##gff-version 3
##sequence-region BBa_K185005 1 3250
BBa_K185005	.	engineered_region	1	1451	.	+	0	ID=BBa_K185034;Name=BBa_K185034
BBa_K185005	.	terminator	1323	1402	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K185034
BBa_K185005	.	stem_loop	1334	1377	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K185005	.	engineered_region	1323	1402	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K185005	.	ribosome_entry_site	1039	1050	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K185034
BBa_K185005	.	conserved_region	1043	1046	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K185005	.	terminator	1411	1451	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K185034
BBa_K185005	.	polyA_site	1438	1451	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K185005	.	stem_loop	1418	1437	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K185005	.	stop_codon	1444	1444	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K185005	.	engineered_region	1411	1451	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K185005	.	promoter	1	35	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_K185034
BBa_K185005	.	engineered_region	44	1030	.	+	0	ID=BBa_Q04510;Name=BBa_Q04510;Parent=BBa_K185034
BBa_K185005	.	CDS	62	811	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_Q04510
BBa_K185005	.	tag	773	805	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K185005	.	CDS	65	772	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K185005	.	engineered_tag	812	836	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K185005	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04510
BBa_K185005	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K185005	.	terminator	845	924	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04510
BBa_K185005	.	stem_loop	856	899	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K185005	.	engineered_region	845	924	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K185005	.	promoter	982	1030	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_Q04510
BBa_K185005	.	engineered_region	982	1030	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K185005	.	promoter	1019	1024	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K185005	.	promoter	996	1001	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K185005	.	non_covalent_binding_site	1006	1022	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K185005	.	non_covalent_binding_site	982	998	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K185005	.	terminator	933	973	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04510
BBa_K185005	.	polyA_site	960	973	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K185005	.	stem_loop	940	959	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K185005	.	stop_codon	966	966	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K185005	.	engineered_region	933	973	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K185005	.	CDS	1057	1314	.	+	0	ID=BBa_K185048;Name=BBa_K185048;Parent=BBa_K185034
BBa_K185005	.	CDS	1057	1296	.	+	0	ID=annotation2046600;Name=cds;Parent=BBa_K185048
BBa_K185005	.	start_codon	1057	1059	.	+	0	ID=annotation2046599;Name=start;Parent=BBa_K185048
BBa_K185005	.	stop_codon	1312	1314	.	+	0	ID=annotation2046800;Name=stop;Parent=BBa_K185048
BBa_K185005	.	tag	1297	1311	.	+	0	ID=annotation2046993;Name=His tag;Parent=BBa_K185048
BBa_K185005	.	engineered_region	1460	3250	.	+	0	ID=BBa_K185001;Name=BBa_K185001
BBa_K185005	.	terminator	3210	3250	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K185001
BBa_K185005	.	polyA_site	3237	3250	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K185005	.	stem_loop	3217	3236	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K185005	.	stop_codon	3243	3243	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K185005	.	engineered_region	3210	3250	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K185005	.	CDS	2808	3113	.	+	0	ID=BBa_K185047;Name=BBa_K185047;Parent=BBa_K185001
BBa_K185005	.	tag	3093	3110	.	+	0	ID=annotation2047133;Name=His tag;Parent=BBa_K185047
BBa_K185005	.	sequence_alteration	3112	3113	.	+	0	ID=annotation2047034;Name=mutation;Parent=BBa_K185047
BBa_K185005	.	start_codon	2808	2810	.	+	0	ID=annotation2046533;Name=start;Parent=BBa_K185047
BBa_K185005	.	CDS	2811	3092	.	+	0	ID=annotation2046560;Name=cds;Parent=BBa_K185047
BBa_K185005	.	stop_codon	3113	3113	.	+	0	ID=annotation2046574;Name=stop;Parent=BBa_K185047
BBa_K185005	.	promoter	1460	2801	.	+	0	ID=BBa_K185052;Name=BBa_K185052;Parent=BBa_K185001
BBa_K185005	.	terminator	3122	3201	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K185001
BBa_K185005	.	stem_loop	3133	3176	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K185005	.	engineered_region	3122	3201	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K185005
ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgagcacaaaaaagaaa
ccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttat
cccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaa
tgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatc
tacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcagg
cagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccag
tgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacgga
atgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagttta
ccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaa
tgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaac
tacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggt
tgctactagagaaagaggagaaatactagatgggtagcattaacctgcgtattgacgatgaacttaaagcgcgttctta
cgccgcgcttgaaaaaatgggtgtaactccttctgaagcgcttcgtctcatgctcgagtatatcgctgacaatgaacgc
ttgccgttcaaacagacactcctgagtgatgaagatgctgaacttgtggagatagtgaaagaacggcttcgtaatccta
agccagtacgtgtgacgctggatgaactccatcatcatcatcatcactgatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagcgatgcataatgtgcctgtcaaatggacgaagcagggattct
gcaaaccctatgctactccgtcaagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattc
actttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagtt
gatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgat
acgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaag
caaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgta
cccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttat
cgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctgg
tgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcg
gacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaa
cagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattg
agaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccg
ccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgcc
attcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaacc
aaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtg
tctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccat
aagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagaaat
aattttgtttaactttaagaaggagatatacatatgtactagatggcgtattttctggattttgacgagcgggcactaa
aggaatggcgaaagctgggctcgacggtacgtgaacagttgaaaaagaagctggttgaagtacttgagtcaccccggat
tgaagcaaacaagctccgtggtatgcctgattgttacaagattaagctccggtcttcaggctatcgccttgtataccag
gttatagacgagaaagttgtcgttttcgtgatttctgttgggaaaagagaacgctcggaagtatatagcgaggcggtca
aacgcattctccatcatcatcatcatcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttata