LOCUS       BBa_K1831001             363 bp    DNA     linear       16-May-2024
DEFINITION  TypeIII and AFP E-coil fusion + His tag
ACCESSION   BBa_K1831001
VERSION     BBa_K1831001.1  
FEATURES             Location/Qualifiers
     promoter        1..61
                     /label=T7 rbs
     misc_feature    62..67
                     /label=NdeI site
     CDS             68..267
                     /label=TypeIII AFP
     CDS             268..330
                     /label=E-coil
     misc_feature    331..336
                     /label=BamHI site
     misc_feature    337..351
                     /label=His-tag
     misc_feature    358..363
                     /label=XhoI site
     misc_feature    352..357
                     /label=stop x2
ORIGIN
        1 tgtaagttta tacataggcg agtactctgt tatggtccag agaaagaggg gacaaaccag
       61 tcatatggcg gcgaaccagg cgagcgtggt ggcgaaccag ctgattccga ttaacaccgc
      121 gctgaccctg gtgatgatgc gcagcgaagt ggtgaccccg gtgggcattc cggcggaaga
      181 tattccgcgc ctggtgagca tgaggtgaac cgcgcggtgc cgctgggcac caccctgatg
      241 ccggatatgg tgaaaggcta tgcggcggaa attgcggcgc tggaaaaaga gatcgctgcg
      301 ctggaaaagg aaattgcggc cctggagaag ggatcccatc atcatcatca ttaataactc
      361 gag
//