TetR+tfa+
type: ComponentDefinition
persistent identity: BBa K1846007
display id: BBa_K1846007
version: 1
was generated by: iGEM to SBOL conversion
description: Bacteriophage lambda tail fibre assembly (tfa) under TetR regulation
created: 2015-09-16T11:00:00Z
modified: 2015-09-20T01:47:20Z
mutable provenance: ...
owned by: james
owned by: myers
mutable description: A circuit controlling the production of the tail fibre assembly (tfa) protein of bacteriophage lambda. The tfa gene operates under a TetR repressible promoter, while a second circuit produces TetR (tetracycline repressor) under control of a P(Cat) promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2272_
m user id: 24377
owner id: 27305
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: ...
star: false
creator: Luba Prout
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I14033
component: BBa_K1846001
component: BBa_P0140
sequence annotation: BBa_I14033
sequence annotation: BBa_P0140
sequence annotation: BBa_K1846001
sequence: https://synbiohub.org/public/igem/BBa_K1846007_sequence/1

BBa_I14033
type: Component
persistent identity: component2465835
display id: component2465835
version: 1
top level: TetR+tfa+
definition: Cat
access: public

BBa_K1846001
type: Component
persistent identity: component2465868
display id: component2465868
version: 1
top level: TetR+tfa+
definition: tfa+
access: public

BBa_P0140
type: Component
persistent identity: component2465848
display id: component2465848
version: 1
top level: TetR+tfa+
definition: BBa_P0140
access: public

BBa_I14033
type: SequenceAnnotation
persistent identity: annotation2465835
display id: annotation2465835
version: 1
top level: TetR+tfa+
location: https://synbiohub.org/public/igem/BBa_K1846007/annotation2465835/range2465835/1
component: BBa_I14033

https://synbiohub.org/public/igem/BBa_K1846007/annotation2465835/range2465835/1
type: Range
persistent identity: range2465835
display id: range2465835
version: 1
top level: TetR+tfa+
direction: direction/reverse
start: 1
end: 38
orientation: inline

BBa_P0140
type: SequenceAnnotation
persistent identity: annotation2465848
display id: annotation2465848
version: 1
top level: TetR+tfa+
location: https://synbiohub.org/public/igem/BBa_K1846007/annotation2465848/range2465848/1
component: BBa_P0140

https://synbiohub.org/public/igem/BBa_K1846007/annotation2465848/range2465848/1
type: Range
persistent identity: range2465848
display id: range2465848
version: 1
top level: TetR+tfa+
direction: direction/reverse
start: 47
end: 888
orientation: inline

BBa_K1846001
type: SequenceAnnotation
persistent identity: annotation2465868
display id: annotation2465868
version: 1
top level: TetR+tfa+
location: https://synbiohub.org/public/igem/BBa_K1846007/annotation2465868/range2465868/1
component: BBa_K1846001

https://synbiohub.org/public/igem/BBa_K1846007/annotation2465868/range2465868/1
type: Range
persistent identity: range2465868
display id: range2465868
version: 1
top level: TetR+tfa+
direction: direction/reverse
start: 897
end: 1696
orientation: inline

Cat
type: ComponentDefinition
persistent identity: BBa I14033
display id: BBa_I14033
version: 1
was generated by: iGEM to SBOL conversion
description: P(Cat)
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
mutable provenance: Plasmid pACYC184
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive Promoter, Medium Transcription
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P(Cat)
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14033_sequence/1

P(Cat)
type: SequenceAnnotation
persistent identity: annotation1026174
display id: annotation1026174
version: 1
top level: Cat
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026174/range1026174/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_I14033/annotation1026174/range1026174/1
type: Range
persistent identity: range1026174
display id: range1026174
version: 1
top level: Cat
direction: direction/forward
start: 1
end: 38
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1026172
display id: annotation1026172
version: 1
top level: Cat
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026172/range1026172/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I14033/annotation1026172/range1026172/1
type: Range
persistent identity: range1026172
display id: range1026172
version: 1
top level: Cat
direction: direction/forward
start: 4
end: 9
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1026173
display id: annotation1026173
version: 1
top level: Cat
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026173/range1026173/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I14033/annotation1026173/range1026173/1
type: Range
persistent identity: range1026173
display id: range1026173
version: 1
top level: Cat
direction: direction/forward
start: 27
end: 32
orientation: inline

