BBa_K1842002 1 BBa_K1842002 LuxCDED 2015-08-16T11:00:00Z 2015-08-17T06:19:14Z Each of the genes used was derived from the marine bioluminescent bacterium Photobacterium Phosphoreum. The part codes for the fatty acid reductase complex of the bacterial lux system from Photobacterium Phosphoreum. The system recruits fatty acids and converts them into aldehyde to be used as substrate in the system's light producing reaction. The genes coding for each subunit of the complex are separated by a P2A sequence for stoichiometric expression. The part is comprised of three unique genes - LuxC, LuxD, and LuxE, coding for a reductase, transferase, and synthetase, respectively. Each gene has an attached antibody tag - LuxC has a FLAG tag, LuxD has a V5 tag, and LuxE has a HA tag. A duplicate copy of LuxD is present, resulting in its overexpression. Genes used are codon optimized for expression in yeast. false false _2268_ 26185 26185 9 false The coding sequence was too large to be synthesized in one piece, so it was instead synthesized in a series of fragments that were assembled via Gibson Assembly. false Walter Thavarajah annotation2435933 1 FLAG range2435933 1 1435 1458 annotation2435937 1 P2A Linker range2435937 1 2485 2550 annotation2435935 1 LuxD range2435935 1 1525 2442 annotation2435941 1 LuxD (Duplicate) range2435941 1 3769 4686 annotation2435940 1 P2A Linker range2435940 1 3703 3768 annotation2435932 1 LuxC range2435932 1 1 1434 annotation2435934 1 P2A Linker range2435934 1 1459 1524 annotation2435939 1 HA range2435939 1 3670 3702 annotation2435942 1 V5 range2435942 1 4687 4728 annotation2435943 1 Stop x2 range2435943 1 4729 4734 annotation2435938 1 LuxE range2435938 1 2551 3669 annotation2435936 1 V5 range2435936 1 2443 2484 BBa_K1842002_sequence 1 atgatcaagaagatcccaatgatcattggtggtggtgaaagagatacttccgaacacgaataccgtgaattgaccttgaactcctacaaggtctctatcccaatcatcaaccaagatgatgttgaagccatcaagtcccaaaacgtcgaaaacaacttgaacattaaccaaattgttaacttcttgtacactgtcggtcaaaagtggaagtccgaaaactactcaagaagattgacctacatcagagacttggtcagatttttgggttactccccagaaatggctaagttggaagccaactggatctctatgattttgtcttccaagtctgccttgtacgacatcgtcgaaactgaattaggttctcgtcacattgtcgacgaatggttgccacaaggtgattgttacgttaaagctatgcctaagggtaagtccgtccacttgttggctggtaacgtcccattgtccggtgttacctctatcatcagagctatcttgaccaagaacgaatgtatcatcaagacctcctctgctgatccattcaccgccattgctttggcctcttccttcatcgatactgacgaacaccacccaatttccagatctatgtccgtcatgtactggtcccacaacgaagatatcgctattccacaacaaatcatgaactgtgctgatgttgtcgtctcctggggtggttacgacgccatcaagtgggctaccgaacataccccagttaacgtcgacattttgaaattcggtccaaagaaatctatcgctatcgttgataacccagtcgacatcaccgcttctgctatcggtgtcgctcacgatatttgtttctacgatcaacaagcctgtttctctactcaagacatctactacattggtgacaacatcgacgctttcttcgacgaattggtcgaacaattgaacttgtacatggacatcctacctaaaggtgatcaaaccttcgacgaaaaggcttccttctctttgattgaaaaggaatgtcaattcgctaagtataaggttgaaaagggtgataaccaatcctggttgttggtcaagtccccattaggttctttcggtaaccaaccattggctagatccgcctacatccaccacgtttctgacatttccgaaatcactccatacatcgaaaacagaatcacccaaaccgttaccgttactccatgggaatcctctttcaaataccgtgatgtcttggcttctcacggtgctgaaagaatcgtcgaatccggtatgaacaacatcttcagagtcggtggtgctcacgacggtatgcgtccattgcaaagattggttaagtacatctctcatgaaagaccatacacctacactactaaggacgtcgccgttaagatcgaacaaaccagatacttggaagaagataagttcttggtcttcgtccctgattacaaagacgatgacgacaaaggtagcggtgctaccaacttctctttgttgaagcaagccggtgacgttgaagaaaacccaggcccaatgaagtccgaaaacaactctgtcccaatcgaccacgtcatcaaagttgacaacgaaagacacattagagtctgggaaaccttcccaaagaaccaatgtgataagagaaataacactatcgttatcgcttccggtttcgctagaagaatggatcacttcgccggtttggctgaatacttgtccactaacggtttccacgtcattagatacgactccttgaaccacgttggtttgtcttccggtgaaatcgaccaattctctatgtctgtcggtaagaagtctttgttgactgttattgattggttaaagtctgaacacggtatcgaccaagtcggtttaattgcttcctccttgtctgctagaattgcctatgacatcgtcgctgatgtcaacttgtctttcttgatcaccgctgtcggtgttgtcaacttgagaaacactttggaacaagctttgaagtacgactacttgcaaatggaaatcgatgaaattccagaagatttggatttcgacggttacaacttgggttccaaggtcttcgttactgattgtttcgaaaacaactgggacaccttggattccactatcaacaagactaagaacttgaacgtcccattcattgctttcgtcgccaacgacgactcctgggttcaacaacacgaagttgaagaattgatgtccaacatcaactctgacaagactaagatttactctttaatcggttcctctcacgatttaggcgaaaacttgattgtcttgagaaacttctaccaatccatcaccaaggctgctattgccttggactctaacttggtcggtttggttagcgaaatcatcgaaccacaattcgaagctttgactattgctaccgtcaacgaaagaagattgaagaacaagatccaatctaagtccttggctggtaagccaattccaaacccattgttgggtttagattccactggttcaggagctactaatttctcattgttaaagcaggctggagacgtcgaagagaatcctggtcctatgactattactttagacacctgtgaaaaggacatcatcgttagcactgaaatcgatgatattattttcacctcctctccattggacatcacttacgacgaacaagaaagaattaagcacaaattgatcttggaatccttcagataccactacaacaacaacgaagattacaagtctttctgtaacactcaaggtgtcgacgaaaacatttcctccttggacgacatcccagtctttccaacctccatgttcaagtacgctaagatctgtaccgctgatgaatctaacattgaaaactggttcacttcctccggtacctccggtgttaagtctcatatcgccagagacagagtctccatcgaacgtttgttgggttccgttaactacggtatgaagtacttgggttctttccacgaaaaccaattggaattggttaacatgggtccagacagattcaacgctaaaaacgtctggttcaagtacgttatgagcttggtcgagttattgtacccaaccactttcaccgttaacaacgacgaaatcgactttgaattgactatcaagtccttgaaggaaatctacaacaagggtaagggtatttgtttaatcggtccaccatacttcatttacttgttgtgtcaatacatgaaggaaaacgacatcgaatttaacgccggtaacagaatcttcatcatcaccggtggtggttggaagactaagcaaaagcaagctttaaatagacaagatttcaaccaattgttaatggaaaccttccatttggctcacgaatctcaaatcagagatactttcaaccaagtcgaattgaacacttgtttcttcgaagacaacagacaaagaaagcacgtcccaccatgggtctacgctagagctttggacccagtcaccttaaagcctgttgaagatggtcaagaaggtttaatttcctacatggacgcctcttccacctcttacccaactttcatcgtcactgacgatatcggtatcattcacactatcaaggatccagacccatatcaaggtaccactattgacatcgtcagaagattgaacaccagagaacaaaagggttgttccctatctatggcttccggtttgaaaatggcttacccatacgatgtcccagactacgctggtagcggtgctaccaacttctctttgttgaagcaagccggtgacgttgaagaaaacccaggcccaatgaagtccgaaaacaactctgtcccaatcgaccacgtcatcaaagttgacaacgaaagacacattagagtctgggaaaccttcccaaagaaccaatgtgataagagaaataacactatcgttatcgcttccggtttcgctagaagaatggatcacttcgccggtttggctgaatacttgtccactaacggtttccacgtcattagatacgactccttgaaccacgttggtttgtcttccggtgaaatcgaccaattctctatgtctgtcggtaagaagtctttgttgactgttattgattggttaaagtctgaacacggtatcgaccaagtcggtttaattgcttcctccttgtctgctagaattgcctatgacatcgtcgctgatgtcaacttgtctttcttgatcaccgctgtcggtgttgtcaacttgagaaacactttggaacaagctttgaagtacgactacttgcaaatggaaatcgatgaaattccagaagatttggatttcgacggttacaacttgggttccaaggtcttcgttactgattgtttcgaaaacaactgggacaccttggattccactatcaacaagactaagaacttgaacgtcccattcattgctttcgtcgccaacgacgactcctgggttcaacaacacgaagttgaagaattgatgtccaacatcaactctgacaagactaagatttactctttaatcggttcctctcacgatttaggcgaaaacttgattgtcttgagaaacttctaccaatccatcaccaaggctgctattgccttggactctaacttggtcggtttggttagcgaaatcatcgaaccacaattcgaagctttgactattgctaccgtcaacgaaagaagattgaagaacaagatccaatctaagtccttggctggtaagccaattccaaacccattgttgggtttagattccacttaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z