##gff-version 3
##sequence-region BBa_K1830017 1 7116
BBa_K1830017	.	sequence_feature	2538	7116	.	+	0	ID=BBa_K1830008;Name=BBa_K1830008
BBa_K1830017	.	sequence_feature	2538	7116	.	+	0	ID=annotation2434556;Name=Lactis Shuttle;Parent=BBa_K1830008
BBa_K1830017	.	polypeptide_domain	814	1344	.	+	0	ID=BBa_K1830004;Name=BBa_K1830004
BBa_K1830017	.	CDS	814	1344	.	+	0	ID=annotation2434513;Name=coh1C3;Parent=BBa_K1830004
BBa_K1830017	.	terminator	2374	2529	.	+	0	ID=BBa_K1830007;Name=BBa_K1830007
BBa_K1830017	.	stop_codon	2476	2520	.	+	0	ID=annotation2434523;Name=t2;Parent=BBa_K1830007
BBa_K1830017	.	stop_codon	2374	2471	.	+	0	ID=annotation2434522;Name=t1;Parent=BBa_K1830007
BBa_K1830017	.	polypeptide_domain	1353	1844	.	+	0	ID=BBa_K1830005;Name=BBa_K1830005
BBa_K1830017	.	CDS	1353	1844	.	+	0	ID=annotation2434516;Name=coh2S1;Parent=BBa_K1830005
BBa_K1830017	.	polypeptide_domain	302	805	.	+	0	ID=BBa_K1830003;Name=BBa_K1830003
BBa_K1830017	.	CDS	302	805	.	+	0	ID=annotation2434512;Name=CBD;Parent=BBa_K1830003
BBa_K1830017	.	polypeptide_domain	1853	2365	.	+	0	ID=BBa_K1830006;Name=BBa_K1830006
BBa_K1830017	.	CDS	1853	2365	.	+	0	ID=annotation2434520;Name=CWA;Parent=BBa_K1830006
BBa_K1830017	.	stop_codon	2270	2272	.	+	0	ID=annotation2434521;Name=TAA;Parent=BBa_K1830006
BBa_K1830017	.	conserved_region	2165	2179	.	+	0	ID=annotation2434557;Name=LPXTG;Parent=BBa_K1830006
BBa_K1830017	.	promoter	1	173	.	+	0	ID=BBa_K1830001;Name=BBa_K1830001
BBa_K1830017	.	operator	97	102	.	+	0	ID=annotation2434539;Name=-35;Parent=BBa_K1830001
BBa_K1830017	.	ribosome_entry_site	159	165	.	+	0	ID=annotation2434538;Name=RBS;Parent=BBa_K1830001
BBa_K1830017	.	promoter	1	173	.	+	0	ID=annotation2434537;Name=PnisA;Parent=BBa_K1830001
BBa_K1830017	.	operator	120	125	.	+	0	ID=annotation2434540;Name=-10;Parent=BBa_K1830001
BBa_K1830017	.	engineered_region	180	293	.	+	0	ID=BBa_K1830002;Name=BBa_K1830002
BBa_K1830017	.	start_codon	180	182	.	+	0	ID=annotation2434514;Name=ATG;Parent=BBa_K1830002
BBa_K1830017	.	sequence_feature	180	293	.	+	0	ID=annotation2434515;Name=Signal Peptide;Parent=BBa_K1830002
>BBa_K1830017
gaaacattaacaaatctaaaacagtcttaattctatcttgagaaagtattggtaataatattattgtcgataacgcgag
cataataaacggctctgattaaattctgaagtttgttagatacaatgatttcgttcgaaggaactacaaaataaattat
aaggaggcactcaaatactagatgaaaaaaaagattatctcagctattttaatgtctacagtgatactttctgctgctg
ccccgttgtcaggtgtttacgctgacacaaactcagatattgctaaacaagatgcgtactagagccggcaaatacacca
gtatcaggcaatttgaaggttgagttctacaacagcaatccttcagatactactaactcaatcaatcctcagttcaagg
ttactaataccggaagcagtgcaattgatttgtccaaactcacattgagatattattatacagtagacggacagaaaga
tcagaccttctggtgtgaccatgctgcaataatcggcagtaacggcagctacaacggaattacttcaaatgtaaaagga
acatttgtaaaaatgagttcctcaacaaataacgcagacacctaccttgaaataagctttacaggcggaactcttgaac
cgggtgcacatgttcagatacaaggtagatttgcaaagaatgactggagtaactatacacagtcaaatgactactcatt
caagtctgcttcacagtttgttgaatgggatcaggtaacagcatacttgaacggtgttcttgtatggggtaaagaaccc
ggtggcagtgtagtatactagagccatcaacacagcctgtaacaacaccacctgcaacaacaaaaccacctgcaacaac
aaaaccacctgcaacaacaataccgccgtcagatgatccgaatgcaataaagattaaggtggacacagtaaatgcaaaa
