LOCUS       BBa_K1848006            2681 bp    DNA     linear       08-May-2024
DEFINITION  LuxR/AHL driven Lysis Cassette with Protein level Timer Delay
ACCESSION   BBa_K1848006
VERSION     BBa_K1848006.1  
FEATURES             Location/Qualifiers
     promoter        1..6
                     /label=-35
     promoter        25..30
                     /label=-10
     misc_feature    6..25
                     /label=Lux Box
     promoter        1..30
                     /label=BBa_R0061
     misc_feature    43..46
                     /label=conserved
     RBS             39..50
                     /label=BBa_B0034
     CDS             60..767
                     /label=cI lambda
     misc_feature    768..800
                     /label=LVA
     misc_feature    807..831
                     /label=Help:Barcodes
     CDS             57..831
                     /label=BBa_C0051
     promoter        877..882
                     /label=-10
     promoter        854..859
                     /label=-35
     misc_feature    864..880
                     /label=OR1
     misc_feature    840..856
                     /label=OR2
     misc_feature    840..888
                     /label=BBa_R0051
     promoter        840..888
                     /label=BBa_R0051
     CDS             913..1569
                     /label=T4 holin
     CDS             897..1572
                     /label=BBa_K112805
     CDS             1597..2091
                     /label=T4 Endolysin
     CDS             1581..2094
                     /label=BBa_K112806
     stem_loop       2114..2157
                     /label=stem_loop
     misc_feature    2103..2182
                     /label=BBa_B0010
     terminator      2103..2182
                     /label=BBa_B0010
     stem_loop       2198..2217
                     /label=T7 TE
     misc_feature    2224..2224
                     /label=stop
     polyA_site      2218..2231
                     /label=polya
     misc_feature    2191..2231
                     /label=BBa_B0012
     terminator      2191..2231
                     /label=BBa_B0012
     promoter        2240..2274
                     /label=BBa_J23116
     CDS             2297..2590
                     /label=T4 antiholin
     CDS             2283..2593
                     /label=BBa_K112807
     stem_loop       2613..2656
                     /label=stem_loop
     misc_feature    2602..2681
                     /label=BBa_B0010
     terminator      2602..2681
                     /label=BBa_B0010
     misc_feature    897..2681
                     /label=BBa_K112808
ORIGIN
        1 ttgacacctg taggatcgta caggtataat tactagagaa agaggagaaa tactagatga
       61 gcacaaaaaa gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa
      121 tttatgaaaa aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg
      181 ggatggggca gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata
      241 acgccgcatt gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg
      301 ccagagaaat ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt
      361 atgagtaccc tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct
      421 ttaccaaagg tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat
      481 tctggcttga ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc
      541 ctgacggaat gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca
      601 tagccagact tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg
      661 tgtttttaca accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg
      721 ttgtggggaa agttatcgct agtcagtggc ctgaagagac gtttggcgct gcaaacgacg
      781 aaaactacgc tttagtagct taataacgct gatagtgcta gtgtagatcg ctactagagt
      841 aacaccgtgc gtgttgacta ttttacctct ggcggtgata atggttgcta ctagagctta
      901 aaaggagggt ctatggcagc acctagaata tcattttcgc cctctgatat tctatttggt
      961 gttctcgatc gcttgttcaa agataacgct accgggaagg ttcttgcttc ccgggtagct
     1021 gtcgtaattc ttttgtttat aatggcgatt gtttggtata ggggagatag tttctttgag
     1081 tactataagc aatcaaagta tgaaacatac agtgaaatta ttgaaaagga aagaactgca
     1141 cgctttgaat ctgtcgccct ggaacaactc cagatagttc atatatcatc tgaggcagac
     1201 tttagtgcgg tgtattcttt ccgccctaaa aacttaaact attttgttga tattatagca
     1261 tacgaaggaa aattaccttc aacaataagt gaaaaatcac ttggaggata tcctgttgat
     1321 aaaactatgg atgaatatac agttcattta aatggacgtc attattattc caactcaaaa
     1381 tttgcttttt taccaactaa aaagcctact cccgaaataa actacatgta cagttgtcca
     1441 tattttaatt tggataatat ctatgctgga acgataacca tgtactggta tagaaatgat
     1501 catataagta atgaccgcct tgaatcaata tgtgctcagg cggccagaat attaggaagg
     1561 gctaaataat tatactagag atacttagga ggtattatga atatatttga aatgttacgt
     1621 atagatgaag gtcttagact taaaatctat aaagacacag aaggctatta cactattggc
     1681 atcggtcatt tgcttacaaa aagtccatca cttaatgctg ctaaatctga attagataaa
     1741 gctattgggc gtaattgcaa tggtgtaatt acaaaagatg aggctgaaaa actctttaat
     1801 caggatgttg atgctgctgt tcgcggaatc ctgagaaatg ctaaattaaa accggtttat
     1861 gattctcttg atgcggttcg tcgctgtgca ttgattaata tggttttcca aatgggagaa
     1921 accggtgtgg caggatttac taactcttta cgtatgcttc aacaaaaacg ctgggatgaa
     1981 gcagcagtta acttagctaa aagtagatgg tataatcaaa cacctaatcg cgcaaaacga
     2041 gtcattacaa cgtttagaac tggcacttgg gacgcgtata aaaatctata aagctactag
     2101 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     2161 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     2221 ctgcgtttat atactagagt tgacagctag ctcagtccta gggactatgc tagctactag
     2281 aggataggag gcctttatgg ccttaaaagc aacagcactt tttgccatgc taggattgtc
     2341 atttgtttta tctccatcga ttgaagcgaa tgtcgatcct cattttgata aatttatgga
     2401 atctggtatt aggcacgttt atatgctttt tgaaaataaa agcgtagaat cgtctgaaca
     2461 attctatagt tttatgagaa cgacctataa aaatgacccg tgctcttctg attttgaatg
     2521 tatagagcga ggcgcggaga tggcacaatc atacgctaga attatgaaca ttaaattgga
     2581 gactgaatga aattactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     2641 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct c
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