BBa_K1834845 1 BBa_K1834845 Xylene Sensor! 2015-09-07T11:00:00Z 2015-09-08T04:26:38Z E. Coli Produces xylR constantly and when in the presence of xylene, the Pu promoter is activated. false false _2260_ 15367 15367 9 false N/A false Gerry Bourassa component2447388 1 BBa_B0015 component2447390 1 BBa_I723020 component2447378 1 BBa_J23100 component2447380 1 BBa_B0034 component2447381 1 BBa_K1834844 component2447392 1 BBa_B0030 annotation2447392 1 BBa_B0030 range2447392 1 2330 2344 annotation2447390 1 BBa_I723020 range2447390 1 2002 2321 annotation2447380 1 BBa_B0034 range2447380 1 44 55 annotation2447388 1 BBa_B0015 range2447388 1 1865 1993 annotation2447381 1 BBa_K1834844 range2447381 1 62 1856 annotation2447378 1 BBa_J23100 range2447378 1 1 35 BBa_K592009 1 amilCP amilCP, blue chromoprotein 2011-09-17T11:00:00Z 2015-05-08T01:12:48Z Acropora millepora Released HQ 2013 This chromoprotein, amilCP, naturally exhibits very strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. This DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of one illegal internal restriction site (EcoRI). false false _763_ 0 7929 9 In stock true Illegal internal restriction site had to be removed (EcoRI). false Lei Sun annotation2131628 1 amilCP range2131628 1 1 666 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1834847 1 BBa_K1834847 Xylene Sensor! With AmilCP! 2015-09-07T11:00:00Z 2015-09-08T04:32:14Z E. Coli :) Constantly produces xylR and in the presence of xylene. The Pu promoter is activated and AmilCP is produced. false false _2260_ 15367 15367 9 false N/A false Gerry Bourassa component2447431 1 BBa_K1834845 component2447433 1 BBa_K592009 annotation2447433 1 BBa_K592009 range2447433 1 2351 3019 annotation2447431 1 BBa_K1834845 range2447431 1 1 2344 BBa_K1834844 1 BBa_K1834844 xylR gene 2015-09-07T11:00:00Z 2015-09-08T04:22:09Z E. Coli xylR regulatory unit. false false _2260_ 15367 15367 9 false The same as a previously generated part but we removed the illegal Pst1 site. false Gerry Bourassa BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_I723020 1 Pu Pu 2007-10-24T11:00:00Z 2015-08-31T04:07:54Z [Christine/Maia] This is the promoter activated by the transcriptional regulator XylR (BBa_I723136) in response to detection of environmental xylene. false false _126_ 0 2140 9 It's complicated false [Christine/Maia] true Scott Ramsay annotation1955192 1 Pu range1955192 1 1 320 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1834844_sequence 1 