ccgggagacacagtaaatatacctgtaagattcagtggtataccatccaagggaatagcaaactgtgactttgtataca
gctatgacccgaatgtacttgagataatagagataaaaccgggagaattgatagttgacccgaatcctgacaagagctt
tgatactgctgtatatcctgacagaaagataatagtattcctgtttgcagaagacagcggaacaggagcgtatgcaata
actaaagacggagtatttgctacgatagtagcgaaagtaaaatccggagcacctaacggactcagtgtaatcaaatttg
tagaagtaggcggatttgcgaacaatgaccttgtagaacagaggacacagttctttgacggtggagtaaatgttggaga
ttactagaggataaagcctcgagcattgagcttaagtttgaccgcaataagggagaagttggagatatacttattggta
ccgtaaggataaacaatatcaagaatttcgcaggatttcaggtaaacattgtatatgatccaaaagtcttaatggctgt
tgaccctgaaacggggaaagaatttacttcttcaacatttccgccaggacgcactgtactgaaaaacaatgcttacggc
ccaatacagattgcggacaatgatccggaaaaagggatactgaacttcgcgcttgcatattcatatattgcgggataca
aagaaacaggagtagcggaggaaagcggcataattgcgaaaattggatttaaaatactccagaaaaagagcactgccgt
aaaattccaggatacattaagcatgcccggagctatttcgggaacacagctgtttgactgggacggagaagttattacc
ggatatgaggtaatacagccggataagtactagaggctttagaagaagcaaacagcaaattagctgctcttgaaaaact
taacaaagagcttgaagaaagcaagaaattaacagaaaaagaaaaagctgagctacaagcaaaacttgaagcagaagca
aaagcactcaaagaacaattagcgaaacaagctgaagaacttgcaaaactaagagctggaaaagcatcagactcacaaa
cccctgatgcaaaaccaggaaacaaagttgttccaggtaaaggtcaagcaccacaagcaggtacaaaacctaaccaaaa
caaagcaccaatgaaggaaactaagagacagttaccatcaacaggtgaaacagctaacccattcttcacagcggcagcc
cttactgttatggcaacagctggagtagcagcagttgtaaaacgcaaagaagaaaactaagctatcactttgtaatact
gagtgaacatcaagagagaaccagtcggttctctcttttatgtatagaagaatgagattaaggaggtcacaaactacta
gagaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tcctgagtaggacaaatccgccgctctagctaagcagaaggccatcctgacggatggcctttttgcgtttctacaaact
ctactagagagaatcgatacgattttgaagtggcaacagataaaaaaaagcagtttaaaattgttgctgaacttttaaa
acaagcaaatacaatcattgtcgcaacagatagcgacagagaaggcgaaaacattgcctggtcgatcattcataaagca
aatgccttttctaaagataaaacgtataaaagactatggatcaatagtttagaaaaagatgtgatccgtagcggttttc
aaaatttgcaaccaggaatgaattactatcccttttatcaagaagcgcaaaagaaaaacgaaatgatacaccaatcagt
gcaaaaaaagatataatgggagataagacggttcgtgttcgtgctgacttgcaccatatcataaaaatcgaaacagcaa
agaatggcggaaacgtaaaagaagttatggaaataagacttagaagcaaacttaagagtgtgttgatagtgcagtatct
taaaattttgtataataggaattgaagttaaattagatgctaaaaatttgtaattaagaaggagtgattacatgaacaa
aaatataaaatattctcaaaactttttaacgagtgaaaaagtactcaaccaaataataaaacaattgaatttaaaagaa
accgataccgtttacgaaattggaacaggtaaagggcatttaacgacgaaactggctaaaataagtaaacaggtaacgt
ctattgaattagacagtcatctattcaacttatcgtcagaaaaattaaaactgaatactcgtgtcactttaattcacca
agatattctacagtttcaattccctaacaaacagaggtataaaattgttgggagtattccttaccatttaagcacacaa
attattaaaaaagtggtttttgaaagccatgcgtctgacatctatctgattgttgaagaaggattctacaagcgtacct
tggatattcaccgaacactagggttgctcttgcacactcaagtctcgattcagcaattgcttaagctgccagcggaatg
ctttcatcctaaaccaaaagtaaacagtgtcttaataaaacttacccgccataccacagatgttccagataaatattgg
aagctatatacgtactttgtttcaaaatgggtcaatcgagaatatcgtcaactgtttactaaaaatcagtttcatcaag
caatgaaacacgccaaagtaaacaatttaagtaccgttacttatgagcaagtattgtctatttttaatagttatctatt
atttaacgggaggaaataattctatgagtcgcttttgtaaatttggaaagttacacgttactaaagggaatgtagataa
attattaggtatactactgacagcttccaaggagctaaagaggtccctagcgcttagaatcgctttaggaaacacgatc
cagtccaataatcgtcgataaaaacttttgaaaaaggttggtgaaattacctacttttggaataatcacaaatcacaag
