BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986785
1
-35
range1986785
1
20
25
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986787
1
-10
range1986787
1
43
48
annotation1986786
1
TetR 2
range1986786
1
26
44
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_S05278
1
BBa_S05278
B0034:K1846002
2015-08-27T11:00:00Z
2015-08-28T03:51:09Z
false
false
_9_
27305
27305
9
false
false
Barbara Steijl
annotation2439243
1
BBa_K1846002
range2439243
1
19
603
annotation2439241
1
conserved
range2439241
1
5
8
annotation2439242
1
BBa_B0034
range2439242
1
1
12
BBa_S05279
1
BBa_S05279
K1846002:B0010
2015-08-27T11:00:00Z
2015-08-28T03:52:59Z
false
false
_9_
27305
27305
9
false
false
Barbara Steijl
annotation2439262
1
BBa_B0010
range2439262
1
594
673
annotation2439261
1
BBa_K1846002
range2439261
1
1
585
annotation2439263
1
stem_loop
range2439263
1
605
648
BBa_S05276
1
BBa_S05276
R0040:B0034
2015-08-27T11:00:00Z
2015-08-28T03:23:28Z
false
false
_9_
27305
27305
9
false
false
Barbara Steijl
annotation2439176
1
conserved
range2439176
1
67
70
annotation2439177
1
BBa_B0034
range2439177
1
63
74
annotation2439174
1
TetR 2
range2439174
1
26
44
annotation2439173
1
-35
range2439173
1
20
25
annotation2439171
1
BBa_R0040
range2439171
1
1
54
annotation2439175
1
-10
range2439175
1
43
48
annotation2439172
1
TetR 1
range2439172
1
1
19
BBa_K1846001
1
tfa+
Bacteriophage lambda tail fibre assembly protein circuit
2015-08-25T11:00:00Z
2015-09-20T01:26:15Z
...
The tfa (tail fibre assembly) protein of bacteriophage Lambda assists in the assembly of the stf (short tail fibre) protein into a functional tail fibre. This part provides the gene sequence for tfa, together with a TetR repressible promoter (TetO, available separately as BioBrick part BBa_R0040), ribosome binding site (available separately as part BBa_B0034) and an rrNB T1 terminator (available separately as BioBrick part BBa_B0010).
false
false
_2272_
24377
27305
9
false
...
false
Luba Prout
component2439280
1
BBa_S05279
component2439277
1
BBa_S05278
component2439275
1
BBa_B0034
component2439279
1
BBa_K1846002
component2439264
1
BBa_R0040
component2439281
1
BBa_B0010
component2439278
1
BBa_K1846002
component2439272
1
BBa_S05276
component2439269
1
BBa_R0040
component2439274
1
BBa_B0034
annotation2439280
1
BBa_S05279
range2439280
1
684
720
annotation2439278
1
BBa_K1846002
range2439278
1
99
683
annotation2439281
1
BBa_B0010
range2439281
1
721
800
annotation2439275
1
BBa_B0034
range2439275
1
93
98
annotation2439272
1
BBa_S05276
range2439272
1
55
80
annotation2439277
1
BBa_S05278
range2439277
1
93
98
annotation2439279
1
BBa_K1846002
range2439279
1
684
720
annotation2439274
1
BBa_B0034
range2439274
1
81
92
annotation2439269
1
BBa_R0040
range2439269
1
55
80
annotation2439264
1
BBa_R0040
range2439264
1
1
54
BBa_K1846002
1
tfa
Bacteriophage lambda tail fibre assembly (tfa) protein
2015-08-25T11:00:00Z
2015-09-20T01:20:28Z
The sequence has been adapted from the genomic sequence of bacteriophage Lambda.
The tfa (tail fibre assembly) protein of bacteriophage Lambda assists in the assembly of the stf (short tail fibre) protein into a functional tail fibre.
false
false
_2272_
24377
27305
9
false
Sequence was optimised to remove illegal restriction sites.
false
Luba Prout
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_S05278_sequence
1
atacaa
BBa_K1846001_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgatgtcaaagaatacaagcattgactaaagaggagaaaatacaaatggcgttccgtatgagtgaacaaccacgtaccattaaaatttataatctgctggccggtactaatgaatttattggtgaaggtgacgcatatattccgcctcataccggcctgcctgcaaacagtaccgatattgcaccgccagatattccggctggctttgtggctgttttcaacagtgatgaggcatcgtggcatctcgttgaagaccatcgtggtaaaaccgtctatgacgtggcttccggcgacgcgttatttatttctgaactcggtccgttaccggaaaattttacctggttatcgccgggtggggaatatcagaagtggaacggcacagcctgggtgaaggatacggaagcagaaaaactgttccgtatccgtgaggcggaagaaacaaaaaaaagcctgatgcaagtagccagtgagcatattgcgccgcttcaggatgcggcggatctggaaattgcaacgaaggaagaaacctcgttgctggaagcctggaagaagtatcgtgtgttgctgaaccgtgttgatacatcaactgcacctgatattgagtggcctgctgtccctgttatggagtaataacgctgatactgatagtgctagtagtgtagatcgcccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_S05279_sequence
1
taacgctgatactgatagtgctagtagtgtagatcgc
BBa_S05276_sequence
1
gatgtcaaagaatacaagcattgact
BBa_K1846002_sequence
1
atggcgttccgtatgagtgaacaaccacgtaccattaaaatttataatctgctggccggtactaatgaatttattggtgaaggtgacgcatatattccgcctcataccggcctgcctgcaaacagtaccgatattgcaccgccagatattccggctggctttgtggctgttttcaacagtgatgaggcatcgtggcatctcgttgaagaccatcgtggtaaaaccgtctatgacgtggcttccggcgacgcgttatttatttctgaactcggtccgttaccggaaaattttacctggttatcgccgggtggggaatatcagaagtggaacggcacagcctgggtgaaggatacggaagcagaaaaactgttccgtatccgtgaggcggaagaaacaaaaaaaagcctgatgcaagtagccagtgagcatattgcgccgcttcaggatgcggcggatctggaaattgcaacgaaggaagaaacctcgttgctggaagcctggaagaagtatcgtgtgttgctgaaccgtgttgatacatcaactgcacctgatattgagtggcctgctgtccctgttatggagtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z