BBa_K1833008 1 BBa_K1833008 pT7-MMP9 sensitive repressor 2015-09-16T11:00:00Z 2015-09-18T07:43:05Z The two protein domains comprising the fusion protein was used as in "A two-hybrid system based on chimeric operator recognition for studying protein homo/heterodimerization in Escherichia coli" (G. Di Lallo, L. Castagnoli, P. Ghelardini and L. Paolozzi, Microbiology (2001), 147, 1651???1656). The linker sequence was used from "Fluorescence Detection of MMP-9. I. MMP-9 Selectively Cleaves Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys Peptide" (Rafal Fudala et al, Curr Pharm Biotechnol. 2011 May ; 12(5): 834???838.) This part produces a synthetic repressor in response to T7 RNA Polymerase. This synthetic repressor consists of a fusion of two DNA binding domains connected with a linker that is sensitive to matrix metalloprotease 9, and it represses the promoter described by parts BBa_K1833001 and BBa_K1833003. For more information on this part, visit 2015.igem.org/Team:Pitt/Protease/Project. false false _2259_ 27388 27388 9 false The part was synthesized <I>de novo</I>. For simple cloning, there are no smaller parts used to make this part. false Konstantin Borisov BBa_K1833008_sequence 1 taatacgactcactatagggaaagaggagaaaatgtcgatcagctcgcgggtgaagagcaaacggatccagctgggtctgaaccaggcggaacttgcgcagaaagtgggcacgacacagcagagcattgaacagctggagaatggcaaaaccaaacgcccacgctttctgccagagcttgcgagtgccctgggggtctccgtcgattggctcctgaacggcacttcagacagcaacgtgcgctttgtgggtcatgttgaaccgaaaggtaagtatccgctgatctcgatggtccgggccggctcttggtgtgaggcatgtgagccatatgatatcaaaggagggggttcgggcggtggaggctccggcggggggggaccgcgttcgttgtccggcggcggagggtcaggcggcggtggcagcggtggcggaatgtcaattagctctcgtgtgaaatctaaacgcattcagttgggcctgaatcaagcggaacttgcacagaaagttggtacttcaaatgtctccatctcacagctggagcgcggcaaaaccaaacgtccccgcttcctgcccgaactggcatcagccctgggcgtgagtgtcgattggctgttaaacggtacaagcgattccaacgtgcgttttgtgggccatgtggagccgaagggaaagtatccgctgatctccatggtccgcgccggctcgtggtgctaatgaaaccccttggggcctctaaacgggtcttgaggggttttttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z