tgattaatcacaaatcacaagtgattaatcacttgtttattaagatattaaaagctataatttaaataaagcgtgaatt
ttattacacaaaaagaggggggagaaacttggaactagcatttagagaaagcttaaaaaagatgagaggtaccaaatca
aaagaaaaattctcccaagaattagaaatgagtagatcaaattattcacgaatagaatcaggaaaatcagatccaacca
taaaaacactagaacaaattgcaaagttaactaactcaacgctagtagtggatttaatcccaaatgagccaacagaacc
agaaccagaaacagaatcagaacaagtaacattggatttagaaatggaagaagaaaaaagcaatgacttcgtgtgaata
atgcacgaaatcgttgcttatttttttttaaaagcggtatactagatataacgaaacaacgaactgaatagaaacgaaa
aaagagccatgacacatttataaaatgtttgacgacattttataaatgcatagcccgataagattgccaaaccaacgct
tatcagttagtcagatgaactcttccctcgtaagaagttatttaattaactttgtttgaagacggtatataaccgtact
atcattatatagggaaatcagagagttttcaagtatctaagctactgaatttaagaattgttaagcaatcaatcggaaa
tcgtttgattgctttttttgtattcatttatagaaggtggagtttgtatgaatcatgatgaatgtaaaacttatataaa
aaatagtttattggagataagaaaattagcaaatatctatacactagaaacgtttaagaaagagttagaaaagagaaat
atctacttagaaacaaaatcagataagtatttttcttcggagggggaagattatatatataagttaatagaaaataaca
aaataatttattcgattagtggaaaaaaattgacttataaaggaaaaaaatctttttcaaaacatgcaatattgaaaca
gttgaatgaaaaagcaaaccaagttaattaaacaacctattttataggatttataggaaaggagaacagctgaatgaat
atcccttttgttgtagaaactgtgcttcatgacggcttgttaaagtacaaatttaaaaatagtaaaattcgctcaatca
ctaccaagccaggtaaaagcaaaggggctatttttgcgtatcgctcaaaatcaagcatgattggcggtcgtggtgttgt
tctgacttccgaggaagcgattcaagaaaatcaagatacatttacacattggacacccaacgtttatcgttatggaacg
tatgcagacgaaaaccgttcatacacgaaaggacattctgaaaacaatttaagacaaatcaataccttctttattgatt
ttgatattcacacggcaaaagaaactatttcagcaagcgatattttaacaaccgctattgatttaggttttatgcctac
tatgattatcaaatctgataaaggttatcaagcatattttgttttagaaacgccagtctatgtgacttcaaaatcagaa
tttaaatctgtcaaagcagccaaaataatttcgcaaaatatccgagaatattttggaaagtctttgccagttgatctaa
cgtgtaatcattttggtattgctcgcataccaagaacggacaatgtagaattttttgatcctaattaccgttattcttt
caaagaatggcaagattggtctttcaaacaaacagataataagggctttactcgttcaagtctaacggttttaagcggt
acagaaggcaaaaaacaagtagatgaaccctggtttaatctcttattgcacgaaacgaaattttcaggagaaaagggtt
taatagggcgtaataacgtcatgtttaccctctctttagcctactttagttcaggctattcaatcgaaacgtgcgaata
taatatgtttgagtttaataatcgattagatcaacccttagaagaaaaagaagtaatcaaaattgttagaagtgcctat
tcagaaaactatcaaggggctaatagggaatacattaccattctttgcaaagcttgggtatcaagtgatttaaccagta
aagatttatttgtccgtcaagggtggtttaaattcaagaaaaaaagaagcgaacgtcaacgtgttcatttgtcagaatg
gaaagaagatttaatggcttatattagcgaaaaaagcgatgtatacaagccttatttagtgacgaccaaaaaagagatt
agagaagtgctaggcattcctgaacggacattagataaattgctgaaggtactgaaggcgaatcaggaaattttcttta
agattaaaccaggaagaaatggtggcattcaacttgctagtgttaaatcattgttgctatcgatcattaaagtaaaaaa
agaagaaaaagaaagctatataaaggcgctgacaaattcttttgacttagagcatacattcattcaagagactttaaac
aagctagcagaacgccctaaaacggacacacaactcgatttgtttagctatgatacaggctgaaaataaaacccgcact
atgccattacatttatatctatgatacgtgtttgttttttctttgctgtttagcgaatgattagcagaaatatacagag
taagattttaattaattattagggggagaaggagagagtagcccgaaaacttttagttggcttggactgaacgaagtga
gggaaaggctactaaaacgtcgaggggcagtgagagcgaagcgaacacttgattttttaattttctatcttttataggt
cattagagtatacttatttgtcctataaactatttagcagcataatagatttattgaataggtcatttaagttgagcat
attagaggaggaaaatcttggagaaatatttgaagaacccgattacatggattggattagttcttgtggttacgtggtt
tttaactaaaagtagtgaatttttgatttttggtgtgtgtgtcttgttgttagtatttgctagtcaaagtgattaaata
gacttc