BBa_K1848004
1
BBa_K1848004
Human Gut Hormone Glp1 (7-37) with upstream signal peptide PhoA
2015-09-17T11:00:00Z
2015-09-18T03:30:49Z
PhoA comes from E. coli strain K12.
Same as part BBa-K1848001 except possessing upstream signal peptide PhoA. This signal peptide shuttle's the target protein into the extracellular space via the type II secretion pathway. This signal peptide is E. coli specific and might not work well within other species of gram negative bacteria.
false
false
_2275_
27274
27274
9
false
Notably, concentration of secreted protein is much lower than internal concentration. As such supernatant need be concentrated before protein screening occurs.
false
Samuel Magaziner
BBa_K1848004_sequence
1
atgaaacaatctaccattgcactggcactgctgccgctgctgttcaccccggtcactaaagcgcacgcggaaggcacttttacctctgacgtttcctcttatctggaaggccaggctgctaaagagttcatcgcgtggctggttaaaggccgtggttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z