BBa_K1842001 1 BBa_K1842001 LuxCDEC 2015-08-16T11:00:00Z 2015-08-17T05:38:26Z Each of the genes used was derived from the marine bioluminescent bacterium Photobacterium Phosphoreum. The part codes for the fatty acid reductase complex of the bacterial lux system from Photobacterium Phosphoreum. The system recruits fatty acids and converts them into aldehyde to be used as substrate in the system's light producing reaction. The genes coding for each subunit of the complex are separated by a P2A sequence for stoichiometric expression. The part is comprised of three unique genes - LuxC, LuxD, and LuxE, coding for a reductase, transferase, and synthetase, respectively. A duplicate copy of LuxC is present, resulting in its overexpression. Genes used are codon optimized for expression in yeast. false false _2268_ 26185 26185 9 false The coding sequence was too large to by synthesized in one piece, so it was instead synthesized in a series of fragments that were assembled via Gibson Assembly. false Walter Thavarajah annotation2435921 1 HA range2435921 1 3670 3702 annotation2435914 1 LuxC range2435914 1 1 1434 annotation2435924 1 FLAG range2435924 1 5203 5226 annotation2435918 1 V5 range2435918 1 2443 2484 annotation2435922 1 P2A Linker range2435922 1 3703 3768 annotation2435919 1 P2A Linker range2435919 1 2485 2550 annotation2435925 1 Stop x2 range2435925 1 5227 5232 annotation2435917 1 LuxD range2435917 1 1525 2442 annotation2435923 1 LuxC (duplicate) range2435923 1 3769 5202 annotation2435916 1 P2A Linker range2435916 1 1459 1524 annotation2435920 1 LuxE range2435920 1 2551 3669 annotation2435915 1 FLAG range2435915 1 1435 1458 BBa_K1842001_sequence 1 atgatcaagaagatcccaatgatcattggtggtggtgaaagagatacttccgaacacgaataccgtgaattgaccttgaactcctacaaggtctctatcccaatcatcaaccaagatgatgttgaagccatcaagtcccaaaacgtcgaaaacaacttgaacattaaccaaattgttaacttcttgtacactgtcggtcaaaagtggaagtccgaaaactactcaagaagattgacctacatcagagacttggtcagatttttgggttactccccagaaatggctaagttggaagccaactggatctctatgattttgtcttccaagtctgccttgtacgacatcgtcgaaactgaattaggttctcgtcacattgtcgacgaatggttgccacaaggtgattgttacgttaaagctatgcctaagggtaagtccgtccacttgttggctggtaacgtcccattgtccggtgttacctctatcatcagagctatcttgaccaagaacgaatgtatcatcaagacctcctctgctgatccattcaccgccattgctttggcctcttccttcatcgatactgacgaacaccacccaatttccagatctatgtccgtcatgtactggtcccacaacgaagatatcgctattccacaacaaatcatgaactgtgctgatgttgtcgtctcctggggtggttacgacgccatcaagtgggctaccgaacataccccagttaacgtcgacattttgaaattcggtccaaagaaatctatcgctatcgttgataacccagtcgacatcaccgcttctgctatcggtgtcgctcacgatatttgtttctacgatcaacaagcctgtttctctactcaagacatctactacattggtgacaacatcgacgctttcttcgacgaattggtcgaacaattgaacttgtacatggacatcctacctaaaggtgatcaaaccttcgacgaaaaggcttccttctctttgattgaaaaggaatgtcaattcgctaagtataaggttgaaaagggtgataaccaatcctggttgttggtcaagtccccattaggttctttcggtaaccaaccattggctagatccgcctacatccaccacgtttctgacatttccgaaatcactccatacatcgaaaacagaatcacccaaaccgttaccgttactccatgggaatcctctttcaaataccgtgatgtcttggcttctcacggtgctgaaagaatcgtcgaatccggtatgaacaacatcttcagagtcggtggtgctcacgacggtatgcgtccattgcaaagattggttaagtacatctctcatgaaagaccatacacctacactactaaggacgtcgccgttaagatcgaacaaaccagatacttggaagaagataagttcttggtcttcgtccctgattacaaagacgatgacgacaaaggtagcggtgctaccaacttctctttgttgaagcaagccggtgacgttgaagaaaacccaggcccaatgaagtccgaaaacaactctgtcccaatcgaccacgtcatcaaagttgacaacgaaagacacattagagtctgggaaaccttcccaaagaaccaatgtgataagagaaataacactatcgttatcgcttccggtttcgctagaagaatggatcacttcgccggtttggctgaatacttgtccactaacggtttccacgtcattagatacgactccttgaaccacgttggtttgtcttccggtgaaatcgaccaattctctatgtctgtcggtaagaagtctttgttgactgttattgattggttaaagtctgaacacggtatcgaccaagtcggtttaattgcttcctccttgtctgctagaattgcctatgacatcgtcgctgatgtcaacttgtctttcttgatcaccgctgtcggtgttgtcaacttgagaaacactttggaacaagctttgaagtacgactacttgcaaatggaaatcgatgaaattccagaagatttggatttcgacggttacaacttgggttccaaggtcttcgttactgattgtttcgaaaacaactgggacaccttggattccactatcaacaagactaagaacttgaacgtcccattcattgctttcgtcgccaacgacgactcctgggttcaacaacacgaagttgaagaattgatgtccaacatcaactctgacaagactaagatttactctttaatcggttcctctcacgatttaggcgaaaacttgattgtcttgagaaacttctaccaatccatcaccaaggctgctattgccttggactctaacttggtcggtttggttagcgaaatcatcgaaccacaattcgaagctttgactattgctaccgtcaacgaaagaagattgaagaacaagatccaatctaagtccttggctggtaagccaattccaaacccattgttgggtttagattccactggttcaggagctactaatttctcattgttaaagcaggctggagacgtcgaagagaatcctggtcctatgactattactttagacacctgtgaaaaggacatcatcgttagcactgaaatcgatgatattattttcacctcctctccattggacatcacttacgacgaacaagaaagaattaagcacaaattgatcttggaatccttcagataccactacaacaacaacgaagattacaagtctttctgtaacactcaaggtgtcgacgaaaacatttcctccttggacgacatcccagtctttccaacctccatgttcaagtacgctaagatctgtaccgctgatgaatctaacattgaaaactggttcacttcctccggtacctccggtgttaagtctcatatcgccagagacagagtctccatcgaacgtttgttgggttccgttaactacggtatgaagtacttgggttctttccacgaaaaccaattggaattggttaacatgggtccagacagattcaacgctaaaaacgtctggttcaagtacgttatgagcttggtcgagttattgtacccaaccactttcaccgttaacaacgacgaaatcgactttgaattgactatcaagtccttgaaggaaatctacaacaagggtaagggtatttgtttaatcggtccaccatacttcatttacttgttgtgtcaatacatgaaggaaaacgacatcgaatttaacgccggtaacagaatcttcatcatcaccggtggtggttggaagactaagcaaaagcaagctttaaatagacaagatttcaaccaattgttaatggaaaccttccatttggctcacgaatctcaaatcagagatactttcaaccaagtcgaattgaacacttgtttcttcgaagacaacagacaaagaaagcacgtcccaccatgggtctacgctagagctttggacccagtcaccttaaagcctgttgaagatggtcaagaaggtttaatttcctacatggacgcctcttccacctcttacccaactttcatcgtcactgacgatatcggtatcattcacactatcaaggatccagacccatatcaaggtaccactattgacatcgtcagaagattgaacaccagagaacaaaagggttgttccctatctatggcttccggtttgaaaatggcttacccatacgatgtcccagactacgctggtagcggtgctaccaacttctctttgttgaagcaagccggtgacgttgaagaaaacccaggcccaatgatcaagaagatcccaatgatcattggtggtggtgaaagagatacttccgaacacgaataccgtgaattgaccttgaactcctacaaggtctctatcccaatcatcaaccaagatgatgttgaagccatcaagtcccaaaacgtcgaaaacaacttgaacattaaccaaattgttaacttcttgtacactgtcggtcaaaagtggaagtccgaaaactactcaagaagattgacctacatcagagacttggtcagatttttgggttactccccagaaatggctaagttggaagccaactggatctctatgattttgtcttccaagtctgccttgtacgacatcgtcgaaactgaattaggttctcgtcacattgtcgacgaatggttgccacaaggtgattgttacgttaaagctatgcctaagggtaagtccgtccacttgttggctggtaacgtcccattgtccggtgttacctctatcatcagagctatcttgaccaagaacgaatgtatcatcaagacctcctctgctgatccattcaccgccattgctttggcctcttccttcatcgatactgacgaacaccacccaatttccagatctatgtccgtcatgtactggtcccacaacgaagatatcgctattccacaacaaatcatgaactgtgctgatgttgtcgtctcctggggtggttacgacgccatcaagtgggctaccgaacataccccagttaacgtcgacattttgaaattcggtccaaagaaatctatcgctatcgttgataacccagtcgacatcaccgcttctgctatcggtgtcgctcacgatatttgtttctacgatcaacaagcctgtttctctactcaagacatctactacattggtgacaacatcgacgctttcttcgacgaattggtcgaacaattgaacttgtacatggacatcctacctaaaggtgatcaaaccttcgacgaaaaggcttccttctctttgattgaaaaggaatgtcaattcgctaagtataaggttgaaaagggtgataaccaatcctggttgttggtcaagtccccattaggttctttcggtaaccaaccattggctagatccgcctacatccaccacgtttctgacatttccgaaatcactccatacatcgaaaacagaatcacccaaaccgttaccgttactccatgggaatcctctttcaaataccgtgatgtcttggcttctcacggtgctgaaagaatcgtcgaatccggtatgaacaacatcttcagagtcggtggtgctcacgacggtatgcgtccattgcaaagattggttaagtacatctctcatgaaagaccatacacctacactactaaggacgtcgccgttaagatcgaacaaaccagatacttggaagaagataagttcttggtcttcgtccctgattacaaagacgatgacgacaaataatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z