BBa_K1842004 1 BBa_K1842004 LuxDEC 2015-09-16T11:00:00Z 2015-09-17T08:51:03Z Each of the genes used was derived from the marine bioluminescent bacterium Photobacterium Phosphoreum. The part codes for the fatty acid reductase complex of the bacterial lux system from Photobacterium Phosphoreum. The system recruits fatty acids and converts them into aldehyde to be used as substrate in the system's light producing reaction. The genes coding for each subunit of the complex are separated by a P2A sequence for stoichiometric expression. The part is comprised of three unique genes - LuxC, LuxD, and LuxE, coding for a reductase, transferase, and synthetase, respectively. Each gene has an attached antibody tag - LuxC has a FLAG tag, LuxD has a V5 tag, and LuxE has a HA tag. One copy of each gene is present. Genes used are codon optimized for expression in yeast. false false _2268_ 26185 26185 9 false The part was PCR amplified from BBa_K1842001. false Walter Thavarajah annotation2468010 1 Stop x2 range2468010 1 3703 3708 annotation2468009 1 FLAG Tag range2468009 1 3679 3702 annotation2468006 1 LuxC range2468006 1 2245 3678 annotation2467968 1 LuxD range2467968 1 1 918 annotation2467985 1 LuxE range2467985 1 1027 2145 annotation2467969 1 V5 Tag range2467969 1 919 960 annotation2467970 1 P2A range2467970 1 961 1026 annotation2467986 1 HA Tag range2467986 1 2146 2178 annotation2467987 1 P2A range2467987 1 2179 2244 BBa_K1842004_sequence 1 atgaagtccgaaaacaactctgtcccaatcgaccacgtcatcaaagttgacaacgaaagacacattagagtctgggaaaccttcccaaagaaccaatgtgataagagaaataacactatcgttatcgcttccggtttcgctagaagaatggatcacttcgccggtttggctgaatacttgtccactaacggtttccacgtcattagatacgactccttgaaccacgttggtttgtcttccggtgaaatcgaccaattctctatgtctgtcggtaagaagtctttgttgactgttattgattggttaaagtctgaacacggtatcgaccaagtcggtttaattgcttcctccttgtctgctagaattgcctatgacatcgtcgctgatgtcaacttgtctttcttgatcaccgctgtcggtgttgtcaacttgagaaacactttggaacaagctttgaagtacgactacttgcaaatggaaatcgatgaaattccagaagatttggatttcgacggttacaacttgggttccaaggtcttcgttactgattgtttcgaaaacaactgggacaccttggattccactatcaacaagactaagaacttgaacgtcccattcattgctttcgtcgccaacgacgactcctgggttcaacaacacgaagttgaagaattgatgtccaacatcaactctgacaagactaagatttactctttaatcggttcctctcacgatttaggcgaaaacttgattgtcttgagaaacttctaccaatccatcaccaaggctgctattgccttggactctaacttggtcggtttggttagcgaaatcatcgaaccacaattcgaagctttgactattgctaccgtcaacgaaagaagattgaagaacaagatccaatctaagtccttggctggtaagccaattccaaacccattgttgggtttagattccactggttcaggagctactaatttctcattgttaaagcaggctggagacgtcgaagagaatcctggtcctatgactattactttagacacctgtgaaaaggacatcatcgttagcactgaaatcgatgatattattttcacctcctctccattggacatcacttacgacgaacaagaaagaattaagcacaaattgatcttggaatccttcagataccactacaacaacaacgaagattacaagtctttctgtaacactcaaggtgtcgacgaaaacatttcctccttggacgacatcccagtctttccaacctccatgttcaagtacgctaagatctgtaccgctgatgaatctaacattgaaaactggttcacttcctccggtacctccggtgttaagtctcatatcgccagagacagagtctccatcgaacgtttgttgggttccgttaactacggtatgaagtacttgggttctttccacgaaaaccaattggaattggttaacatgggtccagacagattcaacgctaaaaacgtctggttcaagtacgttatgagcttggtcgagttattgtacccaaccactttcaccgttaacaacgacgaaatcgactttgaattgactatcaagtccttgaaggaaatctacaacaagggtaagggtatttgtttaatcggtccaccatacttcatttacttgttgtgtcaatacatgaaggaaaacgacatcgaatttaacgccggtaacagaatcttcatcatcaccggtggtggttggaagactaagcaaaagcaagctttaaatagacaagatttcaaccaattgttaatggaaaccttccatttggctcacgaatctcaaatcagagatactttcaaccaagtcgaattgaacacttgtttcttcgaagacaacagacaaagaaagcacgtcccaccatgggtctacgctagagctttggacccagtcaccttaaagcctgttgaagatggtcaagaaggtttaatttcctacatggacgcctcttccacctcttacccaactttcatcgtcactgacgatatcggtatcattcacactatcaaggatccagacccatatcaaggtaccactattgacatcgtcagaagattgaacaccagagaacaaaagggttgttccctatctatggcttccggtttgaaaatggcttacccatacgatgtcccagactacgctggtagcggtgctaccaacttctctttgttgaagcaagccggtgacgttgaagaaaacccaggcccaatgatcaagaagatcccaatgatcattggtggtggtgaaagagatacttccgaacacgaataccgtgaattgaccttgaactcctacaaggtctctatcccaatcatcaaccaagatgatgttgaagccatcaagtcccaaaacgtcgaaaacaacttgaacattaaccaaattgttaacttcttgtacactgtcggtcaaaagtggaagtccgaaaactactcaagaagattgacctacatcagagacttggtcagatttttgggttactccccagaaatggctaagttggaagccaactggatctctatgattttgtcttccaagtctgccttgtacgacatcgtcgaaactgaattaggttctcgtcacattgtcgacgaatggttgccacaaggtgattgttacgttaaagctatgcctaagggtaagtccgtccacttgttggctggtaacgtcccattgtccggtgttacctctatcatcagagctatcttgaccaagaacgaatgtatcatcaagacctcctctgctgatccattcaccgccattgctttggcctcttccttcatcgatactgacgaacaccacccaatttccagatctatgtccgtcatgtactggtcccacaacgaagatatcgctattccacaacaaatcatgaactgtgctgatgttgtcgtctcctggggtggttacgacgccatcaagtgggctaccgaacataccccagttaacgtcgacattttgaaattcggtccaaagaaatctatcgctatcgttgataacccagtcgacatcaccgcttctgctatcggtgtcgctcacgatatttgtttctacgatcaacaagcctgtttctctactcaagacatctactacattggtgacaacatcgacgctttcttcgacgaattggtcgaacaattgaacttgtacatggacatcctacctaaaggtgatcaaaccttcgacgaaaaggcttccttctctttgattgaaaaggaatgtcaattcgctaagtataaggttgaaaagggtgataaccaatcctggttgttggtcaagtccccattaggttctttcggtaaccaaccattggctagatccgcctacatccaccacgtttctgacatttccgaaatcactccatacatcgaaaacagaatcacccaaaccgttaccgttactccatgggaatcctctttcaaataccgtgatgtcttggcttctcacggtgctgaaagaatcgtcgaatccggtatgaacaacatcttcagagtcggtggtgctcacgacggtatgcgtccattgcaaagattggttaagtacatctctcatgaaagaccatacacctacactactaaggacgtcgccgttaagatcgaacaaaccagatacttggaagaagataagttcttggtcttcgtccctgattacaaagacgatgacgacaaataatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z