BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1710 1 RBS range1710 1 7 10 annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 BBa_K861171 1 Pcar Pcar, synthetic promoter repressed by CRP 2012-09-06T11:00:00Z 2015-05-08T01:13:36Z Escherichia coli str. K-12 substr. MG1655 This is a promoter designed for the Eschaerichia coli . It includes the CRP-binding site and the RNA polymerase-binding site which have a overlap of several base pairs.So because of the steric hindrance between CRP and RNA polymerase,gene downstream of the promoter will be repressed at high concentration of CRP.In the cells ,low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream.On the contrary,high glucose concentration will result in the expression of the promoter. false false _1121_ 0 12357 9 In stock true None false Kuanwei Sheng annotation2183482 1 Consensus CRP binding site range2183482 1 15 36 BBa_K1838008 1 BBa_K1838008 Glucose Inducible Blue Chromoprotein Construct 2015-09-17T11:00:00Z 2016-01-21T03:44:48Z The iGEM Registry (see component parts) This composite part features the glucose inducible promoter BBa_K861171 combined via 3A assembly with a blue chromoprotein+RBS part (BBa_K1073021). This part is meant to be placed into the tri-color glucose detection composite part in order to contribute increasing levels of blue protein as glucose concentration rises. false false _2264_ 4206 26050 9 false Inducible was very small so we had to synthesize over using 3A assembly. I would recommend finding some way to extend the length of one part or circumvent using parts <150 bp for 3A if a similar design is required for your new part/project. false Connor McFadden component2473541 1 BBa_K861171 component2473547 1 BBa_K1073021 annotation2473541 1 BBa_K861171 range2473541 1 1 36 annotation2473547 1 BBa_K1073021 range2473547 1 45 762 BBa_K864401 1 aeBlue aeBlue blue chromoprotein 2012-09-24T11:00:00Z 2015-05-08T01:13:37Z Source aeBlue false false _1124_ 0 9827 9 It's complicated true Synthesized and codon optimized for E. coli by Bioneer Corp. false Erik Lundin annotation2197206 1 aeBlue range2197206 1 1 699 BBa_K1073021 1 BBa_K1073021 Chromoprotein aeBlue with RBS 2013-09-16T11:00:00Z 2015-05-08T01:09:01Z This part is a combination of BBa_B0032 and BBa_K864401, which were taken from the 2013 distribution kit and the iGEM Team Uppsala. This part is a subpart of BBa_K107320. It does not include the promoter, if the user wants to add an e.g. inducable or stronger/weaker promoter. false false _1382_ 0 15674 9 In stock false no considerations. false iGEM Team Braunschweig 2013 component2349383 1 BBa_K864401 component2349380 1 BBa_B0032 annotation2349383 1 BBa_K864401 range2349383 1 20 718 annotation2349380 1 BBa_B0032 range2349380 1 1 13 BBa_K1838008_sequence 1 ttgacagctagctcaaatgtgatttaaatcacattttactagagtcacacaggaaagtactagatggcttcactggttaaaaaagacatgtgcatcaaaatgacgatggaaggaacagtaaacggtcaccatttcaagtgtgtaggagaaggcgaaggcaaaccatttgaagggacccaggtggaaaagatacgcatcactgaaggtgggcccttaccatttgcgtatgatattttggccccttgttgcatgtatggcagtaaaaccttcattaagcatgtgtcgggtattccggattactttaaggagtcttttcctgagggctttacctgggaaagaacacaaatcttcgaggatggcggctatctcaccatacaccaggacacgagccttcagggtaataattttattttcaaagttaatgtcatcggtgccaacttccctgcaaacggtcccgtgatgcagaaaaaaacagctggatgggaaccgtgcgttgagatgctttatccgcgggacggcgtcctgtgtggtcagagcctgatggccctgaaatgcactgatggcaatcatctgacgtcccacctgcgcactacctatcgttctcgcaagccatccaatgcagttaacatgccggaatttcattttggggatcatcgcattgagattttgaaagctgaacaaggtaaattttatgaacaatacgagtcagcggtggcccgttactgtgaggcggcaccgagtaaattagggcatcactaataa BBa_K861171_sequence 1 ttgacagctagctcaaatgtgatttaaatcacattt BBa_B0032_sequence 1 tcacacaggaaag BBa_K1073021_sequence 1 tcacacaggaaagtactagatggcttcactggttaaaaaagacatgtgcatcaaaatgacgatggaaggaacagtaaacggtcaccatttcaagtgtgtaggagaaggcgaaggcaaaccatttgaagggacccaggtggaaaagatacgcatcactgaaggtgggcccttaccatttgcgtatgatattttggccccttgttgcatgtatggcagtaaaaccttcattaagcatgtgtcgggtattccggattactttaaggagtcttttcctgagggctttacctgggaaagaacacaaatcttcgaggatggcggctatctcaccatacaccaggacacgagccttcagggtaataattttattttcaaagttaatgtcatcggtgccaacttccctgcaaacggtcccgtgatgcagaaaaaaacagctggatgggaaccgtgcgttgagatgctttatccgcgggacggcgtcctgtgtggtcagagcctgatggccctgaaatgcactgatggcaatcatctgacgtcccacctgcgcactacctatcgttctcgcaagccatccaatgcagttaacatgccggaatttcattttggggatcatcgcattgagattttgaaagctgaacaaggtaaattttatgaacaatacgagtcagcggtggcccgttactgtgaggcggcaccgagtaaattagggcatcactaataa BBa_K864401_sequence 1 atggcttcactggttaaaaaagacatgtgcatcaaaatgacgatggaaggaacagtaaacggtcaccatttcaagtgtgtaggagaaggcgaaggcaaaccatttgaagggacccaggtggaaaagatacgcatcactgaaggtgggcccttaccatttgcgtatgatattttggccccttgttgcatgtatggcagtaaaaccttcattaagcatgtgtcgggtattccggattactttaaggagtcttttcctgagggctttacctgggaaagaacacaaatcttcgaggatggcggctatctcaccatacaccaggacacgagccttcagggtaataattttattttcaaagttaatgtcatcggtgccaacttccctgcaaacggtcccgtgatgcagaaaaaaacagctggatgggaaccgtgcgttgagatgctttatccgcgggacggcgtcctgtgtggtcagagcctgatggccctgaaatgcactgatggcaatcatctgacgtcccacctgcgcactacctatcgttctcgcaagccatccaatgcagttaacatgccggaatttcattttggggatcatcgcattgagattttgaaagctgaacaaggtaaattttatgaacaatacgagtcagcggtggcccgttactgtgaggcggcaccgagtaaattagggcatcactaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z