LOCUS       BBa_K1846003             888 bp    DNA     linear       22-Nov-2024
DEFINITION  TetR circuit with constitutive promoter
ACCESSION   BBa_K1846003
VERSION     BBa_K1846003.1  
FEATURES             Location/Qualifiers
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=P(Cat)
     promoter        1..38
                     /label=BBa_I14033
     misc_feature    53..56
                     /label=conserved
     RBS             47..60
                     /label=BBa_B0031
     CDS             70..686
                     /label=tetR
     misc_feature    687..720
                     /label=SsrA
     misc_feature    727..751
                     /label=Help:Barcodes
     CDS             67..726
                     /label=BBa_C0040
     stem_loop       771..814
                     /label=stem_loop
     misc_feature    760..839
                     /label=BBa_B0010
     terminator      760..839
                     /label=BBa_B0010
     stem_loop       855..874
                     /label=T7 TE
     misc_feature    881..881
                     /label=stop
     polyA_site      875..888
                     /label=polya
     misc_feature    848..888
                     /label=BBa_B0012
     terminator      848..888
                     /label=BBa_B0012
     misc_feature    47..888
                     /label=BBa_P0140
ORIGIN
        1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagtcac acaggaaacc
       61 tactagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat
      121 gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag
      181 cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag
      241 atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt
      301 ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa
      361 gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc
      421 tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg
      481 cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg
      541 gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat
      601 caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa
      661 aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct
      721 taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa
      781 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      841 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//