BBa_K1846004 1 stf stf (short tail fibre) gene of bacteriophage lambda 2015-09-16T11:00:00Z 2015-09-19T11:59:35Z ... The stf gene encodes the short tail fibres found on the wild-type strain of bacteriophage lambda. The gene has been inactivated by a single frameshift mutation in the lab strain most commonly used in research (λ-PaPa). This gene has been created using the ORF-314 and ORF-401, cloning the two open reading frames into a single in-frame coding sequence through the use of an Eco-RV restriction site. The C-terminal end of the stf protein offers host receptor recognition for OmpC on the surface of E.coli, widening the host range of λ-PaPa, which recognises only the lamB protein through its tip attachment protein. Additionally, this gene displays a high level (~40%) of homology with the gp37 of bacteriophage T4. false false _2272_ 24377 27305 9 false The gene was optimised to remove illegal restriction sites to make the sequence BioBrick-compatible. false Luba Prout BBa_K1846004_sequence 1 atggcagtaaagatttcaggcgtcctgaaagacggcacaggcaaaccagtacagaactgcaccattcagcttaaagcccgtcgtaacagcaccacggtggtggtgaacacggtgggctcagagaatccggatgaagccgggcgttacagcatggatgtggagtacggtcagtacagtgtcatccttcaggttgacggttttccaccatcccacgccgggaccatcaccgtgtatgaagattcacaaccggggacgctgaatgattttctctgtgccatgacggaggatgatgcccgtccggaggtgctgcgtcgtcttgaactgatggtggaagaagtggcgcgtaacgcgtccgtggtggcacagagtacggcagacgcgaagaaatcagcgggtgatgccagtgcatcagcggctcaggtcgcagcccttgtgactgatgcaactgactcagcacgcgccgcaagcacgtccgcaggccaggctgcatcgtcagctcaggaagcgtcctccggcgcagaagcggcatcagcaaaggccactgaagcggaaaaaagtgcggctgccgcagagtcctcaaaaaacgcagcggcgaccagtgccggtgcagcgaaaacgtcagaaaccaatgcggctgcgtcacaacaatcagccgccacgtctgcctccaccgcggccacgaaagcgtcagaggccgcaacttcagcacgtgatgcggtggcctcaaaagaggcagcaaaatcatcagaaacgaacgcatcatcaagtgccggtcgtgcagcttcctcggcaacggcggcagaaaattctgcccgtgcggccaaaacgtccgaaaccaatgcccgttcatctgaaaccgcagcggaacgtagcgcctctgccgcggcagacgcaaaaacagcagcggccgggagtgcgtcaacggcatccacgaaggcgacagaggctgcgggcagtgcggtatcagcatcccagagcaaaagtgcggcagaagcagcggcaattcgtgcaaaaaattcggcaaaacgtgcagaagatattgcttcagcggtcgcgcttgaggatgcggacacaacgcgtaaggggattgtgcagttgagcagtgcaaccaacagcacgtctgaaacgcttgctgcaacgccaaaggcggttaaggtggtaatggatgaaacgaaccgtaaagccccactggacagtccggcactgaccggcacgccaacagcaccaaccgcgctccgtggcacaaacaatacccagattgcgaacaccgcttttgtactggccgcgattgcagatgttatcgacgcgtcacctgacgcactgaatacgctgaatgaactggccgcagcgctcgggaatgatccagattttgctaccaccatgactaacgcgcttgcgggtaaacaaccgaagaacgcgacactgacggcgctggcagggctttccacggcgaaaaataaattaccgtattttgcggaaaatgatgccgccagcctgactgaactgactcaggttggccgtgatattctggcaaaaaattccgttgcagatgttcttgaataccttggggccggtgaaaactcggcctttccggctggtgcgccgatcccgtggccatcagatatcgttccgtctggctacgtcctgatgcaggggcaggcgtttgacaaatcagcctacccaaaacttgctgtcgcgtatccatcgggtgtgcttcctgatatgcgtggctggacaatcaaggggaaaccggccagcggtcgtgctgtattgtctcaggaacaggatggcattaagtcgcacacccacagtgccagtgcatccggtacggatttggggacgaaaaccacatcgtcgtttgattacgggacgaaaacaacaggcagtttcgattacggcaccaaatcgacgaataacacgggggctcatgctcacagtctgagcggttcaacaggggccgcgggtgctcatgcccacacaagtggtttacgtatgaacagttctggctggagtcagtatggcacagcaaccattacaggcagtttatccacagttaaaggcaccagcacacagggtattgcttatttatcgaaaacggacagtcagggcagccacagtcactcattgtccggtacagccgtgagtgccggtgcacatgcgcatacagttggtattggtgcgcaccagcatccggttgttatcggtgctcatgcccattctttcagtattggttcacacggccacaccatcaccgttaacgctgcgggtaacgcggaaaacaccgtcaaaaacattgcatttaactatattgtgcgtcttgcttaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z