LOCUS BBa_K185005 3250 bp DNA linear 18-Nov-2024 DEFINITION Tryptophan-Induced RelB-antitoxin&Arabinose-Induced RelE-toxin ACCESSION BBa_K185005 VERSION BBa_K185005.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23116 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..772 /label=cI lambda misc_feature 773..805 /label=LVA misc_feature 812..836 /label=Help:Barcodes CDS 62..811 /label=BBa_C0051 stem_loop 856..899 /label=stem_loop misc_feature 845..924 /label=BBa_B0010 terminator 845..924 /label=BBa_B0010 stem_loop 940..959 /label=T7 TE misc_feature 966..966 /label=stop polyA_site 960..973 /label=polya misc_feature 933..973 /label=BBa_B0012 terminator 933..973 /label=BBa_B0012 promoter 1019..1024 /label=-10 promoter 996..1001 /label=-35 misc_feature 1006..1022 /label=OR1 misc_feature 982..998 /label=OR2 misc_feature 982..1030 /label=BBa_R0051 promoter 982..1030 /label=BBa_R0051 misc_feature 44..1030 /label=BBa_Q04510 misc_feature 1043..1046 /label=conserved RBS 1039..1050 /label=BBa_B0034 misc_feature 1057..1059 /label=start CDS 1057..1296 /label=cds misc_feature 1312..1314 /label=stop misc_feature 1297..1311 /label=His tag CDS 1057..1314 /label=BBa_K185048 stem_loop 1334..1377 /label=stem_loop misc_feature 1323..1402 /label=BBa_B0010 terminator 1323..1402 /label=BBa_B0010 stem_loop 1418..1437 /label=T7 TE misc_feature 1444..1444 /label=stop polyA_site 1438..1451 /label=polya misc_feature 1411..1451 /label=BBa_B0012 terminator 1411..1451 /label=BBa_B0012 misc_feature 1..1451 /label=BBa_K185034 promoter 1460..2801 /label=BBa_K185052 misc_feature 2808..2810 /label=start CDS 2811..3092 /label=cds misc_feature 3113..3113 /label=stop misc_feature 3112..3113 /label=mutation misc_feature 3093..3110 /label=His tag CDS 2808..3113 /label=BBa_K185047 stem_loop 3133..3176 /label=stem_loop misc_feature 3122..3201 /label=BBa_B0010 terminator 3122..3201 /label=BBa_B0010 stem_loop 3217..3236 /label=T7 TE misc_feature 3243..3243 /label=stop polyA_site 3237..3250 /label=polya misc_feature 3210..3250 /label=BBa_B0012 terminator 3210..3250 /label=BBa_B0012 misc_feature 1460..3250 /label=BBa_K185001 ORIGIN 1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta 61 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa 121 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa 181 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc 241 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc 301 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag 361 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag 421 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc 481 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag 541 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt 601 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg 661 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg 721 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gcgctgcaaa 781 cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta gatcgctact 841 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 901 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 961 ttctgcgttt atatactaga gtaacaccgt gcgtgttgac tattttacct ctggcggtga 1021 taatggttgc tactagagaa agaggagaaa tactagatgg gtagcattaa cctgcgtatt 1081 gacgatgaac ttaaagcgcg ttcttacgcc gcgcttgaaa aaatgggtgt aactccttct 1141 gaagcgcttc gtctcatgct cgagtatatc gctgacaatg aacgcttgcc gttcaaacag 1201 acactcctga gtgatgaaga tgctgaactt gtggagatag tgaaagaacg gcttcgtaat 1261 cctaagccag tacgtgtgac gctggatgaa ctccatcatc atcatcatca ctgatactag 1321 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 1381 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 1441 ctgcgtttat atactagagc gatgcataat gtgcctgtca aatggacgaa gcagggattc 1501 tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca 1561 acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg 1621 ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc 1681 gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac 1741 gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca 1801 gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg 1861 ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga 1921 gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca 1981 gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc 2041 tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg 2101 gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc 2161 attcgcgagc ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa 2221 tatcacccgg tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa 2281 tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat 2341 aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg 2401 gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag 2461 aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct 2521 cgctaaccaa accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag 2581 ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt 2641 atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc 2701 ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt ttttgggcta 2761 gaaataattt tgtttaactt taagaaggag atatacatat gtactagatg gcgtattttc 2821 tggattttga cgagcgggca ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac 2881 agttgaaaaa gaagctggtt gaagtacttg agtcaccccg gattgaagca aacaagctcc 2941 gtggtatgcc tgattgttac aagattaagc tccggtcttc aggctatcgc cttgtatacc 3001 aggttataga cgagaaagtt gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg 3061 aagtatatag cgaggcggtc aaacgcattc tccatcatca tcatcatcac taatactaga 3121 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 3181 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 3241 tgcgtttata //