BBa_K1842000 1 BBa_K1842000 LuxABfrp 2015-08-16T11:00:00Z 2015-08-17T06:07:42Z The luciferase used is a synthetic construct, modified from Photorhabdus Luminescens luciferase and engineered for enhanced activity and thermostability. The NADPH-flavin oxidoreductase used was isolated from Vibrio Harveyi. The part codes for a synthetic luciferase and a NADPH-flavin oxidoreductase, joined by a 2A peptide sequence. Genes used are codon optimized for expression in yeast. When provided with exogenous substrate or coexpressed with the lux system fatty acid reductase complex, the part allows for light production. Luciferase catalyzes the conversion of substrate for light production, while the oxidoreductase regenerates FMNH2 for use by luciferase. The 2A peptide allows for stoichiometric expression of the two proteins. false false _2268_ 26185 26185 9 false The sequence was too long to be directly synthesized, so we had to synthesize it in fragments that were joined through Gibson Assembly. false Walter Thavarajah annotation2435930 1 HIS range2435930 1 2926 2943 annotation2435926 1 P2A Linker range2435926 1 2140 2205 annotation2435927 1 MYC range2435927 1 2110 2139 annotation2435929 1 frp range2435929 1 2206 2925 annotation2435928 1 LuxAB Enhanced range2435928 1 1 2109 annotation2435931 1 Stop x2 range2435931 1 2944 2949 BBa_K1842000_sequence 1 atgggtaagttcggaaacttcctattgacctaccaaccaccacaattctctcagactgaggtcatgaagagattggtcaagttgggtagaatctcagaagagtgcggattcgatactgtttggttgttggaacaccacttcactgaatttggattgttgggtaacccatacgtcgccgcagcttacttgttgggtgctaccaagaagttaaacgtcggaaccgctgctattgtcttgccaaccgctcatccagttagacaactggaagacgttaatttattagaccaaatgtctaagggaaggtttagattcggtatctgtagaggtttatataacaaggacttcagagtcttcggaacagatatgaataactcccgtgctttggccgaatgttggtacggtttgattaagaacggtatgaccgaaggttacatggaagctgataacgaacacattaaattccacaaggtcaaggttaacccagctgcctactccagaggtggagccccggtctacgttgttgctgaatccgcttccactaccgaatgggccgctcaattcggtttgccaatgatcttgtcttggatcatcaacactaacgaaaagaaggcccaattggaattgtacaacgaagtcgctcaagaatacggtcacgacattcacaacattgatcactgcttgtcctacatcacctctgttgattacgactccattaaagccaaggaaatctgtcgtaagttcttgggtcactggtacgattcttacgtcaacgccaccactattttcgacgactccgaccaaaccagaggttatgattttaacaagggacagtggagagatttcgttttgaagggtcataaggacaccaacagaagaatcgattactcttacgaaatcaacccagtcggtaccccacaagaatgtatcgacatcattcaaaaagatatcgacgccaccggtatcagcaacgtctgctgcggtttcgaagctaatggtactgtcgatgaaatcgtcgcctctatgaagttgttccaatccgacgtcatgccatttttgaaggaaaagcagaggtcattgttgtacggcggaggtggttctggaggtggcggttctggtggtggaggttccatgaaatttggtttattctttcttaatttcattaattctacaaccgtccaagaacaatctatcgtcagaatgcaagaaattaccgaatacgttgataagttgaatttcgagcaaattttagtatacgaaaaccacttctccgataacggtgtcgttggtgctccattgaccgtctccggttttttacttggattgaccgaaaagatcaagattggttctttgaaccacatcatcaccacccatcacccagtcagaatcgccgaagaagcttgtttgttggatcaattgtctgaaggtagattcatcttgggtttctctgactgtgaaaagaaagacgagatgcacttcttcaacagaccagttgaataccaacaacaattgttcgaagaatgttacgaaattattaacgatgccttgactaccggttactgtaacccagataatgatttctactccttcccaaagatctccgtcaacccacacgcttacacgccaggtggtccaagaaagtacgttactgctacctcccaccatatcgtcgaatgggccgctaagaagggtattccattgattttcaagtgggacgatagcaacgacgttagatacgaatatgctgaaagatacaaggctgtcgccgataagtacgacgtcgatttgtctgaaatcgaccaccaattgatgattttggtcaactacaatgaagattcaaacaaggctaagcaagaaaccagagctttcatttccgattacgtcttggaaatgcacccaaacgaaaacttcgaaaacaagttggaagaaatcatcgctgaaaacgccgtgggtaactacactgaatgtattaccgctgctaagttggctattgaaaagtgtggtgccaagtccgtcttgttgtctttcgaaccaatgaatgacttgatgtctcaaaagaacgttattaacatcgtcgatgataacatcaaaaagtaccacatggaatacaccgaacaaaagttgatctctgaagaagatttgggttctggtgctactaatttttctttgttgaaacaagctggtgatgttgaagaaaatccaggtccaatgaacaacactatcgaaactatcttggctcatagatccatcagaaagttcaccgctgttccaattaccgacgaacaaagacaaactatcatccaagctggtttggccgcttcttcctcttccatgttgcaagtcgtttctattgtccgtgttaccgactctgaaaagagaaacgaattggctcaattcgccggtaaccaagcctacgtcgaatccgccgctgagttcttggtcttctgtatcgattaccaaagacacgctactattaacccagatgttcaagccgatttcaccgaattgactttgattggtgctgttgactctggtatcatggctcaaaactgtctattggcagccgaatccatgggtttaggtggtgtctacattggtggtttgcgtaactctgctgcccaagtcgatgaattgttgggtttgccagaaaactccgctgttttgttcggtatgtgcttgggtcaccctgatcaaaacccagaagtcaagccaagattgccagctcacgttgtcgttcacgaaaaccaataccaagaattgaacttggacgatatccaatcttacgaccaaaccatgcaagcttactacgcctctaggacctctaaccaaaagttgtctacttggtcccaagaagtcaccggtaagttggctggtgaatccagaccacacattttgccatacttgaactccaagggtttggccaagagacaccatcatcaccatcattaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z