BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_K1834845
1
BBa_K1834845
Xylene Sensor!
2015-09-07T11:00:00Z
2015-09-08T04:26:38Z
E. Coli
Produces xylR constantly and when in the presence of xylene, the Pu promoter is activated.
false
false
_2260_
15367
15367
9
false
N/A
false
Gerry Bourassa
component2447380
1
BBa_B0034
component2447388
1
BBa_B0015
component2447381
1
BBa_K1834844
component2447378
1
BBa_J23100
component2447392
1
BBa_B0030
component2447390
1
BBa_I723020
annotation2447388
1
BBa_B0015
range2447388
1
1865
1993
annotation2447390
1
BBa_I723020
range2447390
1
2002
2321
annotation2447381
1
BBa_K1834844
range2447381
1
62
1856
annotation2447380
1
BBa_B0034
range2447380
1
44
55
annotation2447378
1
BBa_J23100
range2447378
1
1
35
annotation2447392
1
BBa_B0030
range2447392
1
2330
2344
BBa_K1834844
1
BBa_K1834844
xylR gene
2015-09-07T11:00:00Z
2015-09-08T04:22:09Z
E. Coli
xylR regulatory unit.
false
false
_2260_
15367
15367
9
false
The same as a previously generated part but we removed the illegal Pst1 site.
false
Gerry Bourassa
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_I723020
1
Pu
Pu
2007-10-24T11:00:00Z
2015-08-31T04:07:54Z
[Christine/Maia]
This is the promoter activated by the transcriptional regulator XylR (BBa_I723136) in response to detection of environmental xylene.
false
false
_126_
0
2140
9
It's complicated
false
[Christine/Maia]
true
Scott Ramsay
annotation1955192
1
Pu
range1955192
1
1
320
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1834844_sequence
1
atgagcttgatctataagccgaaaatgcagcacgaagatatgcaggatctgatctcgcaaattcgttttgtagcggcggaaggtaagatctggcttggcgaacagcgcatgctgctcatgcagctgtccacactggcatcgtttcgccgtgaaattattagcttaattggtattgaacgtgccaagggatttttcctgcgtctgggttatcagagcggattgatggacgcagagctggctcgcaaactgcgtccggcgatgcgtgaggaagaggttttcttagcgggccctcaattgtacgcgttaaaaggcatggttaaagtacgtctgctgacaatggacatcgcgattcgcgatggccgcttcaacgtggaggccgaatggattgattcgtttgaggtggatatttgccgcactgagctgggtcttatgaacgagcctgtctgttggaccgttctgggttacgcatcagggtatgggagtgcgtttatgggccgccgcatcatctttcaagaaacttcttgtcgtggttgtggcgatgataaatgcctgatcgtgggcaaaaccgcggaggagtggggggatgtgagttcctttgaagcctattttaaatccgaccccattgttgacgagcgctatgaactgcaaacccaggtggctaatctgcgcaatcgtttaaaacagtacgatggccaatattacggaattgggcactcaccggcgtacaaacgcatttgtgaaaccattgataaagccgctcgtggtaaagttagcgtgctgttgctgggtgagaccggagtaggaaaagaagtgattgcgcgtagtgtgcatttacgtagcgaacgtgcggaacagccgtttgttgcggtcaactgcgcggccattcctccggatctgattgaaagtgaactgtttggtgtggataaaggggcctataccggtgcggtcaatgctcgtgcgggccgctttgagcgcgccaacggtggtaccattttcctggatgaagttattgaactcaccccgcgcgcccaagccacccttctgcgtgttctccaggaaggagagctggaacgtgttggtggtgatcgcacccgtaaggttgatgtgcgtctgatcacggcaaccaacgagaatctggaagaagcggttaaaatggggcgctttcgtgcagatctgtttttccgcttaaacgtcttcccggtccaaatcccgccactgcgtgaacgcgtggaagacattcccttactggtggaacattttttacgccgtcatcacaaagagtatggtaagaaaacgttaggtttatcagatcgcgcaatggaagcgtgcctgcactatcagtggcctgggaacatccgcgagcttgaaaatgcgctggaacgtggggtgatcctgaccgaatcaaatgaaagcattaacgttgaatccctctttcccggcctggcaatggcaactgaaggggaccgcctttcctcggaagggcgtctggaagaggaatctggtgattcatggtttcgccagattattgatcagggtgtctcattagaagatctggaggcaggtctgatgcgtacagcgatggatcgctgcggccaaaacatttctcaggcggcgcgccttctcggccttacgcgcccggcgatggcataccgcctgaaaaaacttgatccgtcgctgagcgtgaaagccactggccgctgataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1834845_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgagcttgatctataagccgaaaatgcagcacgaagatatgcaggatctgatctcgcaaattcgttttgtagcggcggaaggtaagatctggcttggcgaacagcgcatgctgctcatgcagctgtccacactggcatcgtttcgccgtgaaattattagcttaattggtattgaacgtgccaagggatttttcctgcgtctgggttatcagagcggattgatggacgcagagctggctcgcaaactgcgtccggcgatgcgtgaggaagaggttttcttagcgggccctcaattgtacgcgttaaaaggcatggttaaagtacgtctgctgacaatggacatcgcgattcgcgatggccgcttcaacgtggaggccgaatggattgattcgtttgaggtggatatttgccgcactgagctgggtcttatgaacgagcctgtctgttggaccgttctgggttacgcatcagggtatgggagtgcgtttatgggccgccgcatcatctttcaagaaacttcttgtcgtggttgtggcgatgataaatgcctgatcgtgggcaaaaccgcggaggagtggggggatgtgagttcctttgaagcctattttaaatccgaccccattgttgacgagcgctatgaactgcaaacccaggtggctaatctgcgcaatcgtttaaaacagtacgatggccaatattacggaattgggcactcaccggcgtacaaacgcatttgtgaaaccattgataaagccgctcgtggtaaagttagcgtgctgttgctgggtgagaccggagtaggaaaagaagtgattgcgcgtagtgtgcatttacgtagcgaacgtgcggaacagccgtttgttgcggtcaactgcgcggccattcctccggatctgattgaaagtgaactgtttggtgtggataaaggggcctataccggtgcggtcaatgctcgtgcgggccgctttgagcgcgccaacggtggtaccattttcctggatgaagttattgaactcaccccgcgcgcccaagccacccttctgcgtgttctccaggaaggagagctggaacgtgttggtggtgatcgcacccgtaaggttgatgtgcgtctgatcacggcaaccaacgagaatctggaagaagcggttaaaatggggcgctttcgtgcagatctgtttttccgcttaaacgtcttcccggtccaaatcccgccactgcgtgaacgcgtggaagacattcccttactggtggaacattttttacgccgtcatcacaaagagtatggtaagaaaacgttaggtttatcagatcgcgcaatggaagcgtgcctgcactatcagtggcctgggaacatccgcgagcttgaaaatgcgctggaacgtggggtgatcctgaccgaatcaaatgaaagcattaacgttgaatccctctttcccggcctggcaatggcaactgaaggggaccgcctttcctcggaagggcgtctggaagaggaatctggtgattcatggtttcgccagattattgatcagggtgtctcattagaagatctggaggcaggtctgatgcgtacagcgatggatcgctgcggccaaaacatttctcaggcggcgcgccttctcggccttacgcgcccggcgatggcataccgcctgaaaaaacttgatccgtcgctgagcgtgaaagccactggccgctgataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgctactagagattaaagaggagaaa
BBa_I723020_sequence
1
cccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z