCT mmutation to remove PstI cut site
GeneBank SdbA first type II cohesin domain, L. Lactis
GeneBank m6 protein, Streptococcus Pyogenes
Conversion of the iGEM parts registry to SBOL2.1