LOCUS       BBa_K185004              443 bp    DNA     linear       18-Nov-2024
DEFINITION  RelE toxin+Double terminator
ACCESSION   BBa_K185004
VERSION     BBa_K185004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start
     CDS             4..285
                     /label=cds
     misc_feature    306..306
                     /label=stop
     misc_feature    305..306
                     /label=mutation
     misc_feature    286..303
                     /label=His tag
     CDS             1..306
                     /label=BBa_K185047
     stem_loop       326..369
                     /label=stem_loop
     misc_feature    315..394
                     /label=BBa_B0010
     terminator      315..394
                     /label=BBa_B0010
     stem_loop       410..429
                     /label=T7 TE
     misc_feature    436..436
                     /label=stop
     polyA_site      430..443
                     /label=polya
     misc_feature    403..443
                     /label=BBa_B0012
     terminator      403..443
                     /label=BBa_B0012
ORIGIN
        1 atggcgtatt ttctggattt tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg
       61 acggtacgtg aacagttgaa aaagaagctg gttgaagtac ttgagtcacc ccggattgaa
      121 gcaaacaagc tccgtggtat gcctgattgt tacaagatta agctccggtc ttcaggctat
      181 cgccttgtat accaggttat agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa
      241 agagaacgct cggaagtata tagcgaggcg gtcaaacgca ttctccatca tcatcatcat
      301 cactaatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      361 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      421 gggtgggcct ttctgcgttt ata
//