BBa_P0140
type: ComponentDefinition
persistent identity: BBa P0140
display id: BBa_P0140
version: 1
was generated by: iGEM to SBOL conversion
description: PoPS -> TetR [S0163]
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Protein generator converting TIPS to the protein TetR. Used as the input section for Quad Part Inverter Q01140.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_C0040
component: BBa_B0031
sequence annotation: BBa_B0031
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_P0140_sequence/1

BBa_B0012
type: Component
persistent identity: component944458
display id: component944458
version: 1
top level: BBa_P0140
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component944448
display id: component944448
version: 1
top level: BBa_P0140
definition: BBa_B0010
access: public

BBa_C0040
type: Component
persistent identity: component944442
display id: component944442
version: 1
top level: BBa_P0140
definition: tetR
access: public

BBa_B0031
type: Component
persistent identity: component944432
display id: component944432
version: 1
top level: BBa_P0140
definition: BBa_B0031
access: public

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944432
display id: annotation944432
version: 1
top level: BBa_P0140
location: https://synbiohub.org/public/igem/BBa_P0140/annotation944432/range944432/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0140/annotation944432/range944432/1
type: Range
persistent identity: range944432
display id: range944432
version: 1
top level: BBa_P0140
direction: direction/forward
start: 1
end: 14
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation944442
display id: annotation944442
version: 1
top level: BBa_P0140
location: https://synbiohub.org/public/igem/BBa_P0140/annotation944442/range944442/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_P0140/annotation944442/range944442/1
type: Range
persistent identity: range944442
display id: range944442
version: 1
top level: BBa_P0140
direction: direction/forward
start: 21
end: 680
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944448
display id: annotation944448
version: 1
top level: BBa_P0140
location: https://synbiohub.org/public/igem/BBa_P0140/annotation944448/range944448/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0140/annotation944448/range944448/1
type: Range
persistent identity: range944448
display id: range944448
version: 1
top level: BBa_P0140
direction: direction/forward
start: 714
end: 793
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944458
display id: annotation944458
version: 1
top level: BBa_P0140
location: https://synbiohub.org/public/igem/BBa_P0140/annotation944458/range944458/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0140/annotation944458/range944458/1
type: Range
persistent identity: range944458
display id: range944458
version: 1
top level: BBa_P0140
direction: direction/forward
start: 802
end: 842
orientation: inline

tfa+
type: ComponentDefinition
persistent identity: BBa K1846001
display id: BBa_K1846001
version: 1
was generated by: iGEM to SBOL conversion
description: Bacteriophage lambda tail fibre assembly protein circuit
created: 2015-08-25T11:00:00Z
modified: 2015-09-20T01:26:15Z
mutable provenance: ...
owned by: james
owned by: myers
mutable description: The tfa (tail fibre assembly) protein of bacteriophage Lambda assists in the assembly of the stf (short tail fibre) protein into a functional tail fibre. This part provides the gene sequence for tfa, together with a TetR repressible promoter (TetO, available separately as BioBrick part BBa_R0040), ribosome binding site (available separately as part BBa_B0034) and an rrNB T1 terminator (available separately as BioBrick part BBa_B0010).
discontinued: false
dominant: false
experience: experience/None
group u list: _2272_
m user id: 24377
owner id: 27305
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: ...
star: false
creator: Luba Prout
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_B0010
component: BBa_S05276
component: BBa_B0034
component: BBa_K1846002
component: BBa_S05278
component: BBa_S05279
sequence annotation: BBa_R0040
sequence annotation: BBa_S05276
sequence annotation: BBa_B0034
sequence annotation: BBa_S05278
sequence annotation: BBa_K1846002
sequence annotation: BBa_S05279
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K1846001_sequence/1

BBa_R0040
type: Component
persistent identity: component2439264
display id: component2439264
version: 1
top level: tfa+
definition: p(tetR)
access: public

BBa_B0010
type: Component
persistent identity: component2439281
display id: component2439281
version: 1
top level: tfa+
definition: BBa_B0010
access: public

BBa_S05276
type: Component
persistent identity: component2439272
display id: component2439272
version: 1
top level: tfa+
definition: BBa_S05276
access: public