atgagcttgatctataagccgaaaatgcagcacgaagatatgcaggatctgatctcgcaaattcgttttgtagcggcggaaggtaagatctggcttggcgaacagcgcatgctgctcatgcagctgtccacactggcatcgtttcgccgtgaaattattagcttaattggtattgaacgtgccaagggatttttcctgcgtctgggttatcagagcggattgatggacgcagagctggctcgcaaactgcgtccggcgatgcgtgaggaagaggttttcttagcgggccctcaattgtacgcgttaaaaggcatggttaaagtacgtctgctgacaatggacatcgcgattcgcgatggccgcttcaacgtggaggccgaatggattgattcgtttgaggtggatatttgccgcactgagctgggtcttatgaacgagcctgtctgttggaccgttctgggttacgcatcagggtatgggagtgcgtttatgggccgccgcatcatctttcaagaaacttcttgtcgtggttgtggcgatgataaatgcctgatcgtgggcaaaaccgcggaggagtggggggatgtgagttcctttgaagcctattttaaatccgaccccattgttgacgagcgctatgaactgcaaacccaggtggctaatctgcgcaatcgtttaaaacagtacgatggccaatattacggaattgggcactcaccggcgtacaaacgcatttgtgaaaccattgataaagccgctcgtggtaaagttagcgtgctgttgctgggtgagaccggagtaggaaaagaagtgattgcgcgtagtgtgcatttacgtagcgaacgtgcggaacagccgtttgttgcggtcaactgcgcggccattcctccggatctgattgaaagtgaactgtttggtgtggataaaggggcctataccggtgcggtcaatgctcgtgcgggccgctttgagcgcgccaacggtggtaccattttcctggatgaagttattgaactcaccccgcgcgcccaagccacccttctgcgtgttctccaggaaggagagctggaacgtgttggtggtgatcgcacccgtaaggttgatgtgcgtctgatcacggcaaccaacgagaatctggaagaagcggttaaaatggggcgctttcgtgcagatctgtttttccgcttaaacgtcttcccggtccaaatcccgccactgcgtgaacgcgtggaagacattcccttactggtggaacattttttacgccgtcatcacaaagagtatggtaagaaaacgttaggtttatcagatcgcgcaatggaagcgtgcctgcactatcagtggcctgggaacatccgcgagcttgaaaatgcgctggaacgtggggtgatcctgaccgaatcaaatgaaagcattaacgttgaatccctctttcccggcctggcaatggcaactgaaggggaccgcctttcctcggaagggcgtctggaagaggaatctggtgattcatggtttcgccagattattgatcagggtgtctcattagaagatctggaggcaggtctgatgcgtacagcgatggatcgctgcggccaaaacatttctcaggcggcgcgccttctcggccttacgcgcccggcgatggcataccgcctgaaaaaacttgatccgtcgctgagcgtgaaagccactggccgctgataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_B0030_sequence 1 attaaagaggagaaa BBa_K592009_sequence 1 atgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataa BBa_K1834845_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgagcttgatctataagccgaaaatgcagcacgaagatatgcaggatctgatctcgcaaattcgttttgtagcggcggaaggtaagatctggcttggcgaacagcgcatgctgctcatgcagctgtccacactggcatcgtttcgccgtgaaattattagcttaattggtattgaacgtgccaagggatttttcctgcgtctgggttatcagagcggattgatggacgcagagctggctcgcaaactgcgtccggcgatgcgtgaggaagaggttttcttagcgggccctcaattgtacgcgttaaaaggcatggttaaagtacgtctgctgacaatggacatcgcgattcgcgatggccgcttcaacgtggaggccgaatggattgattcgtttgaggtggatatttgccgcactgagctgggtcttatgaacgagcctgtctgttggaccgttctgggttacgcatcagggtatgggagtgcgtttatgggccgccgcatcatctttcaagaaacttcttgtcgtggttgtggcgatgataaatgcctgatcgtgggcaaaaccgcggaggagtggggggatgtgagttcctttgaagcctattttaaatccgaccccattgttgacgagcgctatgaactgcaaacccaggtggctaatctgcgcaatcgtttaaaacagtacgatggccaatattacggaattgggcactcaccggcgtacaaacgcatttgtgaaaccattgataaagccgctcgtggtaaagttagcgtgctgttgctgggtgagaccggagtaggaaaagaagtgattgcgcgtagtgtgcatttacgtagcgaacgtgcggaacagccgtttgttgcggtcaactgcgcggccattcctccggatctgattgaaagtgaactgtttggtgtggataaaggggcctataccggtgcggtcaatgctcgtgcgggccgctttgagcgcgccaacggtggtaccattttcctggatgaagttattgaactcaccccgcgcgcccaagccacccttctgcgtgttctccaggaaggagagctggaacgtgttggtggtgatcgcacccgtaaggttgatgtgcgtctgatcacggcaaccaacgagaatctggaagaagcggttaaaatggggcgctttcgtgcagatctgtttttccgcttaaacgtcttcccggtccaaatcccgccactgcgtgaacgcgtggaagacattcccttactggtggaacattttttacgccgtcatcacaaagagtatggtaagaaaacgttaggtttatcagatcgcgcaatggaagcgtgcctgcactatcagtggcctgggaacatccgcgagcttgaaaatgcgctggaacgtggggtgatcctgaccgaatcaaatgaaagcattaacgttgaatccctctttcccggcctggcaatggcaactgaaggggaccgcctttcctcggaagggcgtctggaagaggaatctggtgattcatggtttcgccagattattgatcagggtgtctcattagaagatctggaggcaggtctgatgcgtacagcgatggatcgctgcggccaaaacatttctcaggcggcgcgccttctcggccttacgcgcccggcgatggcataccgcctgaaaaaacttgatccgtcgctgagcgtgaaagccactggccgctgataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgctactagagattaaagaggagaaa BBa_I723020_sequence 1 cccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1834847_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgagcttgatctataagccgaaaatgcagcacgaagatatgcaggatctgatctcgcaaattcgttttgtagcggcggaaggtaagatctggcttggcgaacagcgcatgctgctcatgcagctgtccacactggcatcgtttcgccgtgaaattattagcttaattggtattgaacgtgccaagggatttttcctgcgtctgggttatcagagcggattgatggacgcagagctggctcgcaaactgcgtccggcgatgcgtgaggaagaggttttcttagcgggccctcaattgtacgcgttaaaaggcatggttaaagtacgtctgctgacaatggacatcgcgattcgcgatggccgcttcaacgtggaggccgaatggattgattcgtttgaggtggatatttgccgcactgagctgggtcttatgaacgagcctgtctgttggaccgttctgggttacgcatcagggtatgggagtgcgtttatgggccgccgcatcatctttcaagaaacttcttgtcgtggttgtggcgatgataaatgcctgatcgtgggcaaaaccgcggaggagtggggggatgtgagttcctttgaagcctattttaaatccgaccccattgttgacgagcgctatgaactgcaaacccaggtggctaatctgcgcaatcgtttaaaacagtacgatggccaatattacggaattgggcactcaccggcgtacaaacgcatttgtgaaaccattgataaagccgctcgtggtaaagttagcgtgctgttgctgggtgagaccggagtaggaaaagaagtgattgcgcgtagtgtgcatttacgtagcgaacgtgcggaacagccgtttgttgcggtcaactgcgcggccattcctccggatctgattgaaagtgaactgtttggtgtggataaaggggcctataccggtgcggtcaatgctcgtgcgggccgctttgagcgcgccaacggtggtaccattttcctggatgaagttattgaactcaccccgcgcgcccaagccacccttctgcgtgttctccaggaaggagagctggaacgtgttggtggtgatcgcacccgtaaggttgatgtgcgtctgatcacggcaaccaacgagaatctggaagaagcggttaaaatggggcgctttcgtgcagatctgtttttccgcttaaacgtcttcccggtccaaatcccgccactgcgtgaacgcgtggaagacattcccttactggtggaacattttttacgccgtcatcacaaagagtatggtaagaaaacgttaggtttatcagatcgcgcaatggaagcgtgcctgcactatcagtggcctgggaacatccgcgagcttgaaaatgcgctggaacgtggggtgatcctgaccgaatcaaatgaaagcattaacgttgaatccctctttcccggcctggcaatggcaactgaaggggaccgcctttcctcggaagggcgtctggaagaggaatctggtgattcatggtttcgccagattattgatcagggtgtctcattagaagatctggaggcaggtctgatgcgtacagcgatggatcgctgcggccaaaacatttctcaggcggcgcgccttctcggccttacgcgcccggcgatggcataccgcctgaaaaaacttgatccgtcgctgagcgtgaaagccactggccgctgataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcccgggaaagcgcgatgaaccttttttatcgctgccttgatcaaatcgacaggtggttatgcgcgattgatgatttgctcaaatacagccagcgtgctgtagattttctctcataccccccctttcttttttacaaagaaaatcaataatttagatgaaataaggggatcggtataagcaatggcatggcggttgctagctatacgagacttaaaataaaaatagtggtgacccttcaatgttgtattttctcaactctgttcagattggttgctttcgccatgtatatcctcaaagcgggccagccgtagccgttacgctactagagattaaagaggagaaatactagatgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z