LOCUS BBa_K1834000 2250 bp DNA linear 27-Dec-2024 DEFINITION Xylene Sensor! ACCESSION BBa_K1834000 VERSION BBa_K1834000.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 704..709 /label=PstI site CDS 62..1762 /label=XylR CDS 62..1762 /label=BBa_I723017 stem_loop 1782..1825 /label=stem_loop misc_feature 1771..1850 /label=BBa_B0010 terminator 1771..1850 /label=BBa_B0010 stem_loop 1866..1885 /label=T7 TE misc_feature 1892..1892 /label=stop polyA_site 1886..1899 /label=polya misc_feature 1859..1899 /label=BBa_B0012 terminator 1859..1899 /label=BBa_B0012 terminator 1771..1899 /label=BBa_B0015 promoter 1908..2227 /label=Pu promoter 1908..2227 /label=BBa_I723020 RBS 2236..2250 /label=RBS-1\Strong RBS 2243..2247 /label=RBS misc_feature 2236..2250 /label=BBa_B0030 RBS 2236..2250 /label=BBa_B0030 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatgtcgctt acatacaaac ccaagatgca gcatgaggat atgcaagacc ttagcagcca 121 gatccgtttc gttgccgccg aaggcaagat ctggttggga gagcagcgca tgctcgtaat 181 gcagctatct acgctggcca gcttccgtcg cgaaattatc agcttgatcg gcgtcgagcg 241 ggccaagggt ttcttcctgc ggttgggcta tcagtccggc ctgatggatg ccgagctggc 301 acgcaagctg cggccggcca tgcgcgagga ggaggtgttc ctggctgggc ctcaattgta 361 tgcgctcaag gggatggtca aagtacgctt gctgacaatg gatatcgcca tccgggacgg 421 acgtttcaac gtggaggccg agtggattga ttcctttgaa gtggatatct gccgaactga 481 gctgggcctg atgaatgagc ccgtctgctg gacggtgcta ggctatgcta gcggctatgg 541 ttcggcattc atgggccgca gaatcatttt ccaggaaact agctgtcgcg ggtgcggtga 601 cgataaatgc cttatcgtcg gcaagaccgc agaagagtgg ggcgatgtca gcagtttcga 661 agcctacttc aaaagcgacc cgatcgtaga cgagcgctac gagctgcaga cccaggttgc 721 caacctgcgc aaccgcctga agcagtacga tgggcagtat tacggcattg gccattcgcc 781 agcctacaag cgcatctgtg agaccatcga caaggctgca cgcggcaggg tttcggtcct 841 gctactgggt gagactgggg tgggcaagga ggtaatcgcg cgcagcgtgc atttgcgcag 901 tgagcgcgca gagcaaccct tcgtcgcggt gaactgtgcg gcaattccgc cggatctgat 961 cgagtcggaa ctgtttggtg tcgataaggg cgcctatacg ggcgcggtca atgcacgcgc 1021 tggacgtttt gaacgggcca acggcggcac catctttctt gatgaggtga tcgaattgac 1081 gccgagggcc caggccaccc tgctacgggt attgcaggaa ggagagctag agcgggtcgg 1141 cggcgaccgc acgcgaaagg tcgacgtgag gttaatcacc gcaacaaacg agaacctgga 1201 agaggcggtc aagatggggc gctttcgcgc agacctgttc tttcggctga atgtttttcc 1261 cgtgcatatc ccgccgttgc gcgagcgcgt ggaagatatc ccgctgctgg tcgagcattt 1321 tcttagaagg caccataagg aatacggtaa gaagactctt ggcctgtctg atcgagcgat 1381 ggaggcctgc ctccactacc aatggccagg caatatccgc gagctggaga acgcccttga 1441 gcgcggggtg attcttaccg agagcaacga aagcatcaat gtcgagtcgc tgttcccggg 1501 gttggcgacg gctaccgaag gcgacaggct atcgagcgag ggccggttgg aggaggagtc 1561 cggtgacagt tggtttaggc aaattatcga ccagggcgtc agcctcgaag atctcgaagc 1621 gggtttaatg cgcacggcca tggaccgttg tgggcagaat atctcacagg cggcgcggtt 1681 gctgggattg acccgcccgg caatggccta tcgacttaag aagcttgacc ccagcttatc 1741 tgtgaaagca atgggccgat agtactagag ccaggcatca aataaaacga aaggctcagt 1801 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 1861 acactggctc accttcgggt gggcctttct gcgtttatat actagagccc gggaaagcgc 1921 gatgaacctt ttttatcgct gccttgatca aatcgacagg tggttatgcg cgattgatga 1981 tttgctcaaa tacagccagc gtgctgtaga ttttctctca taccccccct ttctttttta 2041 caaagaaaat caataattta gatgaaataa ggggatcggt ataagcaatg gcatggcggt 2101 tgctagctat acgagactta aaataaaaat agtggtgacc cttcaatgtt gtattttctc 2161 aactctgttc agattggttg ctttcgccat gtatatcctc aaagcgggcc agccgtagcc 2221 gttacgctac tagagattaa agaggagaaa //