LOCUS       BBa_K1834846            3019 bp    DNA     linear       22-Nov-2024
DEFINITION  Xylene Sensor! With AmilCP!
ACCESSION   BBa_K1834846
VERSION     BBa_K1834846.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1856
                     /label=BBa_K1834844
     stem_loop       1876..1919
                     /label=stem_loop
     misc_feature    1865..1944
                     /label=BBa_B0010
     terminator      1865..1944
                     /label=BBa_B0010
     stem_loop       1960..1979
                     /label=T7 TE
     misc_feature    1986..1986
                     /label=stop
     polyA_site      1980..1993
                     /label=polya
     misc_feature    1953..1993
                     /label=BBa_B0012
     terminator      1953..1993
                     /label=BBa_B0012
     terminator      1865..1993
                     /label=BBa_B0015
     promoter        2002..2321
                     /label=Pu
     promoter        2002..2321
                     /label=BBa_I723020
     RBS             2330..2344
                     /label=RBS-1\Strong
     RBS             2337..2341
                     /label=RBS
     misc_feature    2330..2344
                     /label=BBa_B0030
     RBS             2330..2344
                     /label=BBa_B0030
     misc_feature    1..2344
                     /label=BBa_K1834845
     CDS             2351..3016
                     /label=amilCP
     CDS             2351..3019
                     /label=BBa_K592009
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgagcttg atctataagc cgaaaatgca gcacgaagat atgcaggatc tgatctcgca
      121 aattcgtttt gtagcggcgg aaggtaagat ctggcttggc gaacagcgca tgctgctcat
      181 gcagctgtcc acactggcat cgtttcgccg tgaaattatt agcttaattg gtattgaacg
      241 tgccaaggga tttttcctgc gtctgggtta tcagagcgga ttgatggacg cagagctggc
      301 tcgcaaactg cgtccggcga tgcgtgagga agaggttttc ttagcgggcc ctcaattgta
      361 cgcgttaaaa ggcatggtta aagtacgtct gctgacaatg gacatcgcga ttcgcgatgg
      421 ccgcttcaac gtggaggccg aatggattga ttcgtttgag gtggatattt gccgcactga
      481 gctgggtctt atgaacgagc ctgtctgttg gaccgttctg ggttacgcat cagggtatgg
      541 gagtgcgttt atgggccgcc gcatcatctt tcaagaaact tcttgtcgtg gttgtggcga
      601 tgataaatgc ctgatcgtgg gcaaaaccgc ggaggagtgg ggggatgtga gttcctttga
      661 agcctatttt aaatccgacc ccattgttga cgagcgctat gaactgcaaa cccaggtggc
      721 taatctgcgc aatcgtttaa aacagtacga tggccaatat tacggaattg ggcactcacc
      781 ggcgtacaaa cgcatttgtg aaaccattga taaagccgct cgtggtaaag ttagcgtgct
      841 gttgctgggt gagaccggag taggaaaaga agtgattgcg cgtagtgtgc atttacgtag
      901 cgaacgtgcg gaacagccgt ttgttgcggt caactgcgcg gccattcctc cggatctgat
      961 tgaaagtgaa ctgtttggtg tggataaagg ggcctatacc ggtgcggtca atgctcgtgc
     1021 gggccgcttt gagcgcgcca acggtggtac cattttcctg gatgaagtta ttgaactcac
     1081 cccgcgcgcc caagccaccc ttctgcgtgt tctccaggaa ggagagctgg aacgtgttgg
     1141 tggtgatcgc acccgtaagg ttgatgtgcg tctgatcacg gcaaccaacg agaatctgga
     1201 agaagcggtt aaaatggggc gctttcgtgc agatctgttt ttccgcttaa acgtcttccc
     1261 ggtccaaatc ccgccactgc gtgaacgcgt ggaagacatt cccttactgg tggaacattt
     1321 tttacgccgt catcacaaag agtatggtaa gaaaacgtta ggtttatcag atcgcgcaat
     1381 ggaagcgtgc ctgcactatc agtggcctgg gaacatccgc gagcttgaaa atgcgctgga
     1441 acgtggggtg atcctgaccg aatcaaatga aagcattaac gttgaatccc tctttcccgg
     1501 cctggcaatg gcaactgaag gggaccgcct ttcctcggaa gggcgtctgg aagaggaatc
     1561 tggtgattca tggtttcgcc agattattga tcagggtgtc tcattagaag atctggaggc
     1621 aggtctgatg cgtacagcga tggatcgctg cggccaaaac atttctcagg cggcgcgcct
     1681 tctcggcctt acgcgcccgg cgatggcata ccgcctgaaa aaacttgatc cgtcgctgag
     1741 cgtgaaagcc actggccgct gataataata ctagagccag gcatcaaata aaacgaaagg
     1801 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1861 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1921 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1981 ttctgcgttt atatactaga gcccgggaaa gcgcgatgaa ccttttttat cgctgccttg
     2041 atcaaatcga caggtggtta tgcgcgattg atgatttgct caaatacagc cagcgtgctg
     2101 tagattttct ctcatacccc ccctttcttt tttacaaaga aaatcaataa tttagatgaa
     2161 ataaggggat cggtataagc aatggcatgg cggttgctag ctatacgaga cttaaaataa
     2221 aaatagtggt gacccttcaa tgttgtattt tctcaactct gttcagattg gttgctttcg
     2281 ccatgtatat cctcaaagcg ggccagccgt agccgttacg ctactagaga ttaaagagga
     2341 gaaatactag atgagtgtga tcgctaaaca aatgacctac aaggtttata tgtcaggcac
     2401 ggtcaatgga cactactttg aggtcgaagg cgatggaaaa ggtaagccct acgaggggga
     2461 gcagacggta aagctcactg tcaccaaggg cggacctctg ccatttgctt gggatatttt
     2521 atcaccacag tgtcagtacg gaagcatacc attcaccaag taccctgaag acatccctga
     2581 ctatgtaaag cagtcattcc cggagggcta tacatgggag aggatcatga actttgaaga
     2641 tggtgcagtg tgtactgtca gcaatgattc cagcatccaa ggcaactgtt tcatctacca
     2701 tgtcaagttc tctggtttga actttcctcc caatggacct gtcatgcaga agaagacaca
     2761 gggctgggaa cccaacactg agcgtctctt tgcacgagat ggaatgctgc taggaaacaa
     2821 ctttatggct ctgaagttag aaggaggcgg tcactatttg tgtgaattta aaactactta
     2881 caaggcaaag aagcctgtga agatgccagg gtatcactat gttgaccgca aactggatgt
     2941 aaccaatcac aacaaggatt acacttcggt tgagcagtgt gaaatttcca ttgcacgcaa
     3001 acctgtggtc gcctaataa
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