BBa_K1850003 1 BBa_K1850003 pRha - fimH - SpyTag_225 - HisTag_258 2015-09-14T11:00:00Z 2015-09-15T07:34:47Z fish comes from E. coli K-12 genome Titratable Rhamnose promtoter with fimH gene and HisTag insertion at position 258 for nickel binding. For production of modified type 1 pili. false false _2277_ 26421 26421 9 false edited out illegal cut sites false Lydia Goldberg annotation2456872 1 SpyTag range2456872 1 1005 1043 annotation2458008 1 B0034 range2458008 1 252 263 annotation2456859 1 HisTag range2456859 1 1143 1160 annotation2456855 1 pRha range2456855 1 4 243 annotation2456857 1 fimH range2456857 1 270 1229 BBa_K1850003_sequence 1 agacggcgaaatagtaatcacgaggtcaggttcttaccttaaattttcgacggaaaaccacgtaaaaaacgtcgatttttcaagatacagcgtgaattttcaggaaatgcggtgagcatcacatcaccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgagttcaggcgctttttagactggtcgtatactagagaaagaggagaaatactagatgaaacgagttattaccctgtttgctgtactgctgatgggctggtcggtaaatgcctggtcattcgcctgtaaaaccgccaatggtaccgctatccctattggcggtggcagcgccaatgtttatgtaaaccttgcgcccgtcgtgaatgtggggcaaaacctggtcgtggatctttcgacgcaaatcttttgccataacgattatccggaaaccattacagactatgtcacactgcaacgaggctcggcttatggcggcgtgttatctaatttttccgggaccgtaaaatatagtggcagtagctatccatttcctaccaccagcgaaacgccgcgcgttgtttataattcgagaacggataagccgtggccggtggcgctttatttgacgcctgtgagcagtgcgggcggggtggcgattaaagctggctcattaattgccgtgcttattttgcgacagaccaacaactataacagcgatgatttccagtttgtgtggaatatttacgccaataatgatgtggtggtgcctactggcggctgcgatgtttctgctcgtgatgtcaccgttactctgccggactaccctggttcagtgccaattcctcttaccgtttattgtgcgaaaagccaaaacctggggtattacctctccggcacaaccgcagatgcgggcaactcgattttcaccaataccgcgtcgttttcacctgcacagggcgtcggcgtacaggctcatattgtcatggttgatgcttacaagccaactaagttgacgcgcaacggtacgattattccagcgaataacacggtatcgttaggagcagtagggacttcggcggtgagtctgggattaacggcaaattatgcacatcatcatcaccaccaccgtaccggagggcaggtgactgcggggaatgtgcaatcgattattggcgtgacttttgtttatcaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z