LOCUS       BBa_K185007             1514 bp    DNA     linear       19-Dec-2024
DEFINITION  Promoter116+RBS34+LacI+Double-terminator+Plac+RBS31
ACCESSION   BBa_K185007
VERSION     BBa_K185007.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23116
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1147
                     /label=BBa_I732100
     stem_loop       1167..1210
                     /label=stem_loop
     misc_feature    1156..1235
                     /label=BBa_B0010
     terminator      1156..1235
                     /label=BBa_B0010
     stem_loop       1251..1270
                     /label=T7 TE
     misc_feature    1277..1277
                     /label=stop
     polyA_site      1271..1284
                     /label=polya
     misc_feature    1244..1284
                     /label=BBa_B0012
     terminator      1244..1284
                     /label=BBa_B0012
     CDS             1293..1380
                     /label=end of LacI coding region (inactive)
     misc_feature    1293..1492
                     /label=BBa_R0010
     misc_feature    1381..1418
                     /label=CAP binding site
     promoter        1429..1434
                     /label=-35
     promoter        1453..1458
                     /label=-10
     misc_feature    1458..1492
                     /label=LacI binding site
     misc_feature    1465..1465
                     /label=start
     promoter        1293..1492
                     /label=BBa_R0010
     misc_feature    1507..1510
                     /label=conserved
     RBS             1501..1514
                     /label=BBa_B0031
ORIGIN
        1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta
       61 gatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
      121 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
      181 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
      241 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
      301 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
      361 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
      421 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
      481 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
      541 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
      601 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
      661 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
      721 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
      781 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
      841 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc
      901 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
      961 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
     1021 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     1081 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     1141 gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1201 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1261 cgggtgggcc tttctgcgtt tatatactag agcaatacgc aaaccgcctc tccccgcgcg
     1321 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     1381 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     1441 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag
     1501 tcacacagga aacc
//