BBa_R0040
type: Component
persistent identity: component2439269
display id: component2439269
version: 1
top level: tfa+
definition: p(tetR)
access: public

BBa_B0034
type: Component
persistent identity: component2439275
display id: component2439275
version: 1
top level: tfa+
definition: BBa_B0034
access: public

BBa_B0034
type: Component
persistent identity: component2439274
display id: component2439274
version: 1
top level: tfa+
definition: BBa_B0034
access: public

BBa_K1846002
type: Component
persistent identity: component2439278
display id: component2439278
version: 1
top level: tfa+
definition: tfa
access: public

BBa_S05278
type: Component
persistent identity: component2439277
display id: component2439277
version: 1
top level: tfa+
definition: BBa_S05278
access: public

BBa_S05279
type: Component
persistent identity: component2439280
display id: component2439280
version: 1
top level: tfa+
definition: BBa_S05279
access: public

BBa_K1846002
type: Component
persistent identity: component2439279
display id: component2439279
version: 1
top level: tfa+
definition: tfa
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2439264
display id: annotation2439264
version: 1
top level: tfa+
location: https://synbiohub.org/public/igem/BBa_K1846001/annotation2439264/range2439264/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1846001/annotation2439264/range2439264/1
type: Range
persistent identity: range2439264
display id: range2439264
version: 1
top level: tfa+
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2439269
display id: annotation2439269
version: 1
top level: tfa+
location: https://synbiohub.org/public/igem/BBa_K1846001/annotation2439269/range2439269/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1846001/annotation2439269/range2439269/1
type: Range
persistent identity: range2439269
display id: range2439269
version: 1
top level: tfa+
direction: direction/forward
start: 55
end: 80
orientation: inline

BBa_S05276
type: SequenceAnnotation
persistent identity: annotation2439272
display id: annotation2439272
version: 1
top level: tfa+
location: https://synbiohub.org/public/igem/BBa_K1846001/annotation2439272/range2439272/1
component: BBa_S05276

https://synbiohub.org/public/igem/BBa_K1846001/annotation2439272/range2439272/1
type: Range
persistent identity: range2439272
display id: range2439272
version: 1
top level: tfa+
direction: direction/reverse
start: 55
end: 80
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2439274
display id: annotation2439274
version: 1
top level: tfa+
location: https://synbiohub.org/public/igem/BBa_K1846001/annotation2439274/range2439274/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1846001/annotation2439274/range2439274/1
type: Range
persistent identity: range2439274
display id: range2439274
version: 1
top level: tfa+
direction: direction/reverse
start: 81
end: 92
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2439275
display id: annotation2439275
version: 1
top level: tfa+
location: https://synbiohub.org/public/igem/BBa_K1846001/annotation2439275/range2439275/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1846001/annotation2439275/range2439275/1
type: Range
persistent identity: range2439275
display id: range2439275
version: 1
top level: tfa+
direction: direction/reverse
start: 93
end: 98
orientation: inline

BBa_S05278
type: SequenceAnnotation
persistent identity: annotation2439277
display id: annotation2439277
version: 1
top level: tfa+
location: https://synbiohub.org/public/igem/BBa_K1846001/annotation2439277/range2439277/1
component: BBa_S05278

https://synbiohub.org/public/igem/BBa_K1846001/annotation2439277/range2439277/1
type: Range
persistent identity: range2439277
display id: range2439277
version: 1
top level: tfa+
direction: direction/reverse
start: 93
end: 98
orientation: inline

BBa_K1846002
type: SequenceAnnotation
persistent identity: annotation2439278
display id: annotation2439278
version: 1
top level: tfa+
location: https://synbiohub.org/public/igem/BBa_K1846001/annotation2439278/range2439278/1
component: BBa_K1846002

https://synbiohub.org/public/igem/BBa_K1846001/annotation2439278/range2439278/1
type: Range
persistent identity: range2439278
display id: range2439278
version: 1
top level: tfa+
direction: direction/reverse
start: 99
end: 683
orientation: inline

BBa_S05279
type: SequenceAnnotation
persistent identity: annotation2439280
display id: annotation2439280
version: 1
top level: tfa+
location: https://synbiohub.org/public/igem/BBa_K1846001/annotation2439280/range2439280/1
component: BBa_S05279

https://synbiohub.org/public/igem/BBa_K1846001/annotation2439280/range2439280/1
type: Range
persistent identity: range2439280
display id: range2439280
version: 1
top level: tfa+
direction: direction/reverse
start: 684
end: 720
orientation: inline

BBa_K1846002
type: SequenceAnnotation
persistent identity: annotation2439279
display id: annotation2439279
version: 1
top level: tfa+
location: https://synbiohub.org/public/igem/BBa_K1846001/annotation2439279/range2439279/1
component: BBa_K1846002

https://synbiohub.org/public/igem/BBa_K1846001/annotation2439279/range2439279/1
type: Range
persistent identity: range2439279
display id: range2439279
version: 1
top level: tfa+
direction: direction/reverse
start: 684
end: 720
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2439281
display id: annotation2439281
version: 1
top level: tfa+
location: https://synbiohub.org/public/igem/BBa_K1846001/annotation2439281/range2439281/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1846001/annotation2439281/range2439281/1
type: Range
persistent identity: range2439281
display id: range2439281
version: 1
top level: tfa+
direction: direction/forward
start: 721
end: 800
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.2 (weak) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
top level: BBa_B0031
direction: direction/forward
start: 7
end: 10
orientation: inline

tfa
type: ComponentDefinition
persistent identity: BBa K1846002
display id: BBa_K1846002
version: 1
was generated by: iGEM to SBOL conversion
description: Bacteriophage lambda tail fibre assembly (tfa) protein
created: 2015-08-25T11:00:00Z
modified: 2015-09-20T01:20:28Z
mutable provenance: The sequence has been adapted from the genomic sequence of bacteriophage Lambda.
owned by: james
owned by: myers
mutable description: The tfa (tail fibre assembly) protein of bacteriophage Lambda assists in the assembly of the stf (short tail fibre) protein into a functional tail fibre.
discontinued: false
dominant: false
experience: experience/None
group u list: _2272_
m user id: 24377
owner id: 27305
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Sequence was optimised to remove illegal restriction sites.
star: false
creator: Luba Prout
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1846002_sequence/1

BBa_S05276
type: ComponentDefinition
persistent identity: BBa S05276
display id: BBa_S05276
version: 1
was generated by: iGEM to SBOL conversion
description: R0040:B0034
created: 2015-08-27T11:00:00Z
modified: 2015-08-28T03:23:28Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 27305
owner id: 27305
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Barbara Steijl
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
sequence annotation: TetR 1
sequence annotation: BBa_R0040
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_S05276_sequence/1

TetR 1
type: SequenceAnnotation
persistent identity: annotation2439172
display id: annotation2439172
version: 1
top level: BBa_S05276
location: https://synbiohub.org/public/igem/BBa_S05276/annotation2439172/range2439172/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_S05276/annotation2439172/range2439172/1
type: Range
persistent identity: range2439172
display id: range2439172
version: 1
top level: BBa_S05276
direction: direction/forward
start: 1
end: 19
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2439171
display id: annotation2439171
version: 1
top level: BBa_S05276
location: https://synbiohub.org/public/igem/BBa_S05276/annotation2439171/range2439171/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_S05276/annotation2439171/range2439171/1
type: Range
persistent identity: range2439171
display id: range2439171
version: 1
top level: BBa_S05276
direction: direction/forward
start: 1
end: 54
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2439173
display id: annotation2439173
version: 1
top level: BBa_S05276
location: https://synbiohub.org/public/igem/BBa_S05276/annotation2439173/range2439173/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_S05276/annotation2439173/range2439173/1
type: Range
persistent identity: range2439173
display id: range2439173
version: 1
top level: BBa_S05276
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation2439174
display id: annotation2439174
version: 1
top level: BBa_S05276
location: https://synbiohub.org/public/igem/BBa_S05276/annotation2439174/range2439174/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_S05276/annotation2439174/range2439174/1
type: Range
persistent identity: range2439174
display id: range2439174
version: 1
top level: BBa_S05276
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2439175
display id: annotation2439175
version: 1
top level: BBa_S05276
location: https://synbiohub.org/public/igem/BBa_S05276/annotation2439175/range2439175/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_S05276/annotation2439175/range2439175/1
type: Range
persistent identity: range2439175
display id: range2439175
version: 1
top level: BBa_S05276
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2439177
display id: annotation2439177
version: 1
top level: BBa_S05276
location: https://synbiohub.org/public/igem/BBa_S05276/annotation2439177/range2439177/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_S05276/annotation2439177/range2439177/1
type: Range
persistent identity: range2439177
display id: range2439177
version: 1
top level: BBa_S05276
direction: direction/reverse
start: 63
end: 74
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2439176
display id: annotation2439176
version: 1
top level: BBa_S05276
location: https://synbiohub.org/public/igem/BBa_S05276/annotation2439176/range2439176/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_S05276/annotation2439176/range2439176/1
type: Range
persistent identity: range2439176
display id: range2439176
version: 1
top level: BBa_S05276
direction: direction/forward
start: 67
end: 70
orientation: inline

BBa_S05278
type: ComponentDefinition
persistent identity: BBa S05278
display id: BBa_S05278
version: 1
was generated by: iGEM to SBOL conversion
description: B0034:K1846002
created: 2015-08-27T11:00:00Z
modified: 2015-08-28T03:51:09Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 27305
owner id: 27305
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Barbara Steijl
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: BBa_K1846002
sequence: https://synbiohub.org/public/igem/BBa_S05278_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2439242
display id: annotation2439242
version: 1
top level: BBa_S05278
location: https://synbiohub.org/public/igem/BBa_S05278/annotation2439242/range2439242/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_S05278/annotation2439242/range2439242/1
type: Range
persistent identity: range2439242
display id: range2439242
version: 1
top level: BBa_S05278
direction: direction/reverse
start: 1
end: 12
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2439241
display id: annotation2439241
version: 1
top level: BBa_S05278
location: https://synbiohub.org/public/igem/BBa_S05278/annotation2439241/range2439241/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_S05278/annotation2439241/range2439241/1
type: Range
persistent identity: range2439241
display id: range2439241
version: 1
top level: BBa_S05278
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K1846002
type: SequenceAnnotation
persistent identity: annotation2439243
display id: annotation2439243
version: 1
top level: BBa_S05278
location: https://synbiohub.org/public/igem/BBa_S05278/annotation2439243/range2439243/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_S05278/annotation2439243/range2439243/1
type: Range
persistent identity: range2439243
display id: range2439243
version: 1
top level: BBa_S05278
direction: direction/reverse
start: 19
end: 603
orientation: inline

BBa_S05279
type: ComponentDefinition
persistent identity: BBa S05279
display id: BBa_S05279
version: 1
was generated by: iGEM to SBOL conversion
description: K1846002:B0010
created: 2015-08-27T11:00:00Z
modified: 2015-08-28T03:52:59Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 27305
owner id: 27305
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Barbara Steijl
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
sequence annotation: BBa_K1846002
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_S05279_sequence/1

BBa_K1846002
type: SequenceAnnotation
persistent identity: annotation2439261
display id: annotation2439261
version: 1
top level: BBa_S05279
location: https://synbiohub.org/public/igem/BBa_S05279/annotation2439261/range2439261/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_S05279/annotation2439261/range2439261/1
type: Range
persistent identity: range2439261
display id: range2439261
version: 1
top level: BBa_S05279
direction: direction/reverse
start: 1
end: 585
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2439262
display id: annotation2439262
version: 1
top level: BBa_S05279
location: https://synbiohub.org/public/igem/BBa_S05279/annotation2439262/range2439262/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_S05279/annotation2439262/range2439262/1
type: Range
persistent identity: range2439262
display id: range2439262
version: 1
top level: BBa_S05279
direction: direction/forward
start: 594
end: 673
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2439263
display id: annotation2439263
version: 1
top level: BBa_S05279
location: https://synbiohub.org/public/igem/BBa_S05279/annotation2439263/range2439263/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_S05279/annotation2439263/range2439263/1
type: Range
persistent identity: range2439263
display id: range2439263
version: 1
top level: BBa_S05279
direction: direction/forward
start: 605
end: 648
orientation: inline

https://synbiohub.org/public/igem/BBa_K1846007_sequence/1
type: Sequence
persistent identity: BBa K1846007 sequence
display id: BBa_K1846007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: ggcacgtaagaggttccaactttcaccataatgaaacatactagagtcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgatgtcaaagaatacaagcattgactaaagaggagaaaatacaaatggcgttccgtatgagtgaacaaccacgtaccattaaaatttataatctgctggccggtactaatgaatttattggtgaaggtgacgcatatattccgcctcataccggcctgcctgcaaacagtaccgatattgcaccgccagatattccggctggctttgtggctgttttcaacagtgatgaggcatcgtggcatctcgttgaagaccatcgtggtaaaaccgtctatgacgtggcttccggcgacgcgttatttatttctgaactcggtccgttaccggaaaattttacctggttatcgccgggtggggaatatcagaagtggaacggcacagcctgggtgaaggatacggaagcagaaaaactgttccgtatccgtgaggcggaagaaacaaaaaaaagcctgatgcaagtagccagtgagcatattgcgccgcttcaggatgcggcggatctggaaattgcaacgaaggaagaaacctcgttgctggaagcctggaagaagtatcgtgtgttgctgaaccgtgttgatacatcaactgcacctgatattgagtggcctgctgtccctgttatggagtaataacgctgatactgatagtgctagtagtgtagatcgcccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14033_sequence/1
type: Sequence
persistent identity: BBa I14033 sequence
display id: BBa_I14033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcacgtaagaggttccaactttcaccataatgaaaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P0140_sequence/1
type: Sequence
persistent identity: BBa P0140 sequence
display id: BBa_P0140_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1846001_sequence/1
type: Sequence
persistent identity: BBa K1846001 sequence
display id: BBa_K1846001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgatgtcaaagaatacaagcattgactaaagaggagaaaatacaaatggcgttccgtatgagtgaacaaccacgtaccattaaaatttataatctgctggccggtactaatgaatttattggtgaaggtgacgcatatattccgcctcataccggcctgcctgcaaacagtaccgatattgcaccgccagatattccggctggctttgtggctgttttcaacagtgatgaggcatcgtggcatctcgttgaagaccatcgtggtaaaaccgtctatgacgtggcttccggcgacgcgttatttatttctgaactcggtccgttaccggaaaattttacctggttatcgccgggtggggaatatcagaagtggaacggcacagcctgggtgaaggatacggaagcagaaaaactgttccgtatccgtgaggcggaagaaacaaaaaaaagcctgatgcaagtagccagtgagcatattgcgccgcttcaggatgcggcggatctggaaattgcaacgaaggaagaaacctcgttgctggaagcctggaagaagtatcgtgtgttgctgaaccgtgttgatacatcaactgcacctgatattgagtggcctgctgtccctgttatggagtaataacgctgatactgatagtgctagtagtgtagatcgcccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0031_sequence/1
type: Sequence
persistent identity: BBa B0031 sequence
display id: BBa_B0031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1846002_sequence/1
type: Sequence
persistent identity: BBa K1846002 sequence
display id: BBa_K1846002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcgttccgtatgagtgaacaaccacgtaccattaaaatttataatctgctggccggtactaatgaatttattggtgaaggtgacgcatatattccgcctcataccggcctgcctgcaaacagtaccgatattgcaccgccagatattccggctggctttgtggctgttttcaacagtgatgaggcatcgtggcatctcgttgaagaccatcgtggtaaaaccgtctatgacgtggcttccggcgacgcgttatttatttctgaactcggtccgttaccggaaaattttacctggttatcgccgggtggggaatatcagaagtggaacggcacagcctgggtgaaggatacggaagcagaaaaactgttccgtatccgtgaggcggaagaaacaaaaaaaagcctgatgcaagtagccagtgagcatattgcgccgcttcaggatgcggcggatctggaaattgcaacgaaggaagaaacctcgttgctggaagcctggaagaagtatcgtgtgttgctgaaccgtgttgatacatcaactgcacctgatattgagtggcctgctgtccctgttatggagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05276_sequence/1
type: Sequence
persistent identity: BBa S05276 sequence
display id: BBa_S05276_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatgtcaaagaatacaagcattgact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05278_sequence/1
type: Sequence
persistent identity: BBa S05278 sequence
display id: BBa_S05278_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atacaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05279_sequence/1
type: Sequence
persistent identity: BBa S05279 sequence
display id: BBa_S05279_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacgctgatactgatagtgctagtagtgtagatcgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z