##gff-version 3
##sequence-region BBa_K1831001 1 363
BBa_K1831001	.	stop_codon	352	357	.	+	0	ID=annotation2450033;Name=stop x2
BBa_K1831001	.	tag	337	351	.	+	0	ID=annotation2449966;Name=His-tag
BBa_K1831001	.	CDS	68	267	.	+	0	ID=annotation2449963;Name=TypeIII AFP
BBa_K1831001	.	sequence_feature	62	67	.	+	0	ID=annotation2449962;Name=NdeI site
BBa_K1831001	.	sequence_feature	358	363	.	+	0	ID=annotation2449967;Name=XhoI site
BBa_K1831001	.	sequence_feature	331	336	.	+	0	ID=annotation2449965;Name=BamHI site
BBa_K1831001	.	CDS	268	330	.	+	0	ID=annotation2449964;Name=E-coil
BBa_K1831001	.	promoter	1	61	.	+	0	ID=annotation2449961;Name=T7 rbs
>BBa_K1831001
tgtaagtttatacataggcgagtactctgttatggtccagagaaagaggggacaaaccagtcatatggcggcgaaccag
gcgagcgtggtggcgaaccagctgattccgattaacaccgcgctgaccctggtgatgatgcgcagcgaagtggtgaccc
cggtgggcattccggcggaagatattccgcgcctggtgagcatgaggtgaaccgcgcggtgccgctgggcaccaccctg
atgccggatatggtgaaaggctatgcggcggaaattgcggcgctggaaaaagagatcgctgcgctggaaaaggaaattg
cggccctggagaagggatcccatcatcatcatcattaataactcgag