LOCUS       BBa_K1831000             746 bp    DNA     linear       22-Nov-2024
DEFINITION  NpuDnaE intein for circularization of Type lll AFP+ inker
ACCESSION   BBa_K1831000
VERSION     BBa_K1831000.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     RBS             47..122
                     /label=BBa_K1362090
     CDS             123..185
                     /label=NpuDnaE(C)
     misc_feature    186..188
                     /label=BsaI scar
     CDS             189..197
                     /label=Extein(C) 
     CDS             198..200
                     /label=Linker
     CDS             201..401
                     /label=Type III AFP
     CDS             402..410
                     /label=Extein(N)
     misc_feature    411..413
                     /label=BsaI scar
     CDS             414..714
                     /label=NpuDnaE(N)
     stem_loop       715..746
                     /label=T7 terminator
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcaataa ttttgtttaa
       61 ctttaagaag gagatactag atgatcaaaa tagccacacg taaatattta ggcaaacaaa
      121 atgtctatga cattggagtt gagcgcgacc ataattttgc actcaaaaat ggcttcatag
      181 cttccaactg ctgggaaggc gcggcgaacc aggcgagcgt ggtggcgaac cagctgattc
      241 cgattaacac cgcgctgacc ctggtgatga tgcgcagcga agtggtgacc ccggtgggca
      301 ttccggcgga agatattccg cgcctggtga gcatgcaggt gaaccgcgcg gtgccgctgg
      361 gcaccaccct gatgccggat atggtgaaag gctatgcggc gcgtggtaaa tgcttaagct
      421 atgaaacgga aatattgaca gtagaatatg gattattacc gattggtaaa attgtagaaa
      481 agcgcatcga atgtactgtt tatagcgttg ataataatgg aaatatttat acacaacctg
      541 tagcacaatg gcacgatcgc ggagaacaag aggtgtttga gtattgtttg gaagatggtt
      601 cattgattcg ggcaacaaaa gaccataagt ttatgactgt tgatggtcaa atgttgccaa
      661 ttgatgaaat atttgaacgt gaattggatt tgatgcgggt tgataatttg ccgaattaat
      721 aaatcggtga aatgcacgac tgatag
//