BBa_K1842003 1 BBa_K1842003 LuxCDEE 2015-08-16T11:00:00Z 2015-08-17T06:32:01Z Each of the genes used was derived from the marine bioluminescent bacterium Photobacterium Phosphoreum. The part codes for the fatty acid reductase complex of the bacterial lux system from Photobacterium Phosphoreum. The system recruits fatty acids and converts them into aldehyde to be used as substrate in the system's light producing reaction. The genes coding for each subunit of the complex are separated by a P2A sequence for stoichiometric expression. The part is comprised of three unique genes - LuxC, LuxD, and LuxE, coding for a reductase, transferase, and synthetase, respectively. Each gene has an attached antibody tag - LuxC has a FLAG tag, LuxD has a V5 tag, and LuxE has a HA tag. A duplicate copy of LuxE is present, resulting in its overexpression. Genes used are codon optimized for expression in yeast. false false _2268_ 26185 26185 9 false The coding sequence was too large to be synthesized in one piece, so it was instead synthesized in a series of fragments that were assembled via Gibson Assembly. false Walter Thavarajah annotation2435945 1 FLAG range2435945 1 1435 1458 annotation2435961 1 LuxD range2435961 1 1525 2442 annotation2435962 1 V5 range2435962 1 2443 2484 annotation2435983 1 Stop x2 range2435983 1 4921 4926 annotation2435946 1 P2A Linker range2435946 1 1459 1524 annotation2435963 1 P2A Linker range2435963 1 2485 2550 annotation2435979 1 HA range2435979 1 3670 3702 annotation2435944 1 LuxC range2435944 1 1 1434 annotation2435981 1 LuxE (duplicate) range2435981 1 3769 4887 annotation2435982 1 HA range2435982 1 4888 4920 annotation2435964 1 LuxE range2435964 1 2551 3669 annotation2435980 1 P2A Linker range2435980 1 3703 3768 BBa_K1842003_sequence 1 atgatcaagaagatcccaatgatcattggtggtggtgaaagagatacttccgaacacgaataccgtgaattgaccttgaactcctacaaggtctctatcccaatcatcaaccaagatgatgttgaagccatcaagtcccaaaacgtcgaaaacaacttgaacattaaccaaattgttaacttcttgtacactgtcggtcaaaagtggaagtccgaaaactactcaagaagattgacctacatcagagacttggtcagatttttgggttactccccagaaatggctaagttggaagccaactggatctctatgattttgtcttccaagtctgccttgtacgacatcgtcgaaactgaattaggttctcgtcacattgtcgacgaatggttgccacaaggtgattgttacgttaaagctatgcctaagggtaagtccgtccacttgttggctggtaacgtcccattgtccggtgttacctctatcatcagagctatcttgaccaagaacgaatgtatcatcaagacctcctctgctgatccattcaccgccattgctttggcctcttccttcatcgatactgacgaacaccacccaatttccagatctatgtccgtcatgtactggtcccacaacgaagatatcgctattccacaacaaatcatgaactgtgctgatgttgtcgtctcctggggtggttacgacgccatcaagtgggctaccgaacataccccagttaacgtcgacattttgaaattcggtccaaagaaatctatcgctatcgttgataacccagtcgacatcaccgcttctgctatcggtgtcgctcacgatatttgtttctacgatcaacaagcctgtttctctactcaagacatctactacattggtgacaacatcgacgctttcttcgacgaattggtcgaacaattgaacttgtacatggacatcctacctaaaggtgatcaaaccttcgacgaaaaggcttccttctctttgattgaaaaggaatgtcaattcgctaagtataaggttgaaaagggtgataaccaatcctggttgttggtcaagtccccattaggttctttcggtaaccaaccattggctagatccgcctacatccaccacgtttctgacatttccgaaatcactccatacatcgaaaacagaatcacccaaaccgttaccgttactccatgggaatcctctttcaaataccgtgatgtcttggcttctcacggtgctgaaagaatcgtcgaatccggtatgaacaacatcttcagagtcggtggtgctcacgacggtatgcgtccattgcaaagattggttaagtacatctctcatgaaagaccatacacctacactactaaggacgtcgccgttaagatcgaacaaaccagatacttggaagaagataagttcttggtcttcgtccctgattacaaagacgatgacgacaaaggtagcggtgctaccaacttctctttgttgaagcaagccggtgacgttgaagaaaacccaggcccaatgaagtccgaaaacaactctgtcccaatcgaccacgtcatcaaagttgacaacgaaagacacattagagtctgggaaaccttcccaaagaaccaatgtgataagagaaataacactatcgttatcgcttccggtttcgctagaagaatggatcacttcgccggtttggctgaatacttgtccactaacggtttccacgtcattagatacgactccttgaaccacgttggtttgtcttccggtgaaatcgaccaattctctatgtctgtcggtaagaagtctttgttgactgttattgattggttaaagtctgaacacggtatcgaccaagtcggtttaattgcttcctccttgtctgctagaattgcctatgacatcgtcgctgatgtcaacttgtctttcttgatcaccgctgtcggtgttgtcaacttgagaaacactttggaacaagctttgaagtacgactacttgcaaatggaaatcgatgaaattccagaagatttggatttcgacggttacaacttgggttccaaggtcttcgttactgattgtttcgaaaacaactgggacaccttggattccactatcaacaagactaagaacttgaacgtcccattcattgctttcgtcgccaacgacgactcctgggttcaacaacacgaagttgaagaattgatgtccaacatcaactctgacaagactaagatttactctttaatcggttcctctcacgatttaggcgaaaacttgattgtcttgagaaacttctaccaatccatcaccaaggctgctattgccttggactctaacttggtcggtttggttagcgaaatcatcgaaccacaattcgaagctttgactattgctaccgtcaacgaaagaagattgaagaacaagatccaatctaagtccttggctggtaagccaattccaaacccattgttgggtttagattccactggttcaggagctactaatttctcattgttaaagcaggctggagacgtcgaagagaatcctggtcctatgactattactttagacacctgtgaaaaggacatcatcgttagcactgaaatcgatgatattattttcacctcctctccattggacatcacttacgacgaacaagaaagaattaagcacaaattgatcttggaatccttcagataccactacaacaacaacgaagattacaagtctttctgtaacactcaaggtgtcgacgaaaacatttcctccttggacgacatcccagtctttccaacctccatgttcaagtacgctaagatctgtaccgctgatgaatctaacattgaaaactggttcacttcctccggtacctccggtgttaagtctcatatcgccagagacagagtctccatcgaacgtttgttgggttccgttaactacggtatgaagtacttgggttctttccacgaaaaccaattggaattggttaacatgggtccagacagattcaacgctaaaaacgtctggttcaagtacgttatgagcttggtcgagttattgtacccaaccactttcaccgttaacaacgacgaaatcgactttgaattgactatcaagtccttgaaggaaatctacaacaagggtaagggtatttgtttaatcggtccaccatacttcatttacttgttgtgtcaatacatgaaggaaaacgacatcgaatttaacgccggtaacagaatcttcatcatcaccggtggtggttggaagactaagcaaaagcaagctttaaatagacaagatttcaaccaattgttaatggaaaccttccatttggctcacgaatctcaaatcagagatactttcaaccaagtcgaattgaacacttgtttcttcgaagacaacagacaaagaaagcacgtcccaccatgggtctacgctagagctttggacccagtcaccttaaagcctgttgaagatggtcaagaaggtttaatttcctacatggacgcctcttccacctcttacccaactttcatcgtcactgacgatatcggtatcattcacactatcaaggatccagacccatatcaaggtaccactattgacatcgtcagaagattgaacaccagagaacaaaagggttgttccctatctatggcttccggtttgaaaatggcttacccatacgatgtcccagactacgctggtagcggtgctaccaacttctctttgttgaagcaagccggtgacgttgaagaaaacccaggcccaatgactattactttagacacctgtgaaaaggacatcatcgttagcactgaaatcgatgatattattttcacctcctctccattggacatcacttacgacgaacaagaaagaattaagcacaaattgatcttggaatccttcagataccactacaacaacaacgaagattacaagtctttctgtaacactcaaggtgtcgacgaaaacatttcctccttggacgacatcccagtctttccaacctccatgttcaagtacgctaagatctgtaccgctgatgaatctaacattgaaaactggttcacttcctccggtacctccggtgttaagtctcatatcgccagagacagagtctccatcgaacgtttgttgggttccgttaactacggtatgaagtacttgggttctttccacgaaaaccaattggaattggttaacatgggtccagacagattcaacgctaaaaacgtctggttcaagtacgttatgagcttggtcgagttattgtacccaaccactttcaccgttaacaacgacgaaatcgactttgaattgactatcaagtccttgaaggaaatctacaacaagggtaagggtatttgtttaatcggtccaccatacttcatttacttgttgtgtcaatacatgaaggaaaacgacatcgaatttaacgccggtaacagaatcttcatcatcaccggtggtggttggaagactaagcaaaagcaagctttaaatagacaagatttcaaccaattgttaatggaaaccttccatttggctcacgaatctcaaatcagagatactttcaaccaagtcgaattgaacacttgtttcttcgaagacaacagacaaagaaagcacgtcccaccatgggtctacgctagagctttggacccagtcaccttaaagcctgttgaagatggtcaagaaggtttaatttcctacatggacgcctcttccacctcttacccaactttcatcgtcactgacgatatcggtatcattcacactatcaaggatccagacccatatcaaggtaccactattgacatcgtcagaagattgaacaccagagaacaaaagggttgttccctatctatggcttccggtttgaaaatggcttacccatacgatgtcccagactacgcttaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z