##gff-version 3
##sequence-region BBa_K1846007 1 1696
BBa_K1846007	.	engineered_region	47	888	.	+	0	ID=BBa_P0140;Name=BBa_P0140
BBa_K1846007	.	ribosome_entry_site	47	60	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_P0140
BBa_K1846007	.	conserved_region	53	56	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K1846007	.	terminator	848	888	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0140
BBa_K1846007	.	polyA_site	875	888	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1846007	.	engineered_region	848	888	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1846007	.	stop_codon	881	881	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1846007	.	stem_loop	855	874	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1846007	.	terminator	760	839	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0140
BBa_K1846007	.	stem_loop	771	814	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1846007	.	engineered_region	760	839	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1846007	.	CDS	67	726	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0140
BBa_K1846007	.	CDS	70	686	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1846007	.	tag	687	720	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1846007	.	engineered_tag	727	751	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1846007	.	promoter	1	38	.	+	0	ID=BBa_I14033;Name=BBa_I14033
BBa_K1846007	.	promoter	27	32	.	+	0	ID=annotation1026173;Name=-10;Parent=BBa_I14033
BBa_K1846007	.	promoter	4	9	.	+	0	ID=annotation1026172;Name=-35;Parent=BBa_I14033
BBa_K1846007	.	operator	1	38	.	+	0	ID=annotation1026174;Name=P(Cat);Parent=BBa_I14033
BBa_K1846007	.	engineered_region	897	1696	.	+	0	ID=BBa_K1846001;Name=BBa_K1846001
BBa_K1846007	.	engineered_region	989	994	.	+	0	ID=BBa_S05278;Name=BBa_S05278;Parent=BBa_K1846001
BBa_K1846007	.	engineered_region	989	1000	.	+	0	ID=annotation2439242;Name=BBa_B0034;Parent=BBa_S05278
BBa_K1846007	.	engineered_region	1007	1591	.	+	0	ID=annotation2439243;Name=BBa_K1846002;Parent=BBa_S05278
BBa_K1846007	.	conserved_region	993	996	.	+	0	ID=annotation2439241;Name=conserved;Parent=BBa_S05278
BBa_K1846007	.	CDS	995	1579	.	+	0	ID=BBa_K1846002;Name=BBa_K1846002;Parent=BBa_K1846001
BBa_K1846007	.	promoter	897	950	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1846001
BBa_K1846007	.	non_covalent_binding_site	897	915	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1846007	.	promoter	916	921	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1846007	.	promoter	939	944	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1846007	.	non_covalent_binding_site	922	940	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1846007	.	engineered_region	897	950	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1846007	.	engineered_region	951	976	.	+	0	ID=BBa_S05276;Name=BBa_S05276;Parent=BBa_K1846001
BBa_K1846007	.	engineered_region	1013	1024	.	+	0	ID=annotation2439177;Name=BBa_B0034;Parent=BBa_S05276
BBa_K1846007	.	non_covalent_binding_site	976	994	.	+	0	ID=annotation2439174;Name=TetR 2;Parent=BBa_S05276
BBa_K1846007	.	engineered_region	951	1004	.	+	0	ID=annotation2439171;Name=BBa_R0040;Parent=BBa_S05276
BBa_K1846007	.	promoter	970	975	.	+	0	ID=annotation2439173;Name=-35;Parent=BBa_S05276
BBa_K1846007	.	conserved_region	1017	1020	.	+	0	ID=annotation2439176;Name=conserved;Parent=BBa_S05276
BBa_K1846007	.	promoter	993	998	.	+	0	ID=annotation2439175;Name=-10;Parent=BBa_S05276
BBa_K1846007	.	non_covalent_binding_site	951	969	.	+	0	ID=annotation2439172;Name=TetR 1;Parent=BBa_S05276
BBa_K1846007	.	ribosome_entry_site	977	988	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1846001
BBa_K1846007	.	conserved_region	981	984	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1846007	.	CDS	1580	1616	.	+	0	ID=BBa_K1846002;Name=BBa_K1846002;Parent=BBa_K1846001
BBa_K1846007	.	engineered_region	1580	1616	.	+	0	ID=BBa_S05279;Name=BBa_S05279;Parent=BBa_K1846001
BBa_K1846007	.	engineered_region	2173	2252	.	+	0	ID=annotation2439262;Name=BBa_B0010;Parent=BBa_S05279
BBa_K1846007	.	engineered_region	1580	2164	.	+	0	ID=annotation2439261;Name=BBa_K1846002;Parent=BBa_S05279
BBa_K1846007	.	stem_loop	2184	2227	.	+	0	ID=annotation2439263;Name=stem_loop;Parent=BBa_S05279
BBa_K1846007	.	terminator	1617	1696	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1846001
BBa_K1846007	.	stem_loop	1628	1671	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1846007	.	engineered_region	1617	1696	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1846007	.	promoter	951	976	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1846001
BBa_K1846007	.	non_covalent_binding_site	951	969	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1846007	.	promoter	970	975	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1846007	.	promoter	993	998	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1846007	.	non_covalent_binding_site	976	994	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1846007	.	engineered_region	951	1004	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1846007	.	ribosome_entry_site	989	994	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1846001
BBa_K1846007	.	conserved_region	993	996	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1846007
ggcacgtaagaggttccaactttcaccataatgaaacatactagagtcacacaggaaacctactagatgtccagattag
ataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgc
ccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccatt
gagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgcta
aaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagta
tgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgct
gtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctacta
ctgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcgg
ccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgct
ttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagc
acgatgtcaaagaatacaagcattgactaaagaggagaaaatacaaatggcgttccgtatgagtgaacaaccacgtacc
attaaaatttataatctgctggccggtactaatgaatttattggtgaaggtgacgcatatattccgcctcataccggcc
tgcctgcaaacagtaccgatattgcaccgccagatattccggctggctttgtggctgttttcaacagtgatgaggcatc
gtggcatctcgttgaagaccatcgtggtaaaaccgtctatgacgtggcttccggcgacgcgttatttatttctgaactc
ggtccgttaccggaaaattttacctggttatcgccgggtggggaatatcagaagtggaacggcacagcctgggtgaagg
atacggaagcagaaaaactgttccgtatccgtgaggcggaagaaacaaaaaaaagcctgatgcaagtagccagtgagca
tattgcgccgcttcaggatgcggcggatctggaaattgcaacgaaggaagaaacctcgttgctggaagcctggaagaag
tatcgtgtgttgctgaaccgtgttgatacatcaactgcacctgatattgagtggcctgctgtccctgttatggagtaat
aacgctgatactgatagtgctagtagtgtagatcgcccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctc