BBa_K1848004 1 BBa_K1848004 Human Gut Hormone Glp1 (7-37) with upstream signal peptide PhoA 2015-09-17T11:00:00Z 2015-09-18T03:30:49Z PhoA comes from E. coli strain K12. Same as part BBa-K1848001 except possessing upstream signal peptide PhoA. This signal peptide shuttle's the target protein into the extracellular space via the type II secretion pathway. This signal peptide is E. coli specific and might not work well within other species of gram negative bacteria. false false _2275_ 27274 27274 9 false Notably, concentration of secreted protein is much lower than internal concentration. As such supernatant need be concentrated before protein screening occurs. false Samuel Magaziner BBa_K1848004_sequence 1 atgaaacaatctaccattgcactggcactgctgccgctgctgttcaccccggtcactaaagcgcacgcggaaggcacttttacctctgacgtttcctcttatctggaaggccaggctgctaaagagttcatcgcgtggctggttaaaggccgtggttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z