##gff-version 3
##sequence-region BBa_K185003 1 2827
BBa_K185003	.	terminator	2787	2827	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K185003	.	polyA_site	2814	2827	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K185003	.	stem_loop	2794	2813	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K185003	.	stop_codon	2820	2820	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K185003	.	engineered_region	2787	2827	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K185003	.	terminator	2699	2778	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K185003	.	stem_loop	2710	2753	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K185003	.	engineered_region	2699	2778	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K185003	.	ribosome_entry_site	315	329	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K185003	.	engineered_region	315	329	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K185003	.	ribosome_entry_site	322	326	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K185003	.	ribosome_entry_site	315	329	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K185003	.	CDS	1	306	.	+	0	ID=BBa_K185047;Name=BBa_K185047
BBa_K185003	.	sequence_alteration	305	306	.	+	0	ID=annotation2047034;Name=mutation;Parent=BBa_K185047
BBa_K185003	.	tag	286	303	.	+	0	ID=annotation2047133;Name=His tag;Parent=BBa_K185047
BBa_K185003	.	start_codon	1	3	.	+	0	ID=annotation2046533;Name=start;Parent=BBa_K185047
BBa_K185003	.	CDS	4	285	.	+	0	ID=annotation2046560;Name=cds;Parent=BBa_K185047
BBa_K185003	.	stop_codon	306	306	.	+	0	ID=annotation2046574;Name=stop;Parent=BBa_K185047
BBa_K185003	.	CDS	336	2690	.	+	0	ID=BBa_K185049;Name=BBa_K185049
BBa_K185003	.	start_codon	336	338	.	+	0	ID=annotation2047316;Name=start;Parent=BBa_K185049
BBa_K185003	.	CDS	336	2690	.	+	0	ID=annotation2047318;Name=cds;Parent=BBa_K185049
BBa_K185003	.	stop_codon	2688	2690	.	+	0	ID=annotation2047317;Name=2353;Parent=BBa_K185049
>BBa_K185003
atggcgtattttctggattttgacgagcgggcactaaaggaatggcgaaagctgggctcgacggtacgtgaacagttga
aaaagaagctggttgaagtacttgagtcaccccggattgaagcaaacaagctccgtggtatgcctgattgttacaagat
taagctccggtcttcaggctatcgccttgtataccaggttatagacgagaaagttgtcgttttcgtgatttctgttggg
aaaagagaacgctcggaagtatatagcgaggcggtcaaacgcattctccatcatcatcatcatcactaatactagagat
taaagaggagaaatactagatgaatcctgagcgttctgaacgcattgaaatccccgtattgccgctgcgcgatgtggtg
gtttatccgcacatggtcatccccttatttgtcgggcgggaaaaatctatccgttgtctggaagcggcgatggaccatg
ataaaaaaattatgctggtcgcgcagaaagaagcttcaacggatgagccgggtgtaaacgatcttttcaccgtcgggac
cgtggcctctatattgcagatgctgaaactgcctgacggcaccgtcaaagtgctggtcgaggggttacagcgcgcgcgt
atttctgcgctctctgacaatggcgaacacttttctgcgaaggcggagtatctggagtcgccgaccattgatgagcggg
aacaggaagtgctggtgcgtactgcaatcagccagttcgaaggctacatcaagctgaacaaaaaaatcccaccagaagt
gctgacgtcgctgaatagcatcgacgatccggcgcgtctggcggataccattgctgcacatatgccgctgaaactggct
gacaaacagtctgttctggagatgtccgacgttaacgaacgtctggaatatctgatggcaatgatggaatcggaaatcg
atctgctgcaggttgagaaacgcattcgcaaccgcgttaaaaagcagatggagaaatcccagcgtgagtactatctgaa
cgagcaaatgaaagctattcagaaagaactcggtgaaatggacgacgcgccggacgaaaacgaagccctgaagcgcaaa
atcgacgcggcgaagatgccgaaagaggcaaaagagaaagcggaagcagagttgcagaagctgaaaatgatgtctccga
tgtcggcagaagcgaccgtagtgcgtggttatatcgactggatggtacaggtgccgtggaatgcgcgtagcaaggtcaa
aaaagacctgcgtcaggcgcaggaaatccttgataccgaccattatggtctggagcgcgtgaaagatcgaatccttgag
tatcttgcggttcaaagccgtgtcaacaaaatcaagggaccgatcctctgcctggtagggccgccgggggtaggtaaaa
cctctcttggtcagtccattgccaaagccaccgggcgtaaatatgtccgtatggcgctgggcggcgtgcgtgatgaagc
ggaaatccgtggtcaccgccgtacttacatcggttctatgccgggtaaactgatccagaaaatggcgaaagtgggcgtg
aaaaacccgctgttcctgctcgatgagatcgacaaaatgtcttctgacatgcgtggcgatccggcctctgcactgcttg
aagtgctggatccagagcagaacgtagcgttcagcgaccactacctggaagtggattacgatctcagcgacgtgatgtt
tgtcgcgacgtcgaactccatgaacattccggcaccgctgctggatcgtatggaagtgattcgcctctccggttatacc
gaagatgaaaaactgaacatcgccaaacgtcacctgctgccgaagcagattgaacgtaatgcactgaaaaaaggtgagc
tgaccgtcgacgatagcgccattatcggcattattcgttactacacccgtgaggcgggcgtgcgtggtctggagcgtga
aatctccaaactgtgtcgcaaagcggttaagcagttactgctcgataagtcattaaaacatatcgaaattaacggcgat
aacctgcatgactatctcggtgttcagcgtttcgactatggtcgcgcggataacgaaaaccgtgtcggtcaggtaaccg
gtctggcgtggacggaagtgggcggtgacttgctgaccattgaaaccgcatgtgttccgggtaaaggcaaactgaccta
taccggttcgctcggcgaagtgatgcaggagtccattcaggcggcgttaacggtggttcgtgcgcgtgcggaaaaactg
gggatcaaccctgatttttacgaaaaacgtgacatccacgtccacgtaccggaaggtgcgacgccgaaagatggtccga
gtgccggtattgctatgtgcaccgcgctggtttcttgcctgaccggtaacccggttcgtgccgatgtggcaatgaccgg
tgagatcactctgcgtggtcaggtactgccgatcggtggtttgaaagaaaaactcctggcagcgcatcgcggcgggatt
aaaacagtgctaattccgttcgaaaataaacgcgatctggaagagattcctgacaacgtaattgccgatctggacattc
atcctgtgaagcgcattgaggaagttctgactctggcgctgcaaaatgaaccgtctggtatgcaggttgtgactgcaaa
